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Production of usable materials from sugar beet pulp using bioreactor with equipment for electrical pre-treatment of substrate.

使用生物反应器和底物电预处理设备从甜菜浆生产可用材料。

基本信息

批准号：
15560712
负责人：
TOKUDA Hiroharu
金额：
$ 1.28万
依托单位：
Junior college of Tokyo University of Agriculture
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

1.Screening of fungi having high hydrolysis activity of beet pulp.Fungus having high hydrolysis activity of beet pulp was screened from the soils collected from many parts of the nation. The bacterium hydrolyzed the beet pulp, and it produced arabinose related sugar. As a result of morphological observation and request analysis, it became clear that this strain was Aspergillus fumigatus.2.Electrical pre-treatment of beet pulp for its enzymatic hydrolysis.Beet pulp was pre-treated with 30 V of direct electric current and was hydrolyzed. The hydrolysis degree of beet pulp was increased by pre-treatment and it was found that the pre-treatment with direct electric current (1)causes swelling and atomization of beet pulp ; and (2)enhances the elution of hemicellulose from beet pulp, resulting in about the ability to extract 80% of the potential arabinose.3.Immobilization of fungi and its characteristic of beet pulp hydrolysis.The immobilization of Aspergillus fumigatus and/or Aspergillus niger having high hydrolysis activity of beet pulp was done by gas phase cultivation method using non-woven fabric made of rayon as a support material. The optimal temperature and the rate of hydrolysis was 40℃ and 70%, respectively.4.Production of usable materials from beet pulp.4-1 The extraction of usable materials from the beet pulp by electrical treatment.By the electrical treatment, about 28g of soluble materials were eluted from 100g of beet pulp. The components of them were arabinose(about 30%), xylose(about 9%), pectin(about 9%), galacturonic acids(about 3%) and others(49%).4-2 Production of antibacterial substance from beet pulp hydrolyzate.Beet pulp hydrolyzate was concentrated, and was fermented by Pediococcus acidilactici. The culture broth contained 14g/l of lactic acid and 9g/l of acetic acid, and had antibacterial activity against Erwiniw carotovora sub.sp.Carotovoa which was one of the plant pathogenic microbe.

1.甜菜渣高效水解真菌的筛选从全国各地采集的土壤中筛选出具有较高水解活性的真菌。该菌水解甜菜浆，产生与阿拉伯糖相关的糖。通过形态观察和要求分析，确定该菌株为烟曲霉（Aspergillus fumigatus）。2.电预处理甜菜浆进行酶解，甜菜浆经30 V直流电预处理后进行酶解。预处理提高了甜菜浆的水解度，发现直流电预处理（1）使甜菜浆溶胀、雾化;和（2）增强半纤维素从甜菜浆中的洗脱，从而能够提取80%的3.真菌的固定化及其水解甜菜浆的特性或Aspergillus尼日尔具有高的甜菜浆水解活性，通过气相培养方法，使用由人造丝制成的非织造织物作为支持材料进行。4.甜菜渣中有用物质的提取4 -1电处理甜菜渣中有用物质的提取通过电处理，从100 g甜菜渣中洗脱出约28 g可溶性物质。其主要成分为阿拉伯糖（约30%）、木糖（约9%）、果胶（约9%）、半乳糖醛酸（约3%）和其它（约49%）。4 -2甜菜浆水解液抗菌物质的生产甜菜浆水解液经浓缩后，用乳酸片球菌（Pediococcus acidilacidum）进行发酵。该发酵液含乳酸14 g/l，乙酸9 g/l，对植物病原菌胡萝卜软腐欧文氏菌（Erwiniw carotovora sub.sp.Carotovoa）有一定的抑菌作用。