Molecular Analysis of Life and Death in Pathogenic Fungi Cryptococcus neoformans : Cell Cycle Cntrol and Interaction between Fungi and Bacteria

病原真菌新生隐球菌生死的分子分析：细胞周期控制以及真菌和细菌之间的相互作用

• 批准号: 20590437
• 负责人: KAWAMOTO Susumu
• 金额: $ 3万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590437/
• 关键词: 真菌; 感染症; 細胞周期; 遺伝子; タンパク質; 国際情報交換; チェコ:ハンガリー; 蛋白質

The G1 Cyclin CnCln1 gene, a key molecule in the cell cycle control in a pathogenic yeast, Cryptococcus neoformans, was cloned, identified and characterized. Interaction between cell cycle control and stress (hypoxia) response was analyzed. Also, localization of triosephosphate isomerase, the molecules responsible for contact-mediated killing of Cryptococcus, on the surface of Staphylococcus was demonstrated by scanning immunoelectron microscopy.

G1 Cyclin CNCLN1基因是致病性酵母中细胞循环控制中的关键分子，即新虫球菌Neoformans，被克隆，鉴定和表征。分析了细胞周期控制与应力（缺氧）反应之间的相互作用。同样，通过扫描免疫电子显微镜证明了三氧磷酸异构酶的定位，即负责接触介导的加密摄氏杀死的分子，该分子在葡萄球菌的表面上进行了定位。

项目成果

Cryptococcus neoformans Wee1 kinase is not essential for growth but has a role in G2-M.

新型隐球菌 Wee1 激酶对于生长不是必需的，但在 G2-M 期发挥作用。

• 发表时间: 2010
• 作者: E.Virtudazo;M.Ohkusu;K.Shimizu;M.Yamaguchi;S.Kawamoto
• 通讯作者: S.Kawamoto

Two key players of cell cycle regulation in Cryptococcus neoformans, Cyclin-dependent kinase (Cdk) and its regulator

新型隐球菌细胞周期调控的两个关键角色，细胞周期蛋白依赖性激酶（Cdk）及其调节因子

• 发表时间: 2010
• 作者: Eweda G;Suzuki D;Nagata T;Tsujimura K;Koide Y;川本進
• 通讯作者: 川本進

The single Cdk1-G1 cyclin of Cryptococcus neoformans is not essential for cell cycle progression but has important roles in the proper commitment to DNA synthesis and bud emergence in this yeast.

新型隐球菌的单个 Cdk1-G1 细胞周期蛋白对于细胞周期进展不是必需的，但在该酵母中的 DNA 合成和芽出现的正确承诺中具有重要作用。

• 发表时间: 2010
• 期刊: FEMS Yeast Research 10(5)
• 作者: Virutudazo EV;Kawamoto S;Ohkusu M;Aoki S;Sipiczki M;Takeo K
• 通讯作者: Takeo K

Difference in physiology and protein expression pattern of capsule thickness variants of Cryptococcus neoformans.

新型隐球菌荚膜厚度变异的生理学和蛋白质表达模式的差异。

• 发表时间: 2009
• 作者: Ohkusu M;Kawamoto S
• 通讯作者: Kawamoto S

Isolation of yeasts from palm tissues damaged by the red palm weevil and their possible effect on the weevil overwintering

• DOI: 10.1007/s10267-009-0029-8
• 发表时间: 2010-05-01
• 期刊: MYCOSCIENCE
• 影响因子: 1.4
• 作者: Abe, Fukiko;Ohkusu, Misako;Hata, Kunihiko
• 通讯作者: Hata, Kunihiko