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Optical fiber sensor system development and its application to research of cancer development

光纤传感器系统的开发及其在癌症发展研究中的应用

基本信息

批准号：
11650431
负责人：
SASAKI Issei
金额：
$ 2.3万
依托单位：
Hokkaido Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11650431/
关键词：
Metabolism enzyme Cytochrome P450 Optical fiber sensor Carcinogen cancer mechanism Plastic optical fiber CYP1A1 発ガン酵素蛍光分折法発ガン機構

项目摘要

In this research, a new optical fiber sensor technology has been intensively developed for detecting enzyme in living body under the collaboration of Hokkaido Institute of Technology and the Graduate Course of Veterinary Medicine, Hokkaido University for the two major programs.1. System design and fabrications of optical fiber sensor and its driving system, mainly at Hokkaido Institute of Technology.2. Its application to medical research of cancer development at Hokkaido University.After 2-year successive work, it succeeded in building the new enzyme detection system (Ver.1) as a result of 1. When discovery of CYP1A1 in the liver of the rat which prescribed for the patient benzopyren, a popular carcinogen, was observed using this equipment, it succeeded in detecting the situation that enzyme activity increases the day later on, the difference by the concentration of a carcinogen, etc., in high sensitivity. Moreover, also in control, the reaction by very small quantity enzyme was also able to be caught. With this equipment, tracing in real-time enzyme reaction can be possible, and it may be invaluable to the analytical researches of details about the enzyme reaction mechanism.The prospect that the measurement evaluation of the probability of cancer based on the situation of CYP1A1 when giving ultraviolet ray exposure to the ear skin of a rat as a factor considered to cause cancer as a result of 2, the rate of recovery, etc. could be carried out quantitatively was acquired.Furthermore, improvement was added to the system developed by 1, and it succeeded in the development of a system improved further approximately 100 times better performance. A possibility of saying that the knowledge about activity of enzyme can use these results for diagnosis of cancer before development of symptoms of cancer is shown, and it means that this system is applicable in a direction called cancer diagnostic equipment.

本研究在北海道工业大学和北海道大学兽医研究生院两大项目的合作下，密集开发了一种新型的检测活体酶的光纤传感器技术。主要在北海道工业大学进行光纤传感器及其驱动系统的系统设计和制造。它在北海道大学癌症发生的医学研究中的应用。经过两年的连续工作，它成功地建立了新的酶检测系统(版本1)，结果是1。当使用该设备观察到为患者开出的常见致癌物苯并吡喃的大鼠肝脏中发现了CYP1A1时，它成功地检测到酶活性第二天升高的情况，以及致癌物浓度的差异等，具有高灵敏度。此外，同样在控制下，用极少量的酶进行的反应也能够被捕获。利用该设备，可以实现实时酶反应的示踪，对于酶反应机理细节的分析研究具有不可估量的价值。获得了基于紫外线照射大鼠耳部皮肤时细胞色素P1A1的情况作为致癌因素2、恢复率等的致癌概率测量评估的前景。此外，对1开发的系统进行了改进，并成功地开发出性能进一步提高约100倍的系统。一种可能性是，关于酶活性的知识可以在癌症症状出现之前使用这些结果来诊断癌症，这意味着这个系统适用于一个称为癌症诊断设备的方向。