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Development of a quick in -vivo sensing system based on fiber-optics for cancer diagnostics at very early stage

开发基于光纤的快速体内传感系统，用于早期癌症诊断

基本信息

批准号：
15560362
负责人：
SASAKI Issei
金额：
$ 2.37万
依托单位：
Hokkaido Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15560362/
关键词：
optical fiber sensor optical-bio-sensor cancer diagnositcs cytochrome P450 cancer initiation enzyme P450 EROD cytochrome P450 ethoxyresorufin

项目摘要

It is well know that at the initial stage of cancer development, some particular types of enzyme such as cytochrome P450 1A often act an important role with carcinogens to produce DNA-carcinogen adducts, as initiation of cancer development. In fact, it is understood that the induction itself of such type of enzymes maybe a remarkable indication of higher cancer risk condition. We have thus proposed anew method of cancer diagnostics based on the information of enzyme activities. It maybe invaluable on medical point of the view if cancer diagnostics can be done at very early stage before arising cancer cells.In the research term of 2003 -2004 of the grant, we have paid our efforts to the development of a fiber-optic sensing system in order to realize the new method of cancer diagnostics. As the result of the work, a quick (short consumption time for diagnostic treatment), in-vivo (directly applicable to human skim) system has been successfully completed. The system uses fluorescent species caused by the enzyme reaction to detect the object enzyme activities by means of optical technique using a pair of optical fibers; one is for delivering excitation light source and the other for guiding signal light to a highly sensitive photo-detector. The higher activity of the enzymes reveals the steeper slope in the curve of luminescent power increase and thus it can be evaluate from the behavior of the fluorescent power at the fiber sensor output.Because of its smallness in diameter and flexibility, a plastic optical fiber was employed as the fiber sensor and thus it allows us to access to anywhere in a body. We found, however, that optimum condition in the space between sensor head and the object to be tested exists at Immseparation.In conclusions, non-contact operation of the new cancer diagnostic method is now technically possible and we have a plan to continue the research for practical use of the fiber optic sensor system to real medial cancer diagnostics.

众所周知，在癌症发生发展的初始阶段，某些特定类型的酶如细胞色素P450 1A通常与致癌物一起发挥重要作用，产生DNA-致癌物加合物，作为癌症发展的起始。事实上，可以理解的是，这种类型的酶的诱导本身可能是较高癌症风险状况的显著指示。因此，我们提出了一种基于酶活性信息的癌症诊断新方法。在2003 - 2004年的研究期内，我们致力于开发一种光纤传感系统，以实现癌症诊断的新方法。作为这项工作的结果，一个快速（诊断治疗消耗时间短），在体内（直接适用于人体脱脂）系统已成功完成。该系统利用酶反应产生的荧光物质，通过一对光纤的光学技术检测目标酶的活性，一根光纤用于传输激发光源，另一根光纤用于将信号光引导到高灵敏度的光电探测器。酶的活性越高，表明发光功率增加曲线的斜率越陡，因此可以从光纤传感器输出的荧光功率的行为来评估。由于其直径小和灵活性，塑料光纤被用作光纤传感器，因此它允许我们进入身体的任何地方。然而，我们发现，在Immseparation.In结论，非接触式操作的新的癌症诊断方法之间的空间中的传感器头和被测对象存在的最佳条件，现在在技术上是可能的，我们有一个计划，继续研究的实际使用的光纤传感器系统，以真实的医学癌症诊断。