Reproductive sturucture of anguilloid fishes as revealed by genome typing for their

2003- 2004年期间，在九州东部的日向田近海沃茨共进行了171次净取样。这些取样器是用来收集鳗卵的。采用四层拖曳法（0、5、15、30 m深度），用直径为1.6 m、网眼为0.5 mm的环网进行采样。采样工作在夏季最为密集，然而，在一年中，每月的样本。共采集了19种1,215枚卵。根据卵径、油球、色素沉着、肌节数等形态特征，可将其划分为Muraenidae、Ophichthidae、Congridae、Muraenesostomatiae、Nettastomatidae等科。为了确定物种水平的鉴定，将这些卵的线粒体DNA（16 SrRNA）序列与成虫的进行比较。从19种卵中鉴定出25种。大多数物种在夏季表现出高度季节性的丰富模式。结果表明：海鳗科无油珠或油珠少，直径2.30 -5.50 mm;蛇鳗科无油珠或油珠多，直径1.62-4.40 mm;网口鱼科无油珠或油珠多，直径1.98-3.24 mm;海鳗科油珠多，直径2.00 -2.46 mm。

During 2003-04,a total of 171 net sampling were made waters off Hyuganada, east Kyushu. These sampling ware designed to collect anguilloid eggs. Samplings were made with 1.6 m diameter ring net constructed with 0.5 mm mesh using four layers towing methods (0,5,15,30m depth). Sampling effort was most intensive during summer, however, monthly samples were taken around year. A total 1,215 eggs comprising 19 species were taken. Families represented include the Muraenidae, Ophichthidae, Congridae, Muraenesocidae, and Nettastomatidae from the morphological characteristics, for example, egg diameter, oil globule, pigmentation and number of myomeres of newly hatched larvae. To determine species level identification, mitochondrial DNA(16SrRNA) sequences of those eggs were compared those of adults. Twenty five species were recoginaized from those 19 types eggs. Most species showed highly seasonal patterns of abundance during summer. Based on the present study, following major characters of eggs are revealed ; Muraenidae : absent or few oil globules,2.30-5.50 mm diameter ; Ophichthidae : absent or many oil globules, 1.62-4.40 mm diameter ; Nettastomatidae : absent or two oil globules, 1.98-3.24 mm diameter, Muraenesocidae : many oil gbobules,2.00-2.46 mm diameter.