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Study of the novel Nodal target genes : molecular mechanism of inhibition of the FGF signaling and involvement of mesoderm formation during Xenopus development.

新型 Nodal 靶基因的研究：在非洲爪蟾发育过程中抑制 FGF 信号传导和参与中胚层形成的分子机制。

基本信息

批准号：
15570172
负责人：
WATANABE Minoru
金额：
$ 2.05万
依托单位：
University of Tokushima (2004)Gifu University (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570172/
关键词：
Xenopus transcription factor morphogenesis Nodal FGF mesoderm transcriptional regulation signal transduction

项目摘要

The XSPR2b gene was isolated as the genes induced by FAST-1, a major transcriptional regulator of the Nodal signaling, from Xenopus laevis. It was shown that the expression of the XSPR2b gene was induced not only by the Nodal/FAST-1 signaling, but also the FGF signaling by using animal cap assay. This gene encodes transcription factor and is expressed mainly in the mesodermal region during the early Xenopus development. The expression domain is overlapped with those of Xbra gene, a major gene of mesoderm formation. Overexpression of the XSPR2b gene induced the ectopic expression of the Xbra gene in the embryos, suggesting the functional interaction between these genes. In the animal cap cells, overexpression of the Xbra gene itself could induce some mesodermal genes, whereas XSPR2b gene could not. When both genes were co-expressed in the cap cells, induction of the Xbra-target genes were up regulated. However, the expression of the none-Xbra target genes was not induced. These results suggested that XSPR2b gene has a role in mesoderm formation of Xenopus, through Xbra gene. By the reporter gene assay, wild type XSPR2b gene was shown to encode transcriptional activator. The molecular mechanism of the XSPR2b gene to induce the Xbra gene is currently under investigation.

XSPR2b 基因是从非洲爪蟾中分离出来的，是由 FAST-1 诱导的基因，FAST-1 是 Nodal 信号传导的主要转录调节因子。通过动物帽实验表明，XSPR2b 基因的表达不仅受到 Nodal/FAST-1 信号传导的诱导，而且还受到 FGF 信号传导的诱导。该基因编码转录因子，主要在非洲爪蟾发育早期的中胚层区域表达。该表达域与中胚层形成的主要基因Xbra基因的表达域重叠。 XSPR2b 基因的过度表达诱导胚胎中 Xbra 基因的异位表达，表明这些基因之间存在功能相互作用。在动物帽细胞中，Xbra基因本身的过表达可以诱导一些中胚层基因，而XSPR2b基因则不能。当两种基因在帽细胞中共表达时，Xbra 靶基因的诱导上调。然而，非 Xbra 靶基因的表达并未被诱导。这些结果表明XSPR2b基因通过Xbra基因在爪蟾中胚层形成中发挥作用。通过报告基因检测，野生型XSPR2b基因显示编码转录激活因子。 XSPR2b基因诱导Xbra基因的分子机制目前正在研究中。