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The influence of nutrition, physical activity and endocrine on the bone mass of the female college students dieting to obtain a slim body-Experiments on humans and osteoblasts

营养、体力活动和内分泌对节食减肥女大学生骨量的影响——人体和成骨细胞实验

基本信息

批准号：
15500566
负责人：
KAWANO Setsuko
金额：
$ 2.37万
依托单位：
College of Nagoya Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

Experiments on humans : We investigated how dieting, menstrual cycle and body fat rate would influence a change in the bone mass in 401 female college students with an informed consent. There were significant positive relationships between the diet-experience and the bone mass (p<0.01), and the body fat rate and the bone mass (p<0.01), contrary to our assumption. These results suggest that individuals dieting to obtain a slim body did not necessarily lose their body weight and higher pressure to the bone due to their greater weight positively influenced the bone mass. The menstrual cycle had no relationship to the bone mass. In addition, we investigated the estrogen receptor (ER) polymorphisms among 166 students who agreed to this experiment. We carried out RFLP (Restriction Fragment Length Polymorphism) analysis of ER alpha-intron I using Pvu II and Xba I, after obtaining their DNA. Students having menstrual cycle problems (48.3%) fell to the ppxx (n=60) group, and this group's bone s … More tiffness (77.0) showed the second lowest value, next to that of the PPXX group (89.0, n=4). Physical activity showed a positive influence on the bone mass (p<0.05) in the Ppxx, PpXx and ppxx groups, suggesting that the effects of physical activity differ among the genetic traits. These results suggest that, especially among the Ppxx, PpXx and ppxx type individuals, a modest effort to keep a proper body weight and daily practice of light physical activity may be effective in preventing the loss of bone mass and, consequently, in preventing an increase in the number of the bedridden among the aged population.Experiments on osteoblasts : We investigated the effects of estrogen (1 nM) or testosterone (100 nM) on the expression of vitamin D3 receptor mRNA in osteoblastic ROS17/2.8 cells. Treatment of the cells with either one of the hormones for 24 hours increased the receptor expression. However, the testosterone effect was suppressed by a non-steroidal reversible aromatase inhibitor, letrozole. These results strongly suggest that aromatase present in osteoblasts contributes to the synthesis of estrogen in these cells. Less

人类实验：我们调查了401名女大学生在知情同意的情况下，节食、月经周期和体脂率对骨量变化的影响。膳食经验与骨量呈显著正相关（p<0.01），体脂率与骨量呈显著正相关（p<0.01），与我们的假设相反。这些结果表明，通过节食来获得苗条身材的人不一定会失去体重，并且由于体重增加而对骨骼产生的更高压力会对骨量产生积极影响。月经周期与骨量无关。此外，我们调查了166名同意本实验的学生的雌激素受体（ER）的多态性。在获得它们的DNA后，我们使用Pvu II和Xba I对ER α-内含子I进行RFLP（限制性片段长度多态性）分析。有月经周期问题的学生（48.3%）属于ppxx组（n=60），该组的骨 ...更多信息僵硬度（77.0）仅次于PPXX组（89.0，n=4）。在Ppxx、PpXx和ppxx组中，体力活动对骨量有积极影响（p<0.05），表明体力活动的影响在遗传性状之间存在差异。这些结果表明，特别是在Ppxx、PpXx和ppxx型个体中，适度努力保持适当的体重和每天进行轻体力活动可能有效地防止骨量丢失，从而防止老年人群中卧床不起人数的增加。我们研究了雌激素（1 nM）或睾酮（100 nM）对成骨细胞ROS 17/2.8细胞中维生素D3受体mRNA表达的影响。用任何一种激素处理细胞24小时增加了受体表达。然而，睾酮的作用被抑制的非甾体可逆芳香酶抑制剂，来曲唑。这些结果有力地表明，成骨细胞中存在的芳香化酶有助于这些细胞中雌激素的合成。少