Practical use of cloning technology for NOD and dy disease model mice production

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The present study was conducted to develop embryo cloning systems using ES cell and contribute to fundamental studies for disease and its treatments. Here, we focused on NOD non-obese diabetic mouse used as a model for diabetes typeI, and dy and mdx mice used as muscular dystrophy and tried to produce cloned mice from ES cells derived from above disease model mice. The results obtained were summarized as follows. 1)By standard nuclear transfer, cloned blastocysts were successfully produced using ES cells established from NOD mice, but no pup was obtained to date. 2)ES cells, which can be cultured over several passages, have been established from dy and mdx mice. 3)Using these ES cells as donor nuclei, cloned blastocysts were produced, but again no pup was obtained. Together, we have established ES cells from NOD, dy and mdx mice, but failed to produce cloned individuals. Further studies may be necessary to clarify developmental competence of ES cells established here.