Reproductive sturucture of anguilloid fishes as revealed by genome typing for their

通过基因组分型揭示鳗鱼的生殖结构

• 批准号: 15580165
• 负责人: MOCHIOKA Noritaka
• 金额: $ 2.43万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580165/
• 关键词: Anguilliformes; mitochondrial DNA; 16SrRNA; Egg; Muraenesocidae; Ophichthidae; Nettastomatidae; Muraenidae; Ohichthidae; ウナギ目魚類

During 2003-04,a total of 171 net sampling were made waters off Hyuganada, east Kyushu. These sampling ware designed to collect anguilloid eggs. Samplings were made with 1.6 m diameter ring net constructed with 0.5 mm mesh using four layers towing methods (0,5,15,30m depth). Sampling effort was most intensive during summer, however, monthly samples were taken around year. A total 1,215 eggs comprising 19 species were taken. Families represented include the Muraenidae, Ophichthidae, Congridae, Muraenesocidae, and Nettastomatidae from the morphological characteristics, for example, egg diameter, oil globule, pigmentation and number of myomeres of newly hatched larvae. To determine species level identification, mitochondrial DNA(16SrRNA) sequences of those eggs were compared those of adults. Twenty five species were recoginaized from those 19 types eggs. Most species showed highly seasonal patterns of abundance during summer. Based on the present study, following major characters of eggs are revealed ; Muraenidae : absent or few oil globules,2.30-5.50 mm diameter ; Ophichthidae : absent or many oil globules, 1.62-4.40 mm diameter ; Nettastomatidae : absent or two oil globules, 1.98-3.24 mm diameter, Muraenesocidae : many oil gbobules,2.00-2.46 mm diameter.

2003年4月期间，在九州东部Hyuganada海域总共进行了171次净采样。这些采样器皿旨在收集鳗鱼卵。采样采用四层拖曳法（0、5、15、30m 深度），采用 0.5 mm 网眼构成的 1.6 m 直径环网进行。夏季的采样工作最为密集，但全年每月都会采集样本。共取走 19 个品种 1,215 个蛋。从形态特征来看，例如卵直径、油球、色素沉着和新孵化幼虫的肌节数，代表科包括鼠科、蛇科、Congridae、Muraenesocidae 和 Nettastomatidae。为了确定物种水平的鉴定，这些卵的线粒体 DNA (16SrRNA) 序列与成虫的线粒体 DNA (16SrRNA) 序列进行了比较。从这 19 种蛋中识别出了 25 个物种。大多数物种在夏季表现出高度季节性的丰度模式。根据目前的研究，揭示了鸡蛋的以下主要特征；鼠科：无油球或很少，直径2.30-5.50毫米；蛇尾科：无油球或有较多油球，直径1.62-4.40毫米； Nettastomatidae ：无油球或两个油球，直径 1.98-3.24 mm，Muraenesocidae ：许多油球，直径 2.00-2.46 mm。