Reproductive sturucture of anguilloid fishes as revealed by genome typing for their

通过基因组分型揭示鳗鱼的生殖结构

• 批准号: 15580165
• 负责人: MOCHIOKA Noritaka
• 金额: $ 2.43万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580165/
• 关键词: Anguilliformes; mitochondrial DNA; 16SrRNA; Egg; Muraenesocidae; Ophichthidae; Nettastomatidae; Muraenidae; Ohichthidae; ウナギ目魚類

During 2003-04,a total of 171 net sampling were made waters off Hyuganada, east Kyushu. These sampling ware designed to collect anguilloid eggs. Samplings were made with 1.6 m diameter ring net constructed with 0.5 mm mesh using four layers towing methods (0,5,15,30m depth). Sampling effort was most intensive during summer, however, monthly samples were taken around year. A total 1,215 eggs comprising 19 species were taken. Families represented include the Muraenidae, Ophichthidae, Congridae, Muraenesocidae, and Nettastomatidae from the morphological characteristics, for example, egg diameter, oil globule, pigmentation and number of myomeres of newly hatched larvae. To determine species level identification, mitochondrial DNA(16SrRNA) sequences of those eggs were compared those of adults. Twenty five species were recoginaized from those 19 types eggs. Most species showed highly seasonal patterns of abundance during summer. Based on the present study, following major characters of eggs are revealed ; Muraenidae : absent or few oil globules,2.30-5.50 mm diameter ; Ophichthidae : absent or many oil globules, 1.62-4.40 mm diameter ; Nettastomatidae : absent or two oil globules, 1.98-3.24 mm diameter, Muraenesocidae : many oil gbobules,2.00-2.46 mm diameter.

在2003 - 04年期间，在东京胡苏（East Kyushu）的Hyuganada附近总共有171个净采样。这些采样旨在收集Anguilloid卵。使用四层牵引方法（0,5,15,15,30m的深度）用直径1.6 m直径的环网进行采样。在夏季，采样工作最密集，但是，每月样品大约在一年左右进行。总共取了1,215个卵，其中包括19种。代表的家庭包括来自形态学特征的Muraenidae，Ophichthidae，控齿科，Muraesocidae和Nettastomatidae，例如，卵直径，油球，色素沉着和新近孵化的幼虫的肌瘤数量。为了确定物种水平的鉴定，比较了这些卵的线粒体DNA（16sRRNA）序列。从这19种鸡蛋中重生了25种。大多数物种在夏季表现出高度季节性的丰度。基于本研究，揭示了以下卵的主要特征。 Muraenidae：缺乏或少数油球，直径为2.30-5.50毫米； Ophichthidae：缺乏或许多油球，直径为1.62-4.40毫米； NettastomatIdae：缺少或两个油球，直径1.98-3.24毫米，Muraenesocidae：许多油gbobules，直径2.00-2.46毫米。