Highly-functionalization of oxine-copper analysis on the base of coordination ability of residual silanol and environmental analysis of oxine-copper

本研究建立了喹啉铜的分析方法，并应用该方法对喹啉铜在环境中的迁移行为进行了分析。我们采用高效液相色谱法，将硅烷醇基团集中在用作高效液相色谱分离柱的硅胶填料表面，通过研究硅烷醇基团的功能，发现了硅烷醇基团与三（2-甲基-8-羟基喹啉）合铁（III）或三（2-甲基-8-羟基喹啉）合镓（III）之间的相互作用。利用这些结果，我们建立了一种硅胶表面硅醇基团的鉴别方法。我们还澄清了硅烷醇基团和配合物之间的相互作用是由于硅烷醇基团的配位功能所产生的配体交换反应。这是首次对硅醇基团的配位作用进行了研究，并发现硅醇基团的配位作用对8-羟基喹啉-铜的洗脱也有影响。因此，选择合适的高效液相色谱柱是高效液相色谱分离喹啉铜的关键。我们成功地找到了最合适的HPLC柱与硅烷醇检测探针（三（2-甲基-8-喹啉）铁（III）和三（2-甲基-8-喹啉）镓（III））。建立了高效液相色谱法测定环境沃茨中痕量8-羟基喹啉铜的方法。将HPLC法与JIS法相结合，可以获得有关8-羟基喹啉铜在环境中迁移的一些有用信息。

In this research, we investigated the development of an analytical method of oxine-copper and the analysis of the movement of oxine-copper in environment of with the developed method. We used a high-performance liquid chromatography for the purpose and we focused silanol groups on the surface of silica gel packings used as a separation column in HPLC.We found the interaction between the silanol groups and a tris(2-methyl-8-quinolinolato)iron(III) or a tris(2-methyl-8-quinolinolato)gallium(III) through the investigation into a function of the silanol groups. We developed a discrimination method of silanol groups on the surface of silica gel with these results. Also we clarified that the interaction between the silanol groups and the complexes was due to a ligand exchange reaction arising out of a coordination function of the silanol groups. This is the first research concerning the coordination function of silanol groups.We also found that the coordination function of the silanol groups also affected the elution of oxine-copper. Therefore, a selection of a suitable HPLC column is crucial in separating oxine-copper with HPLC. We successfully found the most suitable HPLC column with the silanol-detecting probes (tris(2-methyl-8-quinolinolato)iron(III) and tris(2-methyl-8-quinolinolato)gallium(III)). We could develop a sensitive determination method of trace amount of oxine-copper in environmental waters with HPLC. Combing the HPLC method and the JIS method, it was possible to obtain some useful informations about the movement of oxine-copper in the environment.