Practical use of cloning technology for NOD and dy disease model mice production

本研究旨在利用胚胎干细胞建立胚胎克隆系统，为疾病及其治疗的基础研究做出贡献。本研究以NOD非肥胖糖尿病小鼠作为糖尿病ⅰ型模型，dy和mdx小鼠作为肌肉萎缩症模型，并尝试用上述疾病模型小鼠衍生的ES细胞制备克隆小鼠。所得结果总结如下：1)通过标准核移植，利用NOD小鼠胚胎干细胞成功制备了克隆囊胚，但尚未获得幼仔。2)从dy和mdx小鼠身上建立了可多次培养的胚胎干细胞。3)利用这些胚胎干细胞作为供核，获得了克隆囊胚，但仍未获得幼仔。我们一起从NOD、dy和mdx小鼠身上建立了胚胎干细胞，但未能产生克隆个体。可能需要进一步的研究来阐明这里建立的胚胎干细胞的发育能力。

The present study was conducted to develop embryo cloning systems using ES cell and contribute to fundamental studies for disease and its treatments. Here, we focused on NOD non-obese diabetic mouse used as a model for diabetes typeI, and dy and mdx mice used as muscular dystrophy and tried to produce cloned mice from ES cells derived from above disease model mice. The results obtained were summarized as follows. 1)By standard nuclear transfer, cloned blastocysts were successfully produced using ES cells established from NOD mice, but no pup was obtained to date. 2)ES cells, which can be cultured over several passages, have been established from dy and mdx mice. 3)Using these ES cells as donor nuclei, cloned blastocysts were produced, but again no pup was obtained. Together, we have established ES cells from NOD, dy and mdx mice, but failed to produce cloned individuals. Further studies may be necessary to clarify developmental competence of ES cells established here.