Population structure and recruitment mechanism of Muraenesox cinereus leptocephali using otolith microsturucture and mitochondrial DNA sequences

利用耳石微结构和线粒体 DNA 序列研究 Muraenesox cinereus leptocephali 的种群结构和招募机制

• 批准号: 11660190
• 负责人: MOCHIOKA Noritaka
• 金额: $ 2.37万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660190/
• 关键词: Muraenesox cinereus; Leptocephalus larva; Anguilliformes; Otolith microstructure; Mitochondrial DNA; Control region; Genetic variability; ミトコンドリアDNA; D-loop; ラベリング

The growth history and recruitment of Muraenesox cinereus were studied, based on growth increments in sagittal otoliths of leptocephali collected from Nobeoka (Miyazaki Pref.), Yatsushiro (Kumamoto Pref.), Onomichi (Hlroshima Pref.), Kamakura (Kanagawa Pref.) and off Goto Islands (East China Sea) in 1999 and in 2000. The periodicity of increment formation was confirmed by treating leptocephali with alizarin complexon and examining otoliths after a known time period. The formation rhythm of otolith increment was about 0.13/day in leptocephalus stage, 0.5/day in metamorphic stage and 1/day in juvenile stage, respectively. Examination of otoliths showed that leptocephali recruiting to Nobeoka Bay devided into two age groups, 150-200 and 250-300 days old. Back calculated hatching dates were distributed throughout most of the year, with a peak in summer and winter.Mitochondrial DNA sequences that include a part of the cytochrome b gene to the first half of the control region of Muraenesox cinereus leptocephali were determined to evaluate the population structure of the species. A total of 591 nucleotide site, including cytochrome b gene to the first half of the control region, was sequenced from specimens from Nobeoka, Yatsushiro, Kamakura and East China Sea. Out of 396 nucleotide sites on the control region, 31 (7.8%) variable, 16 haplotypes being found. Differences between nucleotide sequences ranged between 0.25 and 4.55% with average of 1.88%, indicating that the control region of the mitochondrial DNA in the species is characterized be high variability. The phylogenetic tree constructed by the neighbor-joining method shows neither significant geographic clusters. This result indicate that the present specimen might come from a single population.

根据采集自Nobeoka(Miyazaki Pref.)、Yatsushiro(Kumamoto Pref.)、Oonomichi(Hlroshima Pref.)、镰仓(Kamakura(Kanagawa Pref.)1999年和2000年在后藤岛(东中国海)附近海域。用茜素络合子治疗小头症，在一段已知时间后检查耳石，证实了增量形成的周期性。耳石增量的形成节律在头颈期约为0.13个/天，变态期约为0.5个/天，幼龄期约为1个/天。耳石检查表明，新冈湾的幼鱼分为150~200日龄和250~300日龄两个年龄组。反算出的孵化期分布在全年的大部分时间，以夏季和冬季为高峰。通过测定含有细胞色素b基因前半部分的线粒体DNA序列来评价该物种的种群结构。对取自Nobeoka、Yatsushiro、Kamakura和东中国海的标本进行了591个核苷酸序列测定，其中包括控制区前半部分的细胞色素b基因。在控制区的396个核苷酸位点中，31个(7.8%)为变异，发现16个单倍型。核苷酸序列差异在0.25~4.55%之间，平均为1.88%，表明该物种线粒体DNA控制区具有较高的变异性。用邻接法构建的系统发育树没有显示出显著的地理聚类群。这一结果表明，目前的标本可能来自单个种群。