Quantitative spectral analysis of 5-ALA derived porphyrin fluorescence and auto-fluorescence for improving PDD

点击翻译按钮获取中文摘要

As for the photodynamic diagnosis using 5-ALA, the judgments of fluorescence intensity is not quantitative but subjective by naked eyes. Therefore, the precision of diagnosis is still limited. In this study, we newly developed the quantitative spectral analysis method for fluorescence derived form 5-ALA induced porphyrin and brain tissue using spectroradiometer. We applied this methods for clinical samples and could quantitatively measure fluorescence as spectral radiance, which SI unit is W^*sr^<-1*>m^<-2*>nm^<-1>. We confirmed the relationship between fluorescence radiance and clinical properties, such as ordinal judgment of fluorescent intensity and enzyme level of porphyrin synthesis.