Phospholipid-nanotube containing a peptide, Hel 13-5, as a model of transport vesicles in cell.

含有肽 Hel 13-5 的磷脂纳米管，作为细胞内运输囊泡的模型。

• 批准号: 15570141
• 负责人: LEE Sannamu
• 金额: $ 2.43万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570141/
• 关键词: Nanotube; Amphuphilic peptide; Gold nanowire; Phospholipid; Lipisome; Neutron scattering in solution; Lipid helix; Structural biolog; 両親媒性ベプチド; 細胞内小胞輸送; ペプチド; 両親媒性モデルペプチド

Cellular organelles, such as the Golgi apparatus and the endoplasmic reticulum, adopt characteristic structures depending on their function. While the tubular shapes of these structures result from complex protein-lipid interactions that are not fully understood, some fundamental machinery must be required. We show here that a de novo-designed 18-mer amphipathic a-helical peptide, Hel 13-5, transforms spherical liposomes made from a Golgi-specific phospholipid mixture into nanotubules on the scale of, and resembling the shape of, the nanotubules that form the Golgi apparatus. Furthermore, we show that that the size and the shape of such nanotubules depend on lipid composition, and peptide properties such as length, and the ratio of hydrophobic to hydrophilic amino acids. Although the question of precisely how nature engineers organellar membranes remains unknown, our simple system provides a basic set of tools to begin addressing this question.

细胞器，如高尔基体和内质网，根据其功能采用特征结构。虽然这些结构的管状形状是由复杂的蛋白质-脂质相互作用引起的，但尚未完全了解，必须需要一些基本的机械。我们在这里表明，一个新的设计的18聚体两亲性α-螺旋肽，Hel 13-5，从高尔基体特异性磷脂混合物制成的球形脂质体转化成纳米管的规模，并类似的形状，形成高尔基体的纳米管。此外，我们表明，这种纳米管的大小和形状取决于脂质组成，和肽的性质，如长度，和疏水性的亲水性氨基酸的比例。虽然大自然如何精确地设计细胞器膜的问题仍然未知，但我们的简单系统提供了一套基本的工具来开始解决这个问题。

项目成果

A monolayer study on three binary mixed systems of dipalmitol phosphatidylcholine with choresterol, cholestanol and stigmasterol

二棕榈醇磷脂酰胆碱与菜甾醇、胆甾醇、豆甾醇三种二元混合体系的单层研究

• 发表时间: 2004
• 期刊: Colloids Surface B, Biointerfaces 33
• 作者: M.Kodama;O.Shibata;S.Nakamura;S.Lee;G.Sugihara
• 通讯作者: G.Sugihara

Nanotubules formed by highly hydrophobic amphiphilic alpha-helical peptides and natural phospholipids

由高度疏水性两亲性α螺旋肽和天然磷脂形成的纳米管

• 发表时间: 2003
• 期刊: Biophysical Journal 84
• 作者: Furuya;T.;Kiyota;T.;Lee;S.
• 通讯作者: S.

The effect of cholesterol and monosialoganglioside (GMI) on the release and aggregation of Amyloid beta-peptide from liposomes prepared from brain membrane-like lipids

胆固醇和单唾液酸神经节苷脂（GMI）对脑膜样脂质脂质体中β-淀粉样蛋白肽的释放和聚集的影响

• 发表时间: 2004
• 期刊: J. Biol. Chem. 279
• 作者: Y.Tashima;R.Oe;S.Lee;G.Sugihara;E.J.Chambers;M.Takahashi;T.Yamada
• 通讯作者: T.Yamada

Tashima, Y., Oe, R., Lee, S., その他: "The effect of cholesterol and monosialoganglioside (GM1) on the release and aggregationoid beta-peptide from liposomes prepared from brain membrane-like lipids"J.Biol.Chem.. 279(In press). (2004)

Tashima, Y., Oe, R., Lee, S., et al.：“胆固醇和单唾液酸神经节苷脂 (GM1) 对从脑膜样脂质制备的脂质体中释放和聚集的 β-肽的影响”J.Biol .化学.. 279（印刷中）（2004）。

Kodama, M., Shibata, O., Nakamura, S., Lee, S., Sugihara, G.: "A monolayer study on three binary mixed systems of dipalmitol phosphatidyl choline with choresterol cholestanol and stiamasterol"Colloids Surf. B, Biointerfaces. 33. 211-226 (2004)

Kodama, M.、Shibata, O.、Nakamura, S.、Lee, S.、Sugihara, G.：“二棕榈醇磷脂酰胆碱与鸡甾醇胆甾醇和豆甾醇的三个二元混合系统的单层研究”胶体冲浪。