Low performance of fatigued skeletal muscle accompanies large difference in the state of water : test of hypothesis.

疲劳的骨骼肌的低性能伴随着水状态的巨大差异:假设检验。

基本信息

  • 批准号:
    15500459
  • 负责人:
  • 金额:
    $ 2.11万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2004
  • 项目状态:
    已结题

项目摘要

Repetitive contraction of frog muscle elicited by tetanic nerve stimuli induced general retardation of transverse relaxation process of muscle water observed with NMR. Similar change was observed in lumbrical muscle of rat stimulated directly. Therefore, similar change is expected to accompany muscle fatigue in humans. To elucidate the entity of this change in water state, we performed experiments on myofibril suspension. Myofibrils affected water molecules far from its surface. The fraction of this suppressed water increased with myofibril concentration in a temperature dependent manner. The dependency indicated the interaction between water and muscle is the hydrophobic interaction. The interaction also affected physical property of water affecting the sedimentation limit for myofibrils. In the model muscle fiber of fatigued muscle induced by BTS and BDM, there is a fraction of stiffness that is not linked with contractile force development. This stiffness in muscle may be related with the fatigue-induced change in water state. As for the intramuscular water components, we assigned subcellular localization of water components distinguished by the transverse relaxation. To mimic the water milieu in muscle, we succeeded to develop hydrocarbon solution of low to intermediate molecular weight. Combined with the molecular dynamics simulation, we could estimate the molecular events accompanying fatiguing process of skeletal muscle. From these results, we proposed a sensitive method to detect fatiguing process in human skeletal muscle. That is, eliminating slowly relaxing part of the NMR signals from skeletal muscle, one can depict intracellular water components as a density map. Fatigue-induced retardation of the transverse relaxation process is considered to increase CPMG visible water as fatiguing process proceeds.
用核磁共振技术观察到强直神经刺激引起的蛙肌重复收缩引起肌水横向松弛过程的普遍延迟。直接刺激大鼠蚓状肌也观察到类似的变化。因此,预计类似的变化伴随着人类的肌肉疲劳。为了阐明这种水状态变化的实体,我们对肌原纤维悬浮液进行了实验。肌原纤维影响远离其表面的水分子。这种抑制水的分数增加与肌原纤维浓度在一个温度依赖性的方式。这种依赖性表明水与肌肉之间的相互作用是疏水相互作用。这种相互作用还影响水的物理性质,从而影响肌原纤维的沉降极限。在BTS和BDM诱导的疲劳肌肉模型肌纤维中,有一部分刚度与收缩力的发展无关。肌肉的这种僵硬可能与疲劳引起的水状态变化有关。至于肌内水成分,我们分配的横向弛豫区分水成分的亚细胞定位。为了模拟肌肉中的水环境,我们成功地开发了低至中等分子量的烃溶液。结合分子动力学模拟,我们可以估计骨骼肌疲劳过程中的分子事件。根据这些结果,我们提出了一个敏感的方法来检测人体骨骼肌疲劳过程。也就是说,消除来自骨骼肌的缓慢弛豫的部分核磁共振信号,可以将细胞内水成分描绘为密度图。随着疲劳过程的进行,横向弛豫过程的延迟被认为是增加CPMG可见水的原因。

项目成果

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TAKEMORI Shigeru其他文献

TAKEMORI Shigeru的其他文献

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{{ truncateString('TAKEMORI Shigeru', 18)}}的其他基金

Stretch of muscle sarcomere induces protrusion of myosinheads toward actin so as to facilitate formation of contractile cross-bridges.
肌肉肌节的拉伸诱导肌球蛋白头向肌动蛋白突出,从而促进收缩性横桥的形成。
  • 批准号:
    22616008
  • 财政年份:
    2010
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A Study of Thermo-chemo-embolization Using Dextran Magnetite Incorporated Liposomes
右旋糖酐磁铁矿脂质体热化疗栓塞的研究
  • 批准号:
    09671289
  • 财政年份:
    1997
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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