Optical fiber sensor system development and its application to research of cancer development

在本研究中，北海道工业大学与北海道大学兽医研究生班合作，集中开发了一种用于检测活体酶的新型光纤传感器技术。光纤传感器及其驱动系统的系统设计与制造，主要在北海道工业大学。它在北海道大学癌症发展医学研究中的应用。经过2年的连续工作，以1的结果成功构建了新的酶检测系统（Ver.1）。当使用该设备观察到给病人开苯并吡喃（一种常见的致癌物）的大鼠肝脏中发现CYP1A1时，它成功地检测到了酶活性在一天后增加的情况，以及致癌物浓度的差异等，灵敏度高。此外，在控制条件下，极少量的酶反应也能被捕捉到。该设备可实现酶反应的实时跟踪，对酶反应机理细节的分析研究具有重要意义。获得了将紫外线照射大鼠耳皮肤作为2、治愈率等被认为致癌因素时，基于CYP1A1的情况定量进行癌变概率测量评价的前景。此外，对1开发的系统进行了改进，并成功地开发出了性能进一步提高约100倍的系统。一种可能性是，关于酶活性的知识可以在癌症症状出现之前利用这些结果来诊断癌症，这意味着该系统可以应用于癌症诊断设备的方向。

In this research, a new optical fiber sensor technology has been intensively developed for detecting enzyme in living body under the collaboration of Hokkaido Institute of Technology and the Graduate Course of Veterinary Medicine, Hokkaido University for the two major programs.1. System design and fabrications of optical fiber sensor and its driving system, mainly at Hokkaido Institute of Technology.2. Its application to medical research of cancer development at Hokkaido University.After 2-year successive work, it succeeded in building the new enzyme detection system (Ver.1) as a result of 1. When discovery of CYP1A1 in the liver of the rat which prescribed for the patient benzopyren, a popular carcinogen, was observed using this equipment, it succeeded in detecting the situation that enzyme activity increases the day later on, the difference by the concentration of a carcinogen, etc., in high sensitivity. Moreover, also in control, the reaction by very small quantity enzyme was also able to be caught. With this equipment, tracing in real-time enzyme reaction can be possible, and it may be invaluable to the analytical researches of details about the enzyme reaction mechanism.The prospect that the measurement evaluation of the probability of cancer based on the situation of CYP1A1 when giving ultraviolet ray exposure to the ear skin of a rat as a factor considered to cause cancer as a result of 2, the rate of recovery, etc. could be carried out quantitatively was acquired.Furthermore, improvement was added to the system developed by 1, and it succeeded in the development of a system improved further approximately 100 times better performance. A possibility of saying that the knowledge about activity of enzyme can use these results for diagnosis of cancer before development of symptoms of cancer is shown, and it means that this system is applicable in a direction called cancer diagnostic equipment.