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Molecular mechanism of the differentiation and tissue formation from the common primordium of adrenal cortex and gonads.

肾上腺皮质和性腺共同原基分化和组织形成的分子机制。

基本信息

批准号：
15570180
负责人：
HATANO Osamu
金额：
$ 2.24万
依托单位：
Nara Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570180/
关键词：
adrenal cortex gonads steroid hormone differentiation development adrenal cortex gonads development Pref-1 WT1 Ad4BP SF-1 Emx2 differentiation 発生分化 IZA

项目摘要

The expression profiles of Ad4BP/SF-1 in rat embryos demonstrated that steroid-hormone-producing cells of adrenal cortex and gonads are derived from a common precursor named the "adreno-genital primordium". To address the question of the molecular mechanism that underlies the differentiation of the adrenal cortex and gonads, we raised new antibodies against some of the differentiation related factors (Pref-1/ZOG, WT1, Ad4BP/SF-1, Emx2), and examined the expression of these factors and the relationship to other factors. Pref-1 was expressed in the adreno-genital primordium slightly wider than Ad4BP/SF1. The expression of Pref-1 decreased during the differentiation of adrenal cortex and gonads, and Pref-1 was supposed to have function to keep cells in undifferentiated state. In the adult adrenal cortex, the expression of Pref-1 is restricted to the zona glomerulosa, whereas the expression of adrenal inner zone antigen (IZA) was restricted to the zona fasciculata and zona reticularis. The expression profiles of Pref-1 and IZA was complementary. IZA was found to bound a haem chromophore and was identical with membrane-associated progesterone receptor (MPR). IZA enhanced progesterone 21-hydroxylase reaction. Pref-1 was also found to be abundantly expressed in pituitary. Emx2 is essential for the formation of gonads. Emx2 was also expressed in the marginal zone of cerebral cortex and essential for the survival of reelin-positive cells, whereas Pax6 was also involved in the specification of reelin-positive cells in the cortical marginal zone, potentially in a manner opposite to the positive role of Emx2.

Ad 4 BP/SF-1在大鼠胚胎中的表达谱表明，肾上腺皮质和性腺的类固醇激素产生细胞来自一个共同的前体，称为“肾上腺生殖原基”。为了解决肾上腺皮质和性腺分化的分子机制的问题，我们提出了一些分化相关因子（Pref-1/ZOG，WT 1，Ad 4 BP/SF-1，Emx 2）的新抗体，并检查这些因子的表达和与其他因子的关系。Pref-1在肾上腺生殖原基中的表达比Ad 4 BP/SF 1稍宽。在肾上腺皮质和性腺分化过程中，Pref-1的表达逐渐减少，推测Pref-1可能具有维持细胞未分化状态的功能。在成人肾上腺皮质中，Pref-1的表达仅限于肾小球，而肾上腺内带抗原（IZA）的表达仅限于束状核和网状核。Pref-1和IZA的表达谱具有互补性。发现IZA结合血红素发色团，并且与膜相关孕酮受体（MPR）相同。IZA促进孕酮21-羟化酶反应。Pref-1在垂体中也有大量表达。Emx 2对性腺的形成至关重要。Emx 2也在大脑皮质边缘区表达，对于卷轴蛋白阳性细胞的生存至关重要，而Pax 6也参与皮质边缘区卷轴蛋白阳性细胞的特化，其方式可能与Emx 2的积极作用相反。