Molecular mechanism for the extension of neuronal growth cone : role of exocytosis of integrin.

神经元生长锥延伸的分子机制：整合素胞吐作用的作用。

• 批准号: 14580769
• 负责人: TATSUMI Hitoshi
• 金额: $ 2.24万
• 依托单位: nagoya university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580769/
• 关键词: Growth cone; Contact; exocytosis; integrin; fluorescence imaging; near-field light; vesicle trafficking; 伝達物質; エンドサイトシス; 生物物理

Adhesive contact at a growth cone has been reported in a variety of neurons and is thought to be crucial for neurite extension. We have recently demonstrated a close correlation between exocytosis of neurotransmitters and the formation of adhesive contacts at growth cones {Tatsumi & Katayama 1999 4371 /id}. Contact between a cell substrate and a growth cone was also enhanced when excitatory stimulations are applied to growth cones ^<49>. These studies suggest a close interrelation between an adhesive contact and vesicle localization at the growth cones. To examine the relationship between exocytosis and integrin incorporation into cell membrane, cells were stained with FM4-64 (vesicle marker for exocytosis) and an anti-integrin antibody labeled with Alexa488. Time lapse imaging of the stained cell demonstrated that the majority of vesicles contained both integrins and FM4-64. Analysis of the changes of integrins and FM4-64 fluorescence suggests that incorporation of integrins into the surface membrane of the growth cone after exocytosis, resulting in enhancement of contacts to substrate at growth cones.

生长锥处的粘附性接触已在多种神经元中被报道，并且被认为对神经突延伸至关重要。我们最近已经证明了神经递质的胞吐作用与生长锥处粘附接触的形成之间的密切相关性{Tatsumi & Katayama 1999 4371 /id}。当对生长锥施加兴奋性刺激时，细胞基质和生长锥之间的接触也得到增强<49>。这些研究表明，在生长锥的粘附接触和囊泡定位之间的密切相关性。为了检查胞吐作用和整合素掺入细胞膜之间的关系，用FM 4 -64（胞吐作用的囊泡标记物）和用Alexa 488标记的抗整合素抗体对细胞进行染色。染色细胞的时间推移成像表明，大多数囊泡含有整联蛋白和FM 4 -64。整合素和FM 4 -64荧光的变化的分析表明，整合素的掺入到细胞外分泌后的生长锥的表面膜，导致在生长锥与底物的接触增强。

项目成果

Soeda H, Tatsumi H, Kozawa Y, Mishima H, Imai K, Katayama Y: "Functional characterization of calcium channels localized on the growth cones of cultured art dorsal root ganlion cells"Neurosci Lett. 325. 5-8 (2002)

Soeda H、Tatsumi H、Kozawa Y、Mishima H、Imai K、Katayama Y：“培养艺术背根神经节细胞生长锥上钙通道的功能特征”Neurosci Lett。

Tatsumi H: "Bio-imaging using near-field light microscope"Bunkou Kenkyu. 52. 219-224 (2003)

Tatsumi H：“使用近场光学显微镜进行生物成像”Bunkou Kenkyu。

Hoshino, Tatsumi, Nakashima, Sokabe: "In vitro reconstitution of signal transmission from a hair cell to the growth cone of a chick vestibular ganglion cell"Neuroscience. (accepted).

Hoshino、Tatsumi、Nakashima、Sokabe：“从毛细胞到小鸡前庭神经节细胞生长锥的信号传输的体外重建”神经科学。

Sokabe M, Kawakami, K., Hayakawa, K., Naruse, K., Tatsumi H: "Remodeling of endothelial cells depend on mechanical stress : interface between molecule to cell shape."Kekkan igaku. 4. 237-244 (2003)

Sokabe M、Kawakami, K.、Hayakawa, K.、Naruse, K.、Tatsumi H：“内皮细胞的重塑取决于机械应力：分子与细胞形状之间的界面。”Kekkan igaku。

Hoshino, M, Tatsumi H, Sokabe M: "In vitro reconstitution of signal transmission from a hair cell to the growth cone of a chick vestibular ganglion cell"Neuroscience. 120:. 993-1003 (2003)

Hoshino, M、Tatsumi H、Sokabe M：“体外重建从毛细胞到鸡前庭神经节细胞生长锥的信号传输”神经科学。