Development of Cardiac Microangiography system with Spatial resolution of 8 μm using Pulsed Monochromatic Synchrotron Radiation. X-ray

使用脉冲单色同步辐射开发空间分辨率为 8 μm 的心脏微血管造影系统。

• 批准号: 14580844
• 负责人: UMETANI Keiji
• 金额: $ 2.69万
• 依托单位: Japan Synchrotron Radiation Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580844/
• 关键词: Microangiography; Coronary artery; Contrast agent; Synchrotron radiation; Monochromatic X-ray; X-ray SATICON; Vasoactive agent; Vasoconstriction and vasodilatation; 血管拡張反応; 血管収縮反応; 心疾患

A microangiography system with spatial resolution in the micrometer range was developed to depict coronary vascular response in a rat heart under Langendorff perfusion using a high-speed imaging system and a third generation synchrotron radiation source at SPring-8. The imaging system was composed of an X-ray direct-conversion type detector incorporating an X-ray SATICON pickup tube and a high-speed X-ray shutter using a galvanometer based scanner. Microangiographic images were obtained without image blur and stored in a digital frame memory system with a 1024_×1024-pixel, 10-bit format.An X-ray imaging system needs to have high shutter speed (short exposure time) to make sharp and blur-free images of objects. For high-speed imaging, we developed an X-ray shutter system using a moving-coil galvanometer-based optical scanner. An optical scanner is a high-accuracy mirror positioning motor for laser optics. After an optical mirror was removed from the galvanometer-based scanner, a rotatin … More g X-ray chopper was attached to a center shaft of the scanner. Twin tantalum blades on each side of the chopper move in a seesaw-like manner. By about 9° rotation of the chopper, the shutter opens or closes a route for the X-ray beam.The shutter system was located between the monochromator and the object. Since thickness of each tantalum blade is 3 mm, the shutter can be used at X-ray energies of 30-50 keV. The chopper rotates to synchronize timing with synchronous signals of a video camera. The shortest shutter open time was 2 ms. X-ray exposure time is thereby proportional to the shutter open time and each X-ray image is detected in 2 ms by the X-ray direct-conversion type detector.Visualization of vasoconstriction and vasodilatation of coronary arteries with vasoactive agents is a useful research tool for evaluation of coronary vascular reserve. In imaging experiments, the isolated rat heart was mounted on a steel cannula and per fused with oxygenated per fusion fluid. After iodine contrast agent injection into the ascending aorta, small cardiac blood vessels of less than 50 μm diameter were visualized at heart rates of 300-400 per minute under Langendorff per fusion, and vasodilatation in small arteries was visualized in response to vasoactive agents. This synchrotron radiation high-speed imaging system is a useful tool for depicting large and small coronary arteries in fast-moving hearts and for quantifying actual change in their diameter by vasoactive agents. Less

利用高速成像系统和SPring-8的第三代同步辐射光源，开发了一种空间分辨率在微米范围内的微血管造影系统，用于描述Langendorff灌注下大鼠心脏的冠状动脉血管反应。成像系统由X射线直接转换型探测器组成，该探测器包括X射线SATICON拾取管和使用基于检流计的扫描器的高速X射线快门。微血管造影图像无图像模糊，并以1024 × 1024像素，10位格式存储在数字帧存储系统中。X射线成像系统需要具有高快门速度（短曝光时间）以使物体的图像清晰且无模糊。对于高速成像，我们开发了一种X射线快门系统，使用基于动圈式检流计的光学扫描仪。光学扫描器是用于激光光学的高精度镜面定位电机。从基于检流计的扫描仪中移除光学镜后，旋转 ...更多信息 g将X射线斩波器连接到扫描仪的中心轴。双钽刀片在每一方的斩波器移动在一个跷跷板般的方式。遮光器旋转约9 °，遮光器打开或关闭X射线束的路径。遮光器系统位于单色器和物体之间。由于每个钽叶片的厚度为3 mm，因此快门可以在30 - 50 keV的X射线能量下使用。斩波器旋转以使定时与摄像机的同步信号同步。最短的快门打开时间为2ms。因此，X射线曝光时间与快门打开时间成正比，并且通过X射线直接转换型检测器在2ms内检测到每个X射线图像。使用血管活性剂的冠状动脉的血管收缩和血管舒张的可视化是用于评价冠状动脉血管储备的有用的研究工具。在成像实验中，将离体大鼠心脏固定在钢套管上，并用充氧的灌注融合液灌注融合。在升主动脉注射碘造影剂后，在Langendorff每融合下以300 - 400次/分钟的心率可视化直径小于50 μ m的小心脏血管，并可视化小动脉中对血管活性剂的反应性血管舒张。这种同步辐射高速成像系统是一种有用的工具，用于描绘快速运动的心脏中的大小冠状动脉，并用于量化血管活性剂对其直径的实际变化。少

项目成果

梅谷啓二, 福島和人, 杉村和朗: "X線サチコンでの放射光血管造影による微小血管拡張反応の観察"映像情報メディア学会誌. 58・3. 344-351 (2004)

Keiji Umetani、Kazuto Fukushima、Kazuo Sugimura：“X 射线 sachicon 中同步辐射血管造影的微血管扩张反应观察”图像信息和电视工程师学会杂志 58・3（2004 年）。

梅谷啓二, 福島和人, 魚谷健祐, 鶴崎正勝, 杉村和朗: "X線サチコンを使ったパルスX線での心臓血管撮影"情報メディア学会技術報告. 27・58. 21-24 (2003)

Keiji Umetani、Kazuto Fukushima、Kensuke Uotani、Masakatsu Tsurusaki、Kazuo Sugimura：“使用 X 射线 Sachicon 进行脉冲 X 射线的心血管成像”日本信息和媒体工程师技术报告 27・58。

Observation of microvasculatures in athymic nude rat transplanted tumor using synchrotron radiation microangiography system.

• DOI: 10.1016/j.acra.2004.06.003
• 发表时间: 2004-09
• 期刊: Academic radiology
• 影响因子: 4.8
• 作者: R. Tokiya;K. Umetani;S. Imai;T. Yamashita;J. Hiratsuka;Y. Imajo

X-ray SATICON Based Direct-Sensing Detector for Small-Field Angiographic Imaging

基于 X 射线 SATICON 的直接感应探测器，用于小视场血管造影成像

• 发表时间: 2003
• 期刊: Proc.Joint Symposium on Bio-Sensing and Bio-Imaging
• 作者: Keiji Umetani;Takenori Yamashita;Nobunao Maehara et al.
• 通讯作者: Nobunao Maehara et al.

Keiji Umetani, Kazuhito Fukushima, Masakatsu Tsurusaki et al.: "Microangiography in isolated perfused rat organs for evaluation of vascular response"Proc.Symposium on Towards Clinical Application of Synchrotron Radiation, SPring-8,September 2002. 36-41 (2

Keiji Umetani、Kazuhito Fukushima、Masakatsu Tsurusaki 等人：“用于评估血管反应的离体灌注大鼠器官中的显微血管造影”Proc.Symposium on Towards Clinical Application of Synchrotron Radiation，SPring-8，2002 年 9 月。 36-41 (2