Pathophysiology of the treatment of transient cerebral ischemia

短暂性脑缺血治疗的病理生理学

• 批准号: 15500230
• 负责人: KUROIWA Toshihiko
• 金额: $ 2.11万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15500230/
• 关键词: Cerebral ischemia; infarction; sdelective neuronal death; neurological deficit; magnetic resonance imaging; 血栓溶解; 微小血栓; E-Selection

We have evaluated postischemic neurological and cognitive dysfunction after mild focal cerebral ischemia in Mongolian gerbil. Mongolian gerbils underwent the left common carotid artery occlusion (CCAO) for 10, 15, or 20 min. A battery of neurological tests including T maze test, bilateral asymmetry test, open field test, elevated body swing test and methamphetamine (MP)-induced rotation test was performed to evaluate the neurological and cognitive dysfunction including f extrapyramidal motor dysfunction.1.Postischemic motor and somatosensory functions were significantly correlated with regional infarction volumes in the corresponding cortical regions. In gerbils, visual abnormality could be independently detected by the T-maze test. Such regional analyses of ischemic lesions would be useful for investigating the functional outcomes of stroke therapy.2.Biased rotation behavior is a sensitive parameter of the extent of striatal injury after focal cerebral ischemia provided the lesion is … More not extended to the ipsilateral cortex. UP-induced rotation in rodents probably coordinates with the extrapyramidal motor dysfunction after striatal infarction in patients with vascular Parkinsonism.3.The total infarction volumein the neuronal stem cell -grafted animals was significantly lower than that in controls. Approximately 8% of the grafted stem cell urvived, mainly in areas of selective neuronal death, and were costained with antibodies against neuronal nuclei antibody, microtubule associated protein, glial fibrillary acidic protein. Synaptic structures between stem cell derived neurons and host neurons were bserved. Furthermore, gradual improvement of neurologic functions was observed clearly in the stem cell grafted animals, compared to that in controls. Human neuronal stem cell remarkably improved neurologic functions after focal ischemia in the Mongolian gerbil, and maintained their abilities to migrate around the infarction, differentiate into mature neurons, and form synapses with host neuronal circuits. Less

我们评估了蒙古沙土鼠轻度局灶性脑缺血后的神经和认知功能障碍。蒙古沙土鼠左侧颈总动脉结扎(CCAO)10、15、20min。采用T迷宫试验、双侧不对称试验、开野试验、高位摆动试验和甲基苯丙胺(MP)诱导的旋转试验等一系列神经学检查，评价脑缺血后大鼠的神经和认知功能障碍，包括锥体外系运动功能障碍。1.脑缺血后运动和躯体感觉功能与相应皮质区域梗死灶体积显著相关。在沙土鼠中，视觉异常可以通过T迷宫测试独立检测到。这种对缺血损伤的区域性分析将有助于研究中风治疗的功能结果。2.偏向旋转行为是局灶性脑缺血后纹状体损伤程度的敏感参数，前提是损伤是…更多的不是延伸到同侧皮质。血管性帕金森病患者纹状体梗塞后锥体外系运动功能障碍可能与啮齿类动物UP诱导的旋转相一致。3.神经干细胞移植动物的总梗塞体积明显小于对照组。大约8%的移植干细胞存活，主要分布在选择性神经元死亡的区域，并与抗神经元核抗体、微管相关蛋白、胶质纤维酸性蛋白的抗体共染色。观察了干细胞来源神经元与宿主神经元之间的突触结构。此外，与对照组相比，干细胞移植组动物的神经功能明显改善。人神经干细胞显著改善蒙古沙土鼠局灶性缺血后的神经功能，并维持其在梗塞周围迁移、分化为成熟神经元以及与宿主神经元回路形成突触的能力。较少

项目成果

スナネズミ一過性一側総頚動脈閉塞局所脳虚血モデルにおける2種類のhyperactivityと対応する虚血性病変分布

沙鼠短暂性单侧颈总动脉闭塞局灶性脑缺血模型中两种类型的多动及相应的缺血病灶分布

• 发表时间: 2004
• 期刊: 脳循環代謝:,2004 16
• 作者: 勝亦憲子;黒岩俊彦;他
• 通讯作者: 他

Ishibashi S, Kuroiwa T, Endo S, Okeda R, Mizusawa H.: "Neurological dysfunctions versus regional infarction volume after focal ischemia in Mongolian gerbils."Stroke. 34. 1501-1506 (2003)

Ishibashi S、Kuroiwa T、Endo S、Okeda R、Mizusawa H.：“蒙古沙鼠局部缺血后的神经功能障碍与局部梗塞体积。”中风。

Yamada I, Kuroiwa T, Endo S, Miyasaka N.: "Temporal evolution of apparent diffusion coefficient and T2 value following transient focal cerebral ischemia in gerbils."Acta Neurochir Suppl. 86. 147-151 (2003)

Yamada I、Kuroiwa T、Endo S、Miyasaka N.：“沙鼠短暂局灶性脑缺血后表观扩散系数和 T2 值的时间演变。”Acta Neurochir Suppl。

PET imaging of adenosine A(1) receptors with (11)C-MPDX as an indicator of severe cerebral ischemic insult.

使用 (11)C-MPDX 对腺苷 A(1) 受体进行 PET 成像作为严重脑缺血损伤的指标。

• 发表时间: 2003
• 期刊: J Nucl Med. 44
• 作者: Nariai T;Shimada Y;Ishiwata K;Nagaoka T;Shimada J;Kuroiwa T;Ono K;Ohno K;Hirakawa K;Senda M.
• 通讯作者: Senda M.

Ishibashi S, Kuroiwa T, Endo S, Okeda R, Mizusawa H.: "A comparison of long-term neulogical symptoms after two different focal ischemic models in Mongolian gerbils."Acta Neurochie Suppl. 86. 159-162 (2003)

Ishibashi S、Kuroiwa T、Endo S、Okeda R、Mizusawa H.：“蒙古沙鼠两种不同局部缺血模型后长期神经症状的比较。”Acta Neurochie Suppl。