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Studies on structural characterization and biological activities of diverse phosphogtycolipids in lower animals.

低等动物多种磷脂的结构特征和生物活性研究。

基本信息

批准号：
15570097
负责人：
SUGITA Mutsumi
金额：
$ 0.9万
依托单位：
Shiga University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

◎Structural analysis of oligosaccharide probes in glycosphingolipids containing inositolphosphate (phytoglycolipids and/or micoglycolipids)(1)Three novel series of glycosphingolipids containing inositolphosphate were isolated and purified from the marine annelid, P.ochelata. Their chemical structures completely characterized as Ins-P-Cer, Manα1-6Ins-P-Cer,Manα1-6(Manα1-4)Ins-P-Cer.(2)Mycoglycplipids were isolated from the seven edible fungi. For instance, these from Bunashimeji mushroom have been characterized as new extension structures of galactose and branched fucose residues with a common core structure (Manα1-2Ins-P-Cer).(3)Five novel zwitterionic glycosphingolipids have been isolated and purified from the mycelia of filamentous fungi, Acremonium sp. And also, a novel family of neutral glycosphingolipids was found in aureobasidin A-resistant Zygomycetes.◎Preparation of probes recognizing oligosaccharide and/or phosphate groups in phosphoglycolipid moleculesAntisera against Manα1-2Ins-P-Cer and Manα1-2(Fucα1-5)Ins-P-Cer isolated from the marine annelida (lugworm), T.heterochetus have been elicited in rabbits by repeated injection of a mixture of hapten-methylated bovine seraum albumin with Freund's adjuvant.◎Structural elucidation of sphingolipids from pearl oyster, P.martensiiSphingolipids, ceramide aminoethylphosphonate, sphingomyelin and cerebrosides were isolated and their chemical structures were characterized. The ceramide moieties of these lipids consisted of palmitic, margaric and stearic acids and their 2-hydroxy acids as fatty acids, and sphingosine, Δ^<4,8>-sphingadienine and 9-methyl-Δ^<4,8,10>-sphingatrienine as sphingoids.

<$含肌醇磷酸鞘糖脂（植物糖脂和/或微糖脂）中寡糖探针的结构分析（1）从海洋环节动物P. ochelata中分离纯化了三个新的含肌醇磷酸鞘糖脂系列。它们的化学结构完全确定为Ins-P-Cer、Manα1- 6 Ins-P-Cer、Manα1-6（Manα1-4）Ins-P-Cer。（2）从7种食用菌中分离出菌糖脂。例如，来自Bunashimeji蘑菇的这些已被表征为具有共同核心结构的半乳糖和支链岩藻糖残基的新延伸结构（Manα1- 2 Ins-P-Cer）。（3）从丝状真菌Acremonium sp.的菌丝体中分离纯化了5种新型两性离子鞘糖脂，从海洋环节动物（沙蚕）中分离的Manα1- 2（Fucα1-5）Ins-P-Cer和Manα1-2（Fucα1-5）Ins-P-Cer抗血清，用半抗原-甲基化牛血清白蛋白与弗氏佐剂的混合物反复注射家兔体内，诱发异刺毛癣菌（T.heterochetus）。这些脂质的神经酰胺部分由作为脂肪酸的棕榈酸、十七烷酸和硬脂酸及其2-羟基酸和作为鞘氨醇的鞘氨醇、Δ^<4，8>-鞘二烯碱和9-甲基-Δ^<4，8，10>-鞘三烯碱组成。