Analysis of spatio-temporarl mechanisms on outer plexiform layer in retina by the multichannel microprobe electrodes array chip

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We have developed multichannel microprobe electrode array chips for recording neural responses to light from retina, and mathematical model of retinal neurons to analyze retinal mechanisms involved in the recorded light-induced responses.Prototypes of the microprobe electrode array(MEA) chip have been fabricated on Si(111) substrates by selective Vapor-Liquid-Solid(VLS) growth method. The prototype MEA could detect light responses whose characteristics to a white flash and sensitivities to monochromatic stimulus were consistent with photopic electoretinogram(ERG). We also demonstrated that the changes of localized ERG responses were obtained with stimulus of slit light by the MEA.In order to analyze inner retinal mechanisms involved in the measured ERG responses, we have developed models of retinal neuron and the retinal neural circuit by physiological engineering technique. The models of horizontal cell and bipolar cells which is closely related to form b-wave in ERG responses were described mathematically by their ionic mechanisms. It is allow us to analyze the relationship between light responses and the ionic mechanisms in their neuron.These results suggest that the developed MEA chip is applicable to retinal neural recordings as a new measurement tool, and the models of retinal neuron would play a role to understand visual information preprocessing of retina.