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Development of novel therapy against food allergy in animals.

开发针对动物食物过敏的新疗法。

基本信息

批准号：
13556051
负责人：
MASUDA Kenichi
金额：
$ 9.02万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

In this study, pathogenesis of food allergy in dogs and cats has been investigated with immunological techniques. We found that types I and IV hypersensitivity against food allergens were involved in the pathogenesis of food hypersensitivity in dogs and cats. In terms of cross-reactivity between food and environmental allergens, oral allergy syndrome was reported in a dog sensitive to Japanese cedar pollen as a consequence of cross-reactivity of IgE between the cedar pollen and tomato antigen. We also developed an objective marker to identify offending food allergens by lymphocyte blastogenic responses against food allergens, resulting in a major contribution to veterinary clinical medicine for the diagnosis of food hypersensitivity in dogs. On the other hand, anaphylaxis against canine vaccine was epidemiologically suggested to be associated with food hypersensitivity in dogs. In order to develop novel immunotherapies, dogs experimentally induced allergy showed reduction of bronchial hypersensitivity to allergens by oral immunotherapy using T cell epitopes. We also found that a sequence of CpG motife that effectively induced IFN-g production from peripheral blood mononuclear lymphocytes.Thus, in this study, a major part of pathogenesis of food hypersensitivity in dogs and cats was clearly elucidated, contributing to veterinary clinical medicine. In addition, since the pathogenesis of food hypersensitivity in dogs and cats was very similar to that in humans, these animals would be useful as a nanimal model of spontaneous food allergy.

本研究应用免疫学技术对犬、猫食物过敏的发病机制进行了研究。我们发现，对食物过敏原的I型和IV型超敏反应参与了犬和猫食物过敏的发病机制。在食物和环境过敏原之间的交叉反应性，口腔过敏综合征的狗敏感的日本雪松花粉的雪松花粉和番茄抗原之间的IgE交叉反应性的结果。我们还开发了一种客观的标记物，以确定得罪的食物过敏原的淋巴细胞母细胞反应对食物过敏原，从而在兽医临床医学的诊断狗的食物过敏症的重大贡献。另一方面，对犬疫苗的过敏反应在流行病学上被认为与犬的食物过敏有关。为了开发新的免疫疗法，通过使用T细胞表位的口服免疫疗法，实验诱导的过敏症的狗显示出对过敏原的支气管超敏性的降低。我们还发现了一个能有效诱导外周血单个核淋巴细胞产生IFN-γ的CpG基序序列，从而阐明了犬、猫食物过敏的主要发病机制，为兽医临床用药提供了理论依据。此外，由于狗和猫的食物过敏的发病机制与人类非常相似，这些动物将是有用的自发性食物过敏的nanimal模型。