Immortalization of human somatic cells ; possible stem cells.

人类体细胞的永生化；

• 批准号: 13557206
• 负责人: IDE Toshinori
• 金额: $ 7.74万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557206/
• 关键词: human somatic cell; immortalization; telomere; telomerase; proliferative life span; endothelial cell; astrocyte; hepatocyte

Such human somatic cells as fibroblasts, umbirical vein endothelial cells, astrocytes, and hepatocytes were immortalized by an introduction with human telomerase reverse transcriptase (hTERT) gene. Only an exception was osteoblast which could not be extended proliferative lifespan at all by hTERT introduction. All hTERT-introduced immortal cells showed normal phenotypes. Immortalized hepatocytes expressed bile duct specific markers in addition to hepatocyte specific markers, suggesting characteristics of stem cells which are able to differentiate into both hepatocytes and bile duct cells.

通过导入人端粒酶逆转录酶（hTERT）基因，使成纤维细胞、脐静脉内皮细胞、星形胶质细胞、肝细胞等人体细胞永生化。唯一的例外是成骨细胞，它根本不能通过引入 hTERT 来延长增殖寿命。所有引入 hTERT 的永生细胞均显示正常表型。除了肝细胞特异性标记之外，永生化肝细胞还表达胆管特异性标记，这表明干细胞具有能够分化为肝细胞和胆管细胞的特征。

项目成果

Okubo, M., et al.: "Clonal chromosomal aberrations accompanied by strong telomerase activity in immortalization of human B-lymphoblastoid cell lines transformed by Epstein-Barr virus"Cancer Genet. Cytogenet.. 129. 30-34 (2001)

Okubo, M., 等人：“由 Epstein-Barr 病毒转化的人 B 淋巴母细胞系永生化过程中伴随强端粒酶活性的克隆染色体畸变”Cancer Genet。

Okubo M., et al.: "Clonal chromosomal aberrations accompanied by strong telomerase activity in immortalization of human B-lymphoblastoid cell lines transformed by Epstein-Barr virus"Canqer Genet. Cytogenet.. 129. 30-34 (2001)

Okubo M.等人：“由 Epstein-Barr 病毒转化的人 B 淋巴母细胞系永生化过程中伴随强端粒酶活性的克隆染色体畸变”Canqer Genet。

Okubo M, et al.: "Clonal chromosomal aberrations accompanied by strong lelomerase activity in immortalization of human B-lymphoblastoid cell lines transformed by Epstein-Barr virus"Cancer Genet Cytogenet.. 129. 30-34 (2001)

Okubo M 等人：“在由 Epstein-Barr 病毒转化的人 B 淋巴母细胞系的永生化过程中，伴随着强利洛姆酶活性的克隆染色体畸变”Cancer Genet Cytogenet.. 129. 30-34 (2001)

Tsuyama N, et al.: "X-rays induce dose-dependent and cell cycle ? independent accumulation of p21(sdi1/ WAF1)"Hiroshima J Med Sci.. 50. 1-7 (2001)

Tsuyama N 等人：“X 射线诱导剂量依赖性和细胞周期？独立的 p21(sdi1/WAF1) 积累”Hiroshima J Med Sci.. 50. 1-7 (2001)

Y.Kanzaki: "Telomerase rescues the expression levels of keratinocyte growth factor and insulin-like growth factor-II in senescent human fibroblasts"Exp. Cell Res.. 279. 321-329 (2002)

Y.Kanzaki：“端粒酶可恢复衰老人成纤维细胞中角质形成细胞生长因子和胰岛素样生长因子-II 的表达水平”Exp。