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Study on the molecular conformational equilibrium of butane for the development of an experimental model system of the hydrophobic effect

丁烷分子构象平衡研究，用于疏水效应实验模型系统的开发

基本信息

批准号：
15550020
负责人：
KATO Minoru
金额：
$ 1.41万
依托单位：
Ritsumeikan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

It is well-known that the hydrophobic effect is a critical factor for structural stability of proteins. The purpose of this project is to develop an experimental model for the hydrophobic effect. As a model, we investigated conformational equilibrium of simple chain molecules by using Raman spectroscopy. The most simple and ideal model is the trans gauche equilibrium of butane. We first target this system in aqueous solution. However, it was difficult to observe the equilibrium by Raman spectroscopy due to the low solubility of butane into water. During this project term, we succeed to observe qualitatively the equilibrium, indicating that the relative population of the gauche conformer increased compared with the organic solvent systems. The further experiments for the quantitative analysis are in progress. As another targets we investigated trans-gauche/gauche-gauche equilibrium of diethyl disulfide, which is a model for disulfide bonds for proteins. We elucidated the effect of temperature and pressure on the equilibrium, and discuss the phenomena in comparison with the conformational change of disulfide of a protein when it unfolds. The other research results were obtained for a simple peptide system. We target a simple alanine-rich peptide as a model molecule, which is suitable to observe the helix-coil equilibrium in water. Effect of pressure on the equilibrium showed that the relative population of helix form increases with increasing pressure. This behavior is apparently inconsistent with pressure-induced unfolding of protein. Further projects for elucidating the inconsistency are in progress.

众所周知，疏水效应是影响蛋白质结构稳定性的关键因素。本项目旨在建立疏水效应的实验模型。作为模型，我们用拉曼光谱研究了单链分子的构象平衡。最简单、最理想的模型是丁烷的反式间扭平衡。我们首先在水溶液中定位这个系统。然而，由于丁烷在水中的溶解度较低，用拉曼光谱很难观察到平衡。在本项目期间，我们成功地定性地观察了平衡，表明与有机溶剂体系相比，间扭构象的相对居数增加了。进一步的定量分析实验正在进行中。作为另一个目标，我们研究了二乙基二硫化物的反式-间扭式/间扭式-间扭式平衡，这是蛋白质二硫化物键的模型。我们阐明了温度和压力对平衡的影响，并与蛋白质展开时二硫化物的构象变化进行了比较。其他研究结果是在一个简单的肽体系中得到的。我们选择了一个简单的富含丙氨酸的肽作为模型分子，它适合于观察水中的螺旋-线圈平衡。压力对平衡的影响表明，螺旋型的相对数量随着压力的增加而增加。这种行为显然与压力诱导的蛋白质展开不一致。进一步阐明不一致性的项目正在进行中。

项目成果

期刊论文数量（26）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

FT-IR Spectroscopic Study on Conformational Equilibria of [Leu]^5-Enkephalin in DMSO and ^2H_2O Solutions

DMSO和^2H_2O溶液中[Leu]^5-脑啡肽构象平衡的红外光谱研究

DOI：
发表时间：
2004
期刊：
J.Mol.Liq. 119(1-3)
影响因子：
0
作者：
T.Imai;T.Takekiyo;A.Kovalenko;F.Hiata;M.Kato;Y.Taniguchi;Takahiro Takekiyo et al.;Takahiro Takekiyo et al.;Hideto Isogai et al.;Takahiro Takekiyo et al.;Takahiro Takekiyo et al.
通讯作者：
Takahiro Takekiyo et al.

Raman Study of Hydration Effect on Conformational Equilibria of Haloacetones

卤代丙酮水合效应对构象平衡的拉曼研究

DOI：
发表时间：
2003
期刊：
Bull.Chem.Soc.Jpn 76
影响因子：
0
作者：
T.Imai;T.Takekiyo;A.Kovalenko;F.Hiata;M.Kato;Y.Taniguchi;Takahiro Takekiyo et al.;Takahiro Takekiyo et al.;Hideto Isogai et al.;Takahiro Takekiyo et al.;Takahiro Takekiyo et al.;Yosuke Shiratori et al.
通讯作者：
Yosuke Shiratori et al.

Y.Shiratori, Y.Nanba, M.Kato, Y.Taniguchi: "Raman Study of Hydration Effect on Conformational Equilibria of Haloacetones"Bull.Chem.Soc.Jpn. 76. 501-507 (2003)

Y.Shiratori、Y.Nanba、M.Kato、Y.Taniguchi：“卤丙酮构象平衡水合效应的拉曼研究”Bull.Chem.Soc.Jpn。