Development of Conceputually New Photonics Materials Based on New Luciferins

研究了各种发光生物发光系统的分子基础。发现海洋火虫的生物发光系统表现出典型的荧光素-荧光素酶反应。发现荧光素可以用甲醇提取，荧光素酶可以用热水提取。将甲醇浸提液与水浸提液混合，观察到蓝绿色光的发射情况。用高效液相色谱法从甲醇提取物中分离荧光素，发现荧光素为高极性酸性物质。由于荧光素含量低，目前还不能确定其化学结构。海鱼的生物发光系统也表现出荧光素-荧光素酶反应。经低温（-80℃以下）保存多年，样品仍能保持生物发光能力。为了探索绿磷真菌的生物发光系统，我们从海地巨岛绿磷真菌的子实体中分离出担子孢子。使担子孢子萌发，从中分离出一株结果菌丝体。利用单株子菌丝，成功地进行了子实体的室内培养。我们尝试分离绿光（510nm）附近最大的荧光物质，从而鉴定出lumichrom。在利用萤火虫生物发光系统偶联Aequorea绿色荧光蛋白（GFP）构建生物发光共振能量转移（BRET）系统的过程中，对产生蓝光的萤火虫荧光素类似物进行了分子设计。通过对结构-生物发光活性关系的初步研究，我们成功合成了一种生物发光最大值比天然萤火虫荧光素短的类似物。结合基于生物发光体系的新型光子学材料的开发，我们成功地获得了具有扩展π体系的咪唑吡嗪酮衍生物。少

Molecular basis of light emitting system of various bioluminescent organisms were investigated. The bioluminescence system of the marine sea fire worm Odontosyllis undecimdonta was found to exhibit typical luciferin-luciferase reaction. The luciferin was found to be able to extract with methanol, and the luciferase with hot water. The emition of bluewish green light was observed by mixing the methanol extract and the water extract. The isolation of luciferin from the methanol extract was attempted by using HPLC, and we found that the luciferin to be acidic substance(s) with high polarity. Owing to low quantity of the luciferin content, we could not determine the chemical structure so far.The bioluminescence system of the sea hare Plocamopherus imperialis was found also o exhibited luciferin-luciferase reaction. The sample was found to keep the bioluminescence ability even after storing at low temperature (below-80℃) for years.In connection of exploration of bioluminescence system of th … More e mushroom Mycea chlorophos, we isolated basidiospores from the fruit-body of the mushroom collected from Hatijuo Island. The basidospores were germinated, from which a single strain of the fruiting mycelium was isolated. Using the single strain of the fruiting mycelium, we succeeded laboratory cultivation of fruiting body. We attempted the isolation of fluorescent substance(s) with the maximum around green light(510nm), resulting to the identification of lumichrom.In the course of the construction of Bioluminescence Resonance Energy Transfer (BRET) system utilizing the firefly bioluminescence system coupled with Aequorea green fluorescence protein (GFP), the molecular design of blue light-producing firefly luciferin analogs was performed. After brief studies on the structure-bioluminescence activity relation ship, we succeeded the synthesis of an analog having a bioluminescence maximum shorter than that of native firefly luciferin.In connection with the development of new photonics materials based on bioluminescence systems, we succeeded new imidazopilazinone derivatives with extended π-systems. Less