Highly-functionalization of oxine-copper analysis on the base of coordination ability of residual silanol and environmental analysis of oxine-copper

本研究探讨了一种氧铜分析方法的建立，并利用该方法分析了氧铜在环境中的运动。我们使用高效液相色谱法，将硅醇基团集中在硅胶填料表面，作为高效液相色谱的分离柱。通过对硅烷醇基团功能的研究，我们发现了硅烷醇基团与三（2-甲基-8-喹啉酸）铁（III）或三（2-甲基-8-喹啉酸）镓（III）之间的相互作用。根据这些结果，我们建立了一种硅胶表面硅醇基的判别方法。此外，我们还澄清了硅烷醇基团与配合物之间的相互作用是由于硅烷醇基团的配位作用引起的配体交换反应。这是首次对硅烷醇基团的配位功能进行研究。我们还发现硅烷醇基团的配位功能也影响了氧铜的洗脱。因此，选择合适的高效液相色谱柱是高效液相色谱分离氧铜的关键。用三（2-甲基-8-喹啉醇）铁（III）和三（2-甲基-8-喹啉醇）镓（III）两种探针成功地找到了最合适的高效液相色谱柱。建立了高效液相色谱法测定环境水体中痕量氧铜的灵敏方法。结合高效液相色谱法和JIS法，可以获得一些有关环境中氧铜运动的有用信息。

In this research, we investigated the development of an analytical method of oxine-copper and the analysis of the movement of oxine-copper in environment of with the developed method. We used a high-performance liquid chromatography for the purpose and we focused silanol groups on the surface of silica gel packings used as a separation column in HPLC.We found the interaction between the silanol groups and a tris(2-methyl-8-quinolinolato)iron(III) or a tris(2-methyl-8-quinolinolato)gallium(III) through the investigation into a function of the silanol groups. We developed a discrimination method of silanol groups on the surface of silica gel with these results. Also we clarified that the interaction between the silanol groups and the complexes was due to a ligand exchange reaction arising out of a coordination function of the silanol groups. This is the first research concerning the coordination function of silanol groups.We also found that the coordination function of the silanol groups also affected the elution of oxine-copper. Therefore, a selection of a suitable HPLC column is crucial in separating oxine-copper with HPLC. We successfully found the most suitable HPLC column with the silanol-detecting probes (tris(2-methyl-8-quinolinolato)iron(III) and tris(2-methyl-8-quinolinolato)gallium(III)). We could develop a sensitive determination method of trace amount of oxine-copper in environmental waters with HPLC. Combing the HPLC method and the JIS method, it was possible to obtain some useful informations about the movement of oxine-copper in the environment.