Reproductive sturucture of anguilloid fishes as revealed by genome typing for their

2003- 2004年期间，在东九州Hyuganada海域共进行了171次净抽样。这些取样器是用来收集鳗类动物的卵的。采用直径1.6 m、网孔0.5 mm的环网，采用4层拖曳法（深度0、5、15、30m）进行取样。采样工作在夏季最为密集，但在一年左右每月采样一次。共取卵1,215枚，包括19个品种。从卵径、油球、色素沉着和新孵化幼虫的肌粒数量等形态特征来看，代表的科包括村蝇科、蛇蝇科、孔雀科、村蝇科和网蝇科。为了确定物种水平的鉴定，将这些卵的线粒体DNA（16SrRNA）序列与成年卵进行了比较。从这19种卵中鉴定出25种。大多数物种在夏季表现出高度的季节性丰度。根据目前的研究，揭示了鸡蛋的以下主要特征；村蝇科：无或很少油球，直径2.30-5.50毫米；蛇蛾科：无或许多油球，直径1.62-4.40毫米；网蝽科：无或两个油球，直径1.98-3.24毫米；蠓科：许多油球，直径2.00-2.46毫米。

During 2003-04,a total of 171 net sampling were made waters off Hyuganada, east Kyushu. These sampling ware designed to collect anguilloid eggs. Samplings were made with 1.6 m diameter ring net constructed with 0.5 mm mesh using four layers towing methods (0,5,15,30m depth). Sampling effort was most intensive during summer, however, monthly samples were taken around year. A total 1,215 eggs comprising 19 species were taken. Families represented include the Muraenidae, Ophichthidae, Congridae, Muraenesocidae, and Nettastomatidae from the morphological characteristics, for example, egg diameter, oil globule, pigmentation and number of myomeres of newly hatched larvae. To determine species level identification, mitochondrial DNA(16SrRNA) sequences of those eggs were compared those of adults. Twenty five species were recoginaized from those 19 types eggs. Most species showed highly seasonal patterns of abundance during summer. Based on the present study, following major characters of eggs are revealed ; Muraenidae : absent or few oil globules,2.30-5.50 mm diameter ; Ophichthidae : absent or many oil globules, 1.62-4.40 mm diameter ; Nettastomatidae : absent or two oil globules, 1.98-3.24 mm diameter, Muraenesocidae : many oil gbobules,2.00-2.46 mm diameter.