In vivo detection and identification of free radicals

（1）7-羧基庚基、戊基、7-羧基二羟基庚基、1，5-二羟基戊基、8-羧基-1-羟基辛基、7-羧基-1-羟基庚基、1-羟基戊基，利用HPLC-ESR-MS技术在亚油酸氧化产物中检测到了7-羧基庚基自由基和1-羟基己基自由基。本发明的方法通过与13-羟基过氧-9，11-十八碳二烯酸与亚铁离子反应中的机制不同的机制，将羟基过氧-9，11-十八碳二烯酸与几种血红素蛋白反应。（3）在1，2-二亚油酰基-sn-甘油基-3-磷酸胆碱（DLPC）与大豆脂氧合酶、DLPC与亚铁离子氧化反应以及DLPC过氧化氢与亚铁离子反应的混合物中检测到戊基自由基。(4)A木犀草素-7-G（luteolin-7-G）能抑制13-HPODE（13-hydroperoxy-9，11-octadecadienoic acid，13-HPODE）与Fe（II）反应生成戊基和7-羧基庚基自由基。毛地黄黄酮-7-G的抑制作用部分是通过螯合铁离子来实现的。（5）乙醚染毒大鼠血清与亚铁离子反应混合物中检出的自由基为1-乙氧基乙基自由基。（6）在抗坏血酸/EDTA/离子体系中检测到大鼠脑匀浆中的乙基自由基。（7）微透析法检测再灌注后大鼠脑组织自由基的变化。

(1)7-Carboxyheptyl radical, pentyl radical, 7-carboxyldihydroxyheptyl radical, 1,5-dihydroxypentyl radical, 8-carboxy-1-hydroxyoctyl radical, 7-carboxy-1-hydroxyheptyl radical, 1-hydroxypentyl radical, and 1-hydroxyhexyl radical were detected and identified in the reaction mixture of oxidized linoleic acid with ferrous ions using HPLC-ESR-MS. (2)7-Carboxyheptyl radical is formed in the reaction mixtures of 13-hydroperoxy-9,11-octadecadienoic acid with several hemoproteins through a mechanism different from the one in the reaction of 13-hydroperoxy-9,11-octadecadienoic acid with ferrous ions. (3)Pentyl radicals were detected and identified in the reaction mixtures of 1,2-dilinoleoyl-sn-glycero-3-phosphochline (DLPC) with soybean lipoxygenase, oxidized DLPC with ferrous ions, and DLPC hydroperoxide with ferrous ions. (4)A flavone glucoside, luteolin-7-O-glucoside (luteolin-7-G) inhibited the formation of pentyl and 7-carboxyheptyl radicals in the reaction of 13-hydroperoxy-9,11-octadecadienoic acid (13-HPODE) with iron (II) ions. The inhibitory effects of luteolin-7-G occur partly through the chelation of iron ions. (5)The radicals detected in the reaction mixture of ferrous ions with the serums from rats exposed to diethyl ether were identified as 1-ethoxyethyl radicals. (6)Ethyl radicals were detected in the reaction mixture of rat brain homognate with ascorbate/EDTA/ions system. (7)Radicals were detected in rat brain after reperfusion using microdialysis.