Determination of the spatial arrangement of polypeptides in photosystem II by photo-and oxygen radical-induced protein cross-linking.
通过光和氧自由基诱导的蛋白质交联测定光系统 II 中多肽的空间排列。
基本信息
- 批准号:15570036
- 负责人:
- 金额:$ 2.05万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The initial purpose of the present research was to identify unknown polypeptides that are located closely to the reaction-center binding D1 protein in photosystem II by the use of chemical cross-linking and light-induced cross-linking methods. However, during the course of the study, we found that a metalloprotease FtsH is located near the core complex of photosystem II. We then have focused our efforts to clarify the roles of the FtsH protease in photosystem II. The following results were obtained.1.By chemical and photochemical cross-linking experiments, we found that the FtsH protease reacts with the D1 protein. These data as well as the membrane fractionation data strongly suggest that the FtsH protease can recognize the photodamaged D1 protein at the grana thylakoids.2.By moderate heat treatment (40゜C for 30 min) on spinach thylakoids, the D1 protein was cleaved. We found that the FtsH is responsible for the cleavage of the heat-damaged D1 protein.3.Using a cyanobacterial mutant lacking the FtsH protease, we confirmed that the the heat-or light-damaged D1 protein in the mutant cells is not removed, and thereby the growth and oxygen-evolving activity are significantly inhibited.All these results strongly suggest that the FtsH protease is essential for the quality control of photosystem II under light and heat stresses.
本研究的最初目的是通过化学交联和光诱导交联的方法鉴定位于光系统II中反应中心结合D1蛋白附近的未知多肽。然而,在研究过程中,我们发现,金属蛋白酶FtsH位于光系统II的核心复合物附近。然后,我们集中我们的努力,以澄清FtsH蛋白酶在光系统II中的作用。主要研究结果如下:1.通过化学交联和光化学交联实验,发现FtsH蛋白酶与D1蛋白发生了反应。这些数据以及膜分离数据都有力地表明FtsH蛋白酶可以识别光损伤的D1蛋白。2.适度热处理(40 ℃,30 min)菠菜类囊体,D1蛋白被切割。我们发现FtsH蛋白酶负责切割热损伤的D1蛋白。3.使用缺乏FtsH蛋白酶的蓝藻突变体,我们证实突变体细胞中的热或光损伤的D1蛋白没有被去除,因此,生长和氧气-所有这些结果都有力地表明,FtsH蛋白酶在光系统II的质量控制中是必不可少的。光和热应力。
项目成果
期刊论文数量(15)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Isolation and reactive characterization of oxygen-evolving photosystem II complexes retaining the PsbO, P and Q proteins from Euglena gracilis
细小眼虫中保留 PsbO、P 和 Q 蛋白的释氧光系统 II 复合物的分离和反应表征
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Jang;M.H.;Ha;K.;Joo;G.J.;Takamura;N.;S.Ohira;S.Ohira;高村典子;高村典子;T.Suzuki
- 通讯作者:T.Suzuki
Assay of photoinhibition of photosystem II and protease activity
光系统 II 的光抑制和蛋白酶活性的测定
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Makino;W.;Mikami;H.;Katano;N.;Nakagawa;M.;Takamura;N.;Y.Yamamoto;Y.Yamamoto
- 通讯作者:Y.Yamamoto
Quality control of photosystem II under light stress - Turnover of the aggregates of the D1 protein in vivo
光应激下光系统 II 的质量控制 - D1 蛋白体内聚集体的周转
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Tanaka;S;Ohsaki;N.;S.Ohira
- 通讯作者:S.Ohira
Pressure equilibrium and jump study on unfolding of 23-kDa protein from spinach photosystem II.
- DOI:10.1529/biophysj.104.050435
- 发表时间:2005-02
- 期刊:
- 影响因子:3.4
- 作者:Cui-yan Tan;Chun-he Xu;J. Wong;Jian-Ren Shen;Shinsuke Sakuma;Yasusi Yamamoto;R. Lange;C. Balny;K. Ruan
- 通讯作者:Cui-yan Tan;Chun-he Xu;J. Wong;Jian-Ren Shen;Shinsuke Sakuma;Yasusi Yamamoto;R. Lange;C. Balny;K. Ruan
Release and reactive-oxygen-mediated damage of the oxygen-evolving complex subunits of PSII during photoinhibition
- DOI:10.1093/pcp/pch027
- 发表时间:2004-02-01
- 期刊:
- 影响因子:4.9
- 作者:Henmi, T;Miyao, M;Yamamoto, Y
- 通讯作者:Yamamoto, Y
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
YAMAMOTO Yasusi其他文献
YAMAMOTO Yasusi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('YAMAMOTO Yasusi', 18)}}的其他基金
Aggregation of the reaction cenfer-binding protein of photosystem 11 under light stress and cell death
光系统11反应中心结合蛋白在光应激和细胞死亡下的聚集
- 批准号:
18570042 - 财政年份:2006
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Anovel degradation pathway of the D1 protein under light stress
光胁迫下D1蛋白的新降解途径
- 批准号:
13640650 - 财政年份:2001
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanism of the action of active oxygen species and proteases in the degradation of photosystem II complexes
活性氧和蛋白酶在光系统II复合物降解中的作用机制
- 批准号:
10640634 - 财政年份:1998
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Degration and removal of the proteins in photosystem II during photoinhibition
光抑制过程中光系统 II 中蛋白质的降解和去除
- 批准号:
08836007 - 财政年份:1996
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Formation of oxygen-evolving system of photosynthesis
光合作用释氧系统的形成
- 批准号:
02640523 - 财政年份:1990
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
Structural analysis of metal Inhibition Mechanisms in Photosystem II
光系统II中金属抑制机制的结构分析
- 批准号:
22K06100 - 财政年份:2022
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
RII Track-4:NSF: Tracking assembly intermediates of the Photosystem II oxygen-evolving complex
RII Track-4:NSF:跟踪光系统 II 放氧复合物的组装中间体
- 批准号:
2131863 - 财政年份:2022
- 资助金额:
$ 2.05万 - 项目类别:
Standard Grant
Regulation of light-harvesting systems in photosystem II supercomplexes
光系统 II 超级复合体中光捕获系统的调节
- 批准号:
21K15129 - 财政年份:2021
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Probing the structure and function of a super-rogue photosystem II complex involved in chlorophyll f synthesis
探讨参与叶绿素 f 合成的超级光系统 II 复合体的结构和功能
- 批准号:
BB/V002007/1 - 财政年份:2021
- 资助金额:
$ 2.05万 - 项目类别:
Research Grant
Photosystem II as a model protein for understanding metalloenzyme active site assembly
光系统 II 作为理解金属酶活性位点组装的模型蛋白
- 批准号:
10300881 - 财政年份:2021
- 资助金额:
$ 2.05万 - 项目类别:
Photosystem II as a model protein for understanding metalloenzyme active site assembly
光系统 II 作为理解金属酶活性位点组装的模型蛋白
- 批准号:
10473899 - 财政年份:2021
- 资助金额:
$ 2.05万 - 项目类别:
Structural and Electron Dynamics of the O-O bond Formation in Photosystem II
光系统 II 中 O-O 键形成的结构和电子动力学
- 批准号:
2004147 - 财政年份:2020
- 资助金额:
$ 2.05万 - 项目类别:
Standard Grant
Function of lipids in the dynamic assembly and recovery processes of photosystem II complex
脂质在光系统II复合体动态组装和恢复过程中的作用
- 批准号:
20K06701 - 财政年份:2020
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Structural analysis of the oxygen evolving center of photosystem II to gain insights into the water splitting reaction
光系统 II 的氧气放出中心的结构分析,以深入了解水分解反应
- 批准号:
20H03226 - 财政年份:2020
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Structural analysis of intermediate of Photosystem II using Cryo-EM
使用 Cryo-EM 对 Photosystem II 中间体进行结构分析
- 批准号:
19K22396 - 财政年份:2019
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)