Effect of membrane dynamics on affecting molecular behavior of trans-membrane a -helical peptides

膜动力学对跨膜α-螺旋肽分子行为的影响

基本信息

  • 批准号:
    12680665
  • 负责人:
  • 金额:
    $ 2.3万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2001
  • 项目状态:
    已结题

项目摘要

The roles of peptide-peptide charged interaction and lipid phase separation in helix-helix association in lipid bilayers were investigated using a model peptide, P24, as a transmembrane a-helical peptide, and its four analogues. Fluorescence ammo acids, tryptophan (P24W) and pyrenylalanine (P24Pya), were introduced into the sequence of P24, respectively. Association of these peptides permits the resonance excitation energy transfer between tryptophan in P24W and pyrenylalanine in P24Pya or excimer formation between P24Pya themselves. To evaluate the effect of charged interaction on the association between a-helical transmembrane segments A in membrane proteins, charged amino acids, glutamic acid (P24EW) and lysine (P24Kpya), were introduced into P24W and P24Pya, respectively. Energy transfer experiments indicated that the charged interaction between the positive charge of lysine residue in P24KPya and the negative charge of glutamic acid residue in P24EW did not affect the aggregation of transmembrane peptides in lipid membranes. As the content ratio of sphingomyelm and cholesterol was increased in the egg PC, the stronger excimer fluorescence spectra of P24Pya were observed, indicating that the co-existence of SM and Ch in PC liposomes, that is, the raft of SM and Ch, promotes the aggregation of thef a-helical transmembrane peptides in lipid bilayers. Since the increase in the contents of SM and Ch leads to the decrease in the content of liquid crystalline order phase, the moving area of transmembrane peptides might be limited in the liposomes, resulting in easy formation of the excimer in the presence of the lipid-raft
以跨膜α-螺旋肽P24及其4个类似物为模型肽,研究了肽-肽电荷相互作用和脂相分离在脂双层螺旋-螺旋缔合中的作用。将荧光氨基酸色氨酸(P24 W)和芘基丙氨酸(P24 Pya)分别引入P24的序列中。这些肽的缔合允许P24 W中的色氨酸和P24 Pya中的芘基丙氨酸之间的共振激发能量转移或P24 Pya自身之间的激基缔合物形成。为了评价带电相互作用对膜蛋白中α-螺旋跨膜片段A之间缔合的影响,将带电氨基酸谷氨酸(P24 EW)和赖氨酸(P24 Kpya)分别引入P24 W和P24 Pya中。能量传递实验表明,P24 KPya中赖氨酸残基的正电荷与P24 EW中谷氨酸残基的负电荷之间的相互作用不影响跨膜肽在脂膜上的聚集。随着卵磷脂中鞘磷脂和胆固醇含量比例的增加,P24 Pya的激基缔合物荧光光谱增强,说明卵磷脂脂质体中SM和Ch的共存,即SM和Ch的筏,促进了α-螺旋跨膜肽在脂质双层中的聚集。由于SM和Ch含量的增加导致液晶有序相含量的减少,因此跨膜肽在脂质体中的移动区域可能受到限制,从而导致在脂筏存在下容易形成激基缔合物

项目成果

期刊论文数量(18)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
E.Matsumoto, T.Kiyota, S.Lee, G.Sugihara, その他5名: "Study on the packing geometry, stoichiometry and membrane-interaction of three analogs related to a pore-forming small globular protein (SGP)"Biopolymers. 56. 96-108 (2001)
E.Matsumoto、T.Kiyota、S.Lee、G.Sugihara 和其他 5 人:“与成孔小球状蛋白 (SGP) 相关的三种类似物的堆积几何形状、化学计量和膜相互作用的研究”生物聚合物。 56. 96-108 (2001)
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    0
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Matsumoto E., Kiyota T., Lee S., Sugihara G., Yashita S., Meno H., Aso Y., Sakamoto H., Miellerby H.: "Study on the packing geometry, stoichiometry and membrane-interaction of three analogs related to a pore-forming small globular protein (SGP)"Biopolymer
Matsumoto E.、Kiyota T.、Lee S.、Sugihara G.、Yashita S.、Meno H.、Aso Y.、Sakamoto H.、Miellerby H.:“关于三种材料的堆积几何形状、化学计量和膜相互作用的研究
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Matsutani M., Wako H., Sakamoto H., Lee S., Sugihara G.: "Effect of hydrophobic core amino acid residues of p53 oligomerization domain on the stability of three dimensional structure"Peptide Science, 2000, ed. T. Shioiri, Japanese Peptide Soc. Osaka. 285-
Matsutani M.、Wako H.、Sakamoto H.、Lee S.、Sugihara G.:“p53 寡聚化结构域的疏水性核心氨基酸残基对三维结构稳定性的影响”肽科学,2000 年,编辑。
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    0
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K.Shindo, K.Shinozaki K.Kami, K.Anzai, S.Lee, その他3名: "Solution Structure of Micelle-bound H5 Peptide (427-452) : A Pri-mary Structure Corresponding to the Pore-Forming Region of the Voltage Dependent Potassium Channel"Biochimica Biophysica Acta. 1545. 153
K.Shindo、K.Sinozaki K.Kami、K.Anzai、S.Lee 和其他 3 人:“胶束结合 H5 肽 (427-452) 的溶液结构:对应于孔形成区域的基本结构电压依赖性钾通道的研究“生物化学生物物理学法. 1545. 153
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    0
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Lee S, Furuya T, Kiyota T, Takami N, Murata K, Niidome Y, Bredesen DE, Ellerby HM, Sugihara G.: "De novo designed peptide transforms Golgi-specific lipids into nanotubules resembling those of the Golgi apparatus"J. Biol. Chem. 267. 41224 (2001)
Lee S、Furuya T、Kiyota T、Takami N、Murata K、Niidome Y、Bredesen DE、Ellerby HM、Sugihara G.:“从头设计的肽将高尔基体特异性脂质转化为类似于高尔基体的纳米管”J.
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LEE Sannamu其他文献

LEE Sannamu的其他文献

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{{ truncateString('LEE Sannamu', 18)}}的其他基金

Phospholipid-nanotube containing a peptide, Hel 13-5, as a model of transport vesicles in cell.
含有肽 Hel 13-5 的磷脂纳米管,作为细胞内运输囊泡的模型。
  • 批准号:
    15570141
  • 财政年份:
    2003
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The evolution of oligomerization factors of membrane-spanning alpha-helical segments into lipid bilayers
跨膜α螺旋片段寡聚因子向脂质双层的演化
  • 批准号:
    09680661
  • 财政年份:
    1997
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
De novo synthesis of a small globular protein SGP and its insertion into lipid bilay
小球状蛋白SGP的从头合成及其插入脂质双层
  • 批准号:
    07680729
  • 财政年份:
    1995
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Synthesis of Transmembrane Segment of A Single Spanning Protein, Isk, Forming Potassium Channel and Its Interaction with Phospholipid Bilayr
形成钾通道的单跨膜蛋白 Isk 跨膜片段的合成及其与磷脂双层的相互作用
  • 批准号:
    05680584
  • 财政年份:
    1993
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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