Molecular Analysis of a novel PDZ protein interacting with orphan glutamate receptorδ2

Delphilin是GluRδ2相互作用蛋白，可能是GluRδ2与多种信号分子的连接蛋白。在本研究中，我们使用表面等离子体共振分析来筛选与Delphilin PDZ结构域相互作用的分子，以阐明其功能。候选分子有：嗜电离性GluRs、代谢性GluRs等相关分子，已知这些分子定位于小脑浦肯野细胞，其c端序列与GluRδ2分子相似。在这些分子中，单羧酸转运体（MCT） 2被发现是与Delphilin PDZ结构域相互作用的候选分子。此外，我们也在探索一些信号分子可能与Delphilin FH1结构域相互作用的可能性。

Delphilin, GluRδ2-interacting protein, may serve to link GluRδ2 with various signaling molecules. In the present study, we used surface plasmon resonance analysis to screen molecules interacting with Delphilin PDZ domain for elucidating its function. Candidate molecules were : ionotropic GluRs, metabotropic GluRs and other related molecules, which are known to be localized in cerebellar Purkinje cells and to have C-terminal sequence similar to that of GluRδ2 molecules. Among these molecules, monocarboxylate transporter (MCT) 2 was found to be a candidate molecule interacting with Delphilin PDZ domain. Furthermore, we are also ezploring the possibility that some signaling molecule(s) may interact with the Delphilin FH1 domain.