Molecular analysis of muscarinic receptors and regulation mechanism of dilation in the rat iris.

大鼠虹膜毒蕈碱受体的分子分析及扩张调节机制。

基本信息

  • 批准号:
    08672525
  • 负责人:
  • 金额:
    $ 1.6万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1997
  • 项目状态:
    已结题

项目摘要

The amount of mRNAs of muscarinic acetylcholine receptor subtypes, m1, m2, m3, m4 and m5, in the rat iris was determined quasi-quantitatively using RT-PCR methods. The mRNA expression levels of m2 and m3 were higher than those of m1 and m5 in the rat iris as in other smooth muscle tissues. It was characteristic in the rat iris that the m4 mRNA expression level was high. In addition, the cloning of cDNAs encoding m2, m3 and m4 subtypes in the rat iris was performed using RT-PCR methods. Amino acids sequence of m2 subtype in the rat iris included nine differences in comparison with that in the heart, whereas it was 100% identical to genomic one. To examine the mechanisms underlying the parasympathectomy-induced effects in the rat iris smooth muscles, the mRNA expression levels of muscarinic receptor subtypes were determined in iris from ciliaryganglionectomized rats. The results suggested that the parasympathectomy does not markedly affect the mRNA levels. It was also clarified that the intracellular Ca^<2+> concentration ([Ca^<2+>]_i) in the rat iris dilator under the resting conditions is higher than those in the iris sphincter and many other smooth muscles which do not have an inherent tone. Since the muscarinic receptor stimulation resulted in the decrease in [Ca^<2+>]_i in the rat iris dilator, the muscarinic relaxation appeared to be due to the decrease in [Ca^<2+>]_i. In denervated iris dilators, muscarinic receptor stimulation induced neither the decrease in [Ca^<2+>]_i nor the relaxation.
采用RT-PCR方法准定量测定大鼠虹膜中毒蕈碱乙酰胆碱受体m1、m2、m3、m4和m5亚型mrna的数量。与其他平滑肌组织一样,大鼠虹膜中m2和m3的mRNA表达量高于m1和m5。大鼠虹膜的特点是m4 mRNA表达量高。此外,采用RT-PCR方法克隆大鼠虹膜中m2、m3和m4亚型的编码cdna。大鼠虹膜m2亚型氨基酸序列与心脏m2亚型氨基酸序列有9个差异,但与基因组序列完全相同。为了研究副交感神经切除对大鼠虹膜平滑肌影响的机制,我们测定了去睫状神经节大鼠虹膜中毒蕈碱受体亚型mRNA表达水平。结果表明,副交感神经切除对mRNA水平无明显影响。研究还表明,静息状态下大鼠虹膜扩张肌细胞内Ca^<2+>浓度([Ca^<2+>]_i)高于虹膜括约肌和许多其他没有固有张力的平滑肌。由于毒蕈碱受体刺激导致大鼠虹膜扩张器[Ca^<2+>]_i降低,毒蕈碱松弛似乎是由于[Ca^<2+>]_i降低所致。在去神经虹膜扩张器中,毒蕈碱受体刺激既没有引起[Ca^<2+>]_i的降低,也没有引起松弛。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Namie Hara, Yuji Imaizumi and Minoru Watanabe: "Measurement of intracellular calcium concentration in iris smooth muscle of the rat" Japanese Journal of Pharmacology. 73, Supplement 1. O-28 (1997)
Namie Hara、Yuji Imaizumi 和 Minoru Watanabe:“大鼠虹膜平滑肌细胞内钙浓度的测量”日本药理学杂志。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

WATANABE Minoru其他文献

WATANABE Minoru的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('WATANABE Minoru', 18)}}的其他基金

Development of a 3-dimensional optoelectronic mechanical programmable device and its dynamic circuit implementation
3维光电机械可编程器件的研制及其动态电路实现
  • 批准号:
    24300017
  • 财政年份:
    2012
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
A real-time image recognition system usinga holographic memory and a microelectromechanical system (MEMS)
使用全息存储器和微机电系统(MEMS)的实时图像识别系统
  • 批准号:
    23650087
  • 财政年份:
    2011
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
High-speed optically reconfigurable gate array exploiting a MEMS and a laser array
利用 MEMS 和激光阵列的高速光学可重构门阵列
  • 批准号:
    20200027
  • 财政年份:
    2008
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
Programmable optically reconfigurable gate array and its writer
可编程光可重构门阵列及其写入器
  • 批准号:
    20560322
  • 财政年份:
    2008
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Study of the novel Nodal target genes : molecular mechanism of inhibition of the FGF signaling and involvement of mesoderm formation during Xenopus development.
新型 Nodal 靶基因的研究:在非洲爪蟾发育过程中抑制 FGF 信号传导和参与中胚层形成的分子机制。
  • 批准号:
    15570172
  • 财政年份:
    2003
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The mechanisms underlying nonspecific supersensitivity induced by parasympathetic denervation in rat iris sphincter
大鼠虹膜括约肌副交感神经去神经诱导非特异性超敏反应的机制
  • 批准号:
    10672050
  • 财政年份:
    1998
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular Diversity of K Channels in Cardiovascular System.
心血管系统中 K 通道的分子多样性。
  • 批准号:
    08044313
  • 财政年份:
    1996
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Molecular piolcgical analysises of muscarinic receptore in the rat iris dilator
大鼠虹膜扩张器毒蕈碱受体的分子生物学分析
  • 批准号:
    06672195
  • 财政年份:
    1994
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Molecular biology of vascular and cordiac k channels
血管和心脏 k 通道的分子生物学
  • 批准号:
    06044192
  • 财政年份:
    1994
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Pharmacology of peconstitued synapses in co-culture of autonomic neurons and smooth mescle cells
自主神经元和平滑肌细胞共培养中特构突触的药理学
  • 批准号:
    04671363
  • 财政年份:
    1992
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了