Application of Copolymers of Lactic Acid and Amino Acid Having Reactive Side-Chain Groups as Biodegradable Materials for Tissue Engineering

具有反应性侧链基团的乳酸和氨基酸共聚物作为可生物降解材料在组织工程中的应用

基本信息

  • 批准号:
    13558120
  • 负责人:
  • 金额:
    $ 9.02万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2001
  • 资助国家:
    日本
  • 起止时间:
    2001 至 2003
  • 项目状态:
    已结题

项目摘要

In tissue engineering related to the regeneration of damaged or lost tissue, excellent biodegradable materials are desired as temporary scaffolds to support cell growth and disappear with the progress of tissue regeneration. We previously synthesized biodegradable poly(depsipeptide-random-lactide), poly[(Glc-Asp)-r-LA] and poly[(Glc-Lys)-r-LA] having reactive side-chain groups. In this study, to evaluate the utility of these copolymers as functional scaffolds for tissue regeneration, the effects of reactive and ionic side-chain groups on cell attachment and growth were investigated using copolymer films with various amounts of carboxyl or amino groups. Poly[(Glc-Lys)-r-LA] and poly[(Glc-Asp)-r-LA] films having appropriate positive or negative charges exhibited higher cell attachment ability than poly-L-lactide. Good cell growth was observed on the copolymer films. During cell culture, the copolymer films exhibited higher degradation rates related to the depsipeptide unit content. Biodegradable polymer matrices for cell growth, with reactive surfaces and controllable degradability, were successfully prepared using copolymers with various amounts of depsipeptide units. Moreover, to evaluate the utility of these copolyrners as functional scaffolds for tissue regeneration, three-dimensional porous sponges were prepared by freeze-drying method and the effects of reactive and ionic side-chain groups on cell growth and degradation behavior were investigated using copolymer sponges with various amounts of carboxyl or amino groups. Good cell growth was observed on the copolymer sponges. During cell culture, the copolymer sponges exhibited various degradation rates related to the depsipeptide unit content. Three-dimensional biodegradable polymer matrices with reactive surface, controllable degradation behavior and good cell growth were successfully prepared using these copolymers. Such kinds of copolymer matrices are good candidate for scaffold for tissue engineering.
在与损伤或丢失的组织再生相关的组织工程中,人们希望使用性能优良的生物可降解材料作为临时支架,以支持细胞生长,并随着组织再生的进行而消失。我们以前合成了具有反应性侧链基团的可生物降解的聚(去脂肽-随机-丙交酯)、聚[(GLC-Asp)-r-LA]和聚[(GLC-Lys)-r-LA]。在这项研究中,为了评价这些共聚物作为组织再生功能支架的有效性,使用含有不同数量的羧基或氨基的共聚物膜,研究了反应性侧链和离子侧链对细胞附着和生长的影响。具有适当正负电荷的聚[(Glc-Lys)-r-LA]和聚[(Glc-Asp)-r-LA]薄膜比聚L-丙交酯具有更强的细胞黏附能力。共聚物膜上有良好的细胞生长。在细胞培养过程中,共聚物膜表现出较高的降解率,其降解率与去脂肽单元含量有关。利用含有不同量去脂肽单元的共聚物,成功地制备了具有活性表面和可控降解性的可生物降解的细胞生长聚合物基质。此外,为了评价这些共聚体作为组织再生功能支架的有效性,采用冷冻干燥法制备了三维多孔海绵,并用不同含量的羧基或氨基的共聚海绵研究了反应性侧链和离子侧链对细胞生长和降解行为的影响。细胞在共聚海绵上生长良好。在细胞培养过程中,共聚物海绵的降解率与脱脂多肽单位含量有关。利用这些共聚物成功地制备了表面活性好、降解行为可控、细胞生长良好的三维可生物降解聚合物基质。这类共聚物基质是组织工程支架的理想候选材料。

项目成果

期刊论文数量(61)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
大内辰郎, 大矢裕一: "新規なポリ乳酸系医用材料"未来材料. 2・3. 30-35 (2002)
大内达朗、大谷佑一:“新型聚乳酸类医疗材料”Future Materials 2・3(2002)。
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    0
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  • 通讯作者:
F.Tasaka, Y.Ohya, T.Ouchi: "One-Pot Synthesis of Novel Branched Polylactide through Copolymerization of Lactide with Mevalonolactone"Macromol. Chem. Rapid Commun.. 22・11. 820-824 (2001)
F. Tasaka、Y. Ohya、T. Ouchi:“通过丙交酯与甲瓦龙酸内酯的共聚一锅法合成新型支链聚丙交酯” Macromol Chem. 22・11 (2001)
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    0
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T.Ouchi, T.Uchida, Y.Ohya: "Synthesis of Poly(L-Lactic Acid) Having One Terminal D-Glucose Residue and Wettability of Its Film Surface"Macromol. Biosci.. 1. 371-375 (2001)
T.Ouchi、T.Uchida、Y.Ohya:“具有末端D-葡萄糖残基的聚(L-乳酸)的合成及其膜表面的润湿性”Macromol。
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  • 影响因子:
    0
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大内辰郎, 大矢裕一: "生分解性バイオマテリアルの設計および医療用材料への応用"関西大学工業技術研究所報 技苑. 108. 34-35 (2001)
Tatsuo Ouchi、Yuichi Oya:“可生物降解的生物材料的设计及其在医疗材料中的应用”关西大学工业技术研究所杂志 Gien 108. 34-35 (2001)。
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    0
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  • 通讯作者:
Y.Ohya, H.Matsunami, T.Ouchi: "Cell Attachment and Growth on Films Prepared from Poly(depsipeptide-co-lactide) Having Various Functional Groups"J.Biomed.Mater.Res.. 65A(1). 79-88 (2003)
Y.Ohya、H.Matsunami、T.Ouchi:“由具有各种官能团的聚(缩肽-丙交酯)制备的膜上的细胞附着和生长”J.Biomed.Mater.Res.. 65A(1)。
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OUCHI Tatsuro其他文献

OUCHI Tatsuro的其他文献

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{{ truncateString('OUCHI Tatsuro', 18)}}的其他基金

Application of Multi-Arm Poly (Ethylene Glycol) -Polylactide Copolymers as Biodegradable Soft Biomaterials
多臂聚乙二醇-聚丙交酯共聚物作为可生物降解软质生物材料的应用
  • 批准号:
    17300163
  • 财政年份:
    2005
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Synthesis of Polylactide with Branched Structures and Control of Its Mechanical Property& Biodegradability
支化结构聚丙交酯的合成及其力学性能的控制
  • 批准号:
    14350490
  • 财政年份:
    2002
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
SYNTHESIS of BIODEGRADABLE COLYMER OF LACTIC ACID HAVING THIOL GROUPS AND EVALUATION OF ITS CHARACTERIZATION
含硫醇基可生物降解乳酸聚合物的合成及其表征评价
  • 批准号:
    10650874
  • 财政年份:
    1998
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development on Selective Delivery System of Antitumor Agents by Utilizing Cell-Recognition Ability of Saccharides
利用糖类细胞识别能力开发抗肿瘤药物选择性递送系统
  • 批准号:
    05680767
  • 财政年份:
    1993
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Design of Macromolecular Prodrug Having Antitumor Activity
具有抗肿瘤活性的大分子前药的设计
  • 批准号:
    02650672
  • 财政年份:
    1990
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Synthesis of Malic Acid Polymer and Its Hydrolysis Behavior
苹果酸聚合物的合成及其水解行为
  • 批准号:
    61550685
  • 财政年份:
    1986
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
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