The research on the evaluation method for the endocrine-disrupting effect of benzo[a]pyrene and its metabolites

苯并[a]芘及其代谢物内分泌干扰作用评价方法的研究

基本信息

  • 批准号:
    13558066
  • 负责人:
  • 金额:
    $ 6.27万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2001
  • 资助国家:
    日本
  • 起止时间:
    2001 至 2002
  • 项目状态:
    已结题

项目摘要

Polycyclic aromatic hydrocarbons (PAHs) such as benzo[a]pyrene (BaP) have been reported for their endocrine-disrupting activities, primarily their antiestrogenic characteristics. In this study, the endocrine-disrupting activities of PAHs and their analogues (metabolites) were investigated. Several PAHs containing BaP exhibited the antiestrogenic and antiandrogenic effects and it was suggested that the PAHs act as antiestrogens and antiandrogens by activating the aryl hydrocarbon receptor (AhR). On the other hand, the estrogenic and antiestrogenic effects of the BaP metabolites were also examined. Monohydroxy derivatives of BaP (OHBaPs) exhibited the estrogenic or antiestrogenic effects, diol and quinone derivatives of BaP did not. Therefore, the presence and position of phenolic hydroxy group in the BaP metabolites play an important role in the induction of the endocrine-disrupting activities.Two methods for the evaluation of PAH exposure were developed to assess a human risk of the en … More docrine-disrupting effects for PAHs and their metabolites. To evaluate the exposure to PAHs from ambient air by inhalation, the atmospheric concentrations of PAHs were measured with the airborne particulates collected in three cities. The concentrations of PAHs, that is, the exposure dose were much higher in urban area than in suburban area, and the main contributors of PAHs in urban area were considered to be automobiles, domestic heating systems such as furnaces and kerosene heaters, power plants and incinerators. However, the intake of PAHs depends on individual lifestyle such as diet and tobacco smoke, therefore, biomonitoring is an especially valuable method to provide exposure data of PAHs. Urinary metabolites of PAHs including BaP were used as PAH biomarkers. 1-, 3-, 9-OHBaPs were identified as major metabolites of BaP in vitro by human recombinant cytochrome P450. The methods for determining OHBaPs, 1-hydroxypyrene (1-OHP) and 2-hydroxyfluorene (2-OHF) using high-performance liquid chromatography with fluorescence detection were developed. 3-OHBaP, which has a particularly high estrogenic activity, was detected in the urine of a subject. Further, the effect of smoking on the exposure to PAHs was investigated using the concentrations of urinary 1-OHP and 2-OHF. The urinary 1-OHP and 2-OHF levels in smokers were significantly higher than those in non-smokers. Thus, urinary metabolites of PAHs may be sensitive and specific biomarkers for the assessment of the exposure to PAHs. Less
多环芳烃(PAHs)如苯并[a]芘(BaP)因其内分泌干扰活性,主要是其抗雌激素特性而被报道。本研究对多环芳烃及其类似物(代谢物)的内分泌干扰活性进行了研究。几种含BaP的多环芳烃具有抗雌激素和抗雄激素的作用,表明多环芳烃可能通过激活芳烃受体(AhR)而发挥抗雌激素和抗雄激素的作用。另一方面,还研究了BaP代谢物的雌激素和抗雌激素作用。BaP的单羟基衍生物(OHBaPs)具有雌激素或抗雌激素作用,而BaP的二醇类和醌类衍生物则没有。因此,酚羟基在BaP代谢产物中的存在和位置在诱导内分泌干扰活动中起着重要作用。多环芳烃暴露的两种评估方法被开发出来评估人类对多环芳烃及其代谢物的影响。为评价多环芳烃的吸入性,对3个城市采集的大气颗粒物进行了多环芳烃浓度测定。城市多环芳烃浓度(即暴露剂量)明显高于郊区,城市多环芳烃的主要来源为汽车、家用供暖系统(如炉炉、煤油加热器)、发电厂和焚化炉。然而,多环芳烃的摄入量取决于个人的生活方式,如饮食和吸烟,因此,生物监测是提供多环芳烃暴露数据的一种特别有价值的方法。多环芳烃尿液代谢产物包括BaP作为多环芳烃生物标志物。1-, 3-, 9-OHBaPs是体外重组细胞色素P450鉴定的BaP的主要代谢物。建立了荧光检测高效液相色谱法测定ohaps、1-羟基芘(1-OHP)和2-羟基芴(2-OHF)的方法。在一名受试者的尿液中检测到具有特别高的雌激素活性的3-OHBaP。此外,吸烟对多环芳烃暴露的影响通过尿中1-OHP和2-OHF的浓度进行了研究。吸烟者尿中1-OHP和2-OHF水平明显高于非吸烟者。因此,尿中多环芳烃的代谢物可能是评估多环芳烃暴露的敏感和特异性生物标志物。少

项目成果

期刊论文数量(84)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ning Tang: "Comparison of Atmospheric Polycyclic Aromatic Hydrocarbons in Vladivostok, Toyama and Kanazawa"Bulletin of the Japan Sea Research Institute of Kanazawa University. 33. 77-86 (2002)
唐宁:《海参崴、富山、金泽大气多环芳烃对比》金泽大学日本海洋研究所通报。
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    0
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Kazumasa Okamura: "Antiestrogenic Activity of Extracts of Diesel Exhaust Particulate Matter in MCF-7 Human Breast Carcinoma Cells."Polycyclic Aromatic Compounds. 22. 747-759 (2002)
Kazumasa Okamura:“MCF-7 人乳腺癌细胞中柴油机尾气颗粒物提取物的抗雌激素活性。”多环芳香族化合物。
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    0
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Hirose, T., Kizu, R., Toriba, A., Hayakawa, K., Masamune, Y, et al.: "Estrogenic/Antiestrogenic Activities of Benzo[a]pyrene Monohydroxy Derivatives"J. Health Sci.. 47(6). 552-558 (2001)
Hirose, T.、Kizu, R.、Toriba, A.、Hayakawa, K.、Masamune, Y 等人:“苯并[a]芘单羟基衍生物的雌激素/抗雌激素活性”J。
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    0
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Hitoshi Kakimoto: "Considerations of Atmospheric Behaviors of Polycyclic Aromatic Hydrocarbons, Nitropolycyclic Aromatic Hydrocarbons and Inorganic Pollutants Based on Their Interrelationships"Journal of Health Science. 47. 385-393 (2001)
Hitoshi Kakimoto:“基于相互关系的多环芳烃、硝基多环芳烃和无机污染物的大气行为的考虑”健康科学杂志。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Ning Tang: "Comparison of Atmospheric Polycyclic Aromatic Hydrocarbons in Vladivostok, Toyama and Kanazawa."Bulletin of the Japan Sea Research, Institute of Kanazwa University. 33. 77-86 (2002)
唐宁:“符拉迪沃斯托克、富山和金泽大气多环芳烃的比较。”金泽大学研究所日本海洋研究所通报。
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    0
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TORIBA Akira其他文献

TORIBA Akira的其他文献

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{{ truncateString('TORIBA Akira', 18)}}的其他基金

Development of a method for assessing human exposure to cross-boundary air pollutions by polycyclic aromatic sulfur heterocyclic compounds as chemical markers
以多环芳香族硫杂环化合物为化学标记物,开发评估人体跨境空气污染暴露的方法
  • 批准号:
    24590160
  • 财政年份:
    2012
  • 资助金额:
    $ 6.27万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
International comparative research on exposure and biological effects of air pollution for local residents in Asia
亚洲当地居民空气污染暴露及其生物效应的国际比较研究
  • 批准号:
    23406004
  • 财政年份:
    2011
  • 资助金额:
    $ 6.27万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of a biomarker for assessing exposure to quinone compounds associated with producing reactive oxygen species
开发用于评估与产生活性氧相关的醌化合物暴露的生物标记物
  • 批准号:
    21790126
  • 财政年份:
    2009
  • 资助金额:
    $ 6.27万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
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