Highly-functionalization of oxine-copper analysis on the base of coordination ability of residual silanol and environmental analysis of oxine-copper

在本研究中，我们研究了一种分析黄铜的方法的发展及其在环境中的迁移分析。为此，我们利用高效液相色谱法，将硅醇基团集中在硅胶填料表面作为分离柱，通过对硅醇基团作用的研究，发现硅醇基团与三(2-甲基-8-喹啉)铁(III)或三(2-甲基-8-喹啉)镓(III)之间的相互作用。利用这些结果，我们建立了硅胶表面硅醇基团的识别方法。此外，我们还阐明了硅醇基与络合物之间的相互作用是由于硅醇基的配位作用引起的配体交换反应。这是首次对硅醇基的配位作用进行研究，我们还发现硅醇基的配位作用也会影响奥克辛-铜的洗脱。因此，选择合适的高效液相色谱柱是高效液相色谱分离奥克辛-铜的关键。我们成功地用硅醇检测探针(三(2-甲基-8-喹啉)铁(III)和三(2-甲基-8-喹啉)镓(III))找到了最合适的高效液相色谱柱。建立了一种灵敏的高效液相色谱测定环境水样中痕量铜的方法。将高效液相色谱法和JIS法相结合，可以获得一些关于恶唑铜在环境中迁移的有用信息。

In this research, we investigated the development of an analytical method of oxine-copper and the analysis of the movement of oxine-copper in environment of with the developed method. We used a high-performance liquid chromatography for the purpose and we focused silanol groups on the surface of silica gel packings used as a separation column in HPLC.We found the interaction between the silanol groups and a tris(2-methyl-8-quinolinolato)iron(III) or a tris(2-methyl-8-quinolinolato)gallium(III) through the investigation into a function of the silanol groups. We developed a discrimination method of silanol groups on the surface of silica gel with these results. Also we clarified that the interaction between the silanol groups and the complexes was due to a ligand exchange reaction arising out of a coordination function of the silanol groups. This is the first research concerning the coordination function of silanol groups.We also found that the coordination function of the silanol groups also affected the elution of oxine-copper. Therefore, a selection of a suitable HPLC column is crucial in separating oxine-copper with HPLC. We successfully found the most suitable HPLC column with the silanol-detecting probes (tris(2-methyl-8-quinolinolato)iron(III) and tris(2-methyl-8-quinolinolato)gallium(III)). We could develop a sensitive determination method of trace amount of oxine-copper in environmental waters with HPLC. Combing the HPLC method and the JIS method, it was possible to obtain some useful informations about the movement of oxine-copper in the environment.