Clinical Study and Developement of Allograft Mitral Valve with Durability and Non-thrombosis for canine valve Replacement(2003)

犬瓣膜置换术中耐用、无血栓同种异体二尖瓣的临床研究与开发（2003）

• 批准号: 13556053
• 负责人: YAMANE Yoshihisa
• 金额: $ 8.96万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13556053/
• 关键词: Dog; Mitral Insufficiency; allograft valve; valve Replacement; cardiopulmonary bypass system; 体外循環; 人工心肺装置

Recently, requirement of the the open heart surgery in small animals are on the increase. Especilly, the requirement increase on the congenital,and acquired heart diseases.In humans, current technique in open heart surgery involve establishing extracorporeal circulation using an artificial heart-lung machine. But, the techniques of cardiopulmonary bypass system in veterinary domain had not established.We established the technique of extracorporeal circulation in dogs on several years ago.The other side, the incidence of canine mitral insufficiency (regurgitation) of acquired heart diseases is increasing with ageing. Therefore, requirement of the radical surgery is advanced by client.The purpose of this study is establishment the technique of mitral valve replacement for canine mitral insufficiency.Until now, we made allograft valve by pig's aortic valve which were treated by Denacl^R EX-313, Heparin and Protamine.The allograft valve replacement was done under the extracorporeal circulation using a cardiopulmonary bypass system.The surgical procedure was established, but the valve durability was weakly. Therefore, the allograft valve were treated by several kinds of methods and solutions. As the result, One type of these valves which were treated by Denacol and glutalaldehydes had good hemodynamic properties, high durability and in addition had low rates of thromboembolism without anticoagulation.After operation, these dogs which were done mitral valve replacement survived for about 3 years.In the future, as the result of this experimental study, mitral valve replacement for mitral insufficiency in dogs will be done in veterinary clinical field.

近年来，小动物心脏直视手术的需求量不断增加。特别是对先天性和后天性心脏病的需求增加，在人类中，目前的心脏直视手术技术涉及使用人工心肺机建立体外循环。但是，兽医领域的体外循环技术还没有建立起来，我们在几年前建立了犬体外循环技术，另一方面，随着年龄的增长，犬获得性心脏病二尖瓣关闭不全（反流）的发生率也在增加。本研究的目的是建立一种治疗犬二尖瓣关闭不全的二尖瓣置换术技术，采用猪主动脉瓣制作同种异体瓣膜，经Denacl、REX-313处理，同种异体瓣膜置换术在体外循环下进行，采用体外循环系统。建立，但瓣膜耐久性较弱。因此，同种异体瓣膜的处理方法有多种。结果表明，用Denacol和戊二醛处理的一种瓣膜具有良好的血流动力学特性，耐久性高，而且在不抗凝的情况下血栓栓塞率低，这些二尖瓣置换术后的狗存活了约3年。将在兽医临床领域进行犬二尖瓣关闭不全的二尖瓣置换术。

项目成果

Y.Noishiki, Y.Ichikawa, T.Kosuge, I.Yamazaki, K.Yamamoto, T.Manabe, Y.Lu, Y.Yamane: "Introduction of Tissue Engineering Concepts into the Field of Endovascular Grafts. : An attempt to solve endoleakage problems of endovas-cular grafts implanted in aortic

Y.Noishiki、Y.Ichikawa、T.Kosuge、I.Yamazaki、K.Yamamoto、T.Manabe、Y.Lu、Y.Yamane：“将组织工程概念引入血管内移植领域。：尝试解决

Hoshi Katsuichiro: "Comparison of extracapillary and endocapillary blood flow oxygenators for open heart surgery in dogs"J.Vet.Med.Sci.. 65(3). 357-361 (2003)

Hoshi Katsuichiro：“用于犬心脏直视手术的毛细血管外和毛细血管内血流氧合器的比较”J.Vet.Med.Sci. 65(3)。

H.Matsumoto, S.Sugiyama, A.Shibazaki, R.Tanaka, K.Takashima, Y.Noishiki, Y.Yamane: "Experimental Study of Materials for Patch Graft on Right Ventricular Outflow Tract under Extracorporeal Circulation in Dogs-Comparison between Dena-col^R EX-313-Treated Bo

H.Matsumoto、S.Sugiyama、A.Shibazaki、R.Tanaka、K.Takashima、Y.Noishiki、Y.Yamane：“狗体外循环下右心室流出道补片移植材料的实验研究 - Dena 之间的比较

Masayuki KOBAYASHI, Kiyomi WATANABE, Hidehiro HIRAO, Miki SHIMIZU, Shunsuke SHIMAMURA, Ryou TANAKA, Yoshihisa YAMANE: "Effects of β-Blocker Metoprolol on Left ventricular Function and Geometry in Dogs with Experimental Mitral Regurgitation"Advances in Aim

Masayuki KOBAYASHI、Kiyomi WATANABE、Hidehiro HIRAO、Miki SHIMIZU、Shunsuke SHIMAMURA、Ryou TANAKA、Yoshihisa YAMANE：“β-受体阻滞剂美托洛尔对实验性二尖瓣反流犬左心室功能和几何形状的影响”目标进展

Y.Nagashima, H.Hirao, S.Furukawa, K.Hoshi, M.Akahane, R.Tanaka, Y.Yamane: "Plasma Digoxin Concentration in Dogs with Mitral Regurgitation"J.Vet.Med.Sci.. 63(11). 1199-1202 (2001)

Y.Nagashima、H.Hirao、S.Furukawa、K.Hoshi、M.Akahane、R.Tanaka、Y.Yamane：“患有二尖瓣反流的狗的血浆地高辛浓度”J.Vet.Med.Sci.. 63（11）