Roles of WAVE in transformation of cell signals to filopodia protrusion at neuronal growth cone

WAVE 在细胞信号转化为神经元生长锥丝状伪足突起中的作用

• 批准号: 15570136
• 负责人: MIYAMOTO Shigeaki
• 金额: $ 2.43万
• 依托单位: Kyushu Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570136/
• 关键词: growth cone; lamellipodia; filopodia; WAVE; actin; fascin; Lasp-1; Lasp-2; lasp-1; lasp-2; NG108; EGFP; leucine-zipper-like motif

Neuronal cells extend the growth cones forming lamellipodia and filopodia in the response to the external guidance factors. The reorganization of actin cytoskeleton supply the mechanical force in these process formations, and actin bundle grown in this process form a core of filopodium. We have studied the roles of WAVEs that transfer cell signals from small G-protein, Rho, to the rearrangements of actin network. By expressing WAVE isoforms tagged with fluorescent proteins in NG108 we obtained the following results.(1)WAVE1 localized only at the leading edge of lamellipodia, and WAVE2 and WAVE3 were localized also at the tips of filopodia. Especially WAVE2 were concentrated.(2)We identified the leucine-zipper like motif between 52th and 82th of WAVE2 at amino terminal controlled the localization at filopodium tip.(3)The factors that bundle actin filaments are necessary to elongate the filopodia. We show that fascin takes a role to bundle actin filaments in filopodia.(4)A novel protein family, Lasp-family(Lasp-1 and Lasp-2), bundle the actin filaments in filopodia similarly to fascin.(5)Lasp-1,Lasp-2 and fascin interact with actin bundle by non-covalent bond, independently of retrograde flow of actins.

神经元细胞在对外部引导因素的反应中扩展了形成层状脂蛋白的生长锥和丝状细胞。肌动蛋白细胞骨架的重组在这些过程形成中提供机械力，而在此过程中生长的肌动蛋白束构成了丝脂的核心。我们研究了将细胞信号从小的G蛋白Rho传递到肌动蛋白网络的重排的波的作用。通过在NG108中表达用荧光蛋白标记的波浪同工型，我们获得了以下结果。（1）仅位于板层的前缘的WAVE1，Wave2和Wave3也位于丝状尖端。 （2）我们识别出亮氨酸的基序，如氨基末端的第52至82位波2的序列，控制了在丝状尖端处的定位。 We show that fascin takes a role to bundle actin filaments in filopodia.(4)A novel protein family, Lasp-family(Lasp-1 and Lasp-2), bundle the actin filaments in filopodia similarly to fascin.(5)Lasp-1,Lasp-2 and fascin interact with actin bundle by non-covalent bond, independently of retrograde flow of actins.

项目成果

Cytoskeleton and Cell Motility

• DOI: 10.1007/978-0-387-30440-3_112
• 发表时间: 2011-05
• 作者: T. Risler

IRSP53 is co-localized with WAVE2 at the tips of protruding lamellipodla and filopodia independently of MENA

IRSP53 与 WAVE2 共同定位在突出的板状足和丝状伪足的尖端，独立于 MENA

• 发表时间: 2003
• 期刊: Journal of Cell Science 116
• 作者: Saitoh;O.;Odagiri;M.;Nakagawa Hiroyuki
• 通讯作者: Nakagawa Hiroyuki

ANovel LIM and SH3 protein highly expressing in chicken brain

鸡脑中高表达的新型 LIM 和 SH3 蛋白

• 发表时间: 2004
• 期刊: Blochem.Biophys.Res.Com. 313
• 作者: Terasaki Asako

The pre-mRNA-splicing factor SFa66 functions as a microtubule bindihg and-bundling protein

前 mRNA 剪接因子 SFa66 作为微管结合蛋白发挥作用

• 发表时间: 2004
• 期刊: Biochem.J. 15
• 作者: Takenaka;K.
• 通讯作者: K.

IRSp53 is co-localized with WAVE2 at the tips of protruding lamellipodia and filopodia independently of Mena.

IRSp53 与 WAVE2 共同定位在突出的板状伪足和丝状伪足的尖端，独立于 Mena。

• 发表时间: 2003
• 期刊: Journal of Cell Science Vol.116
• 作者: Hiroyuki;Nakagawa;et al.
• 通讯作者: et al.