Respiratory burst activity of ayu (Plecoglossus altivelis) neutrophils : comparison with other fresh water fish

香鱼（Plecoglossus altivelis）中性粒细胞的呼吸爆发活动：与其他淡水鱼的比较

• 批准号: 15580173
• 负责人: MORITOMO Tadaaki
• 金额: $ 2.3万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580173/
• 关键词: ayu; neutrophil; reactive oxygen species; carp; fish; priming; 炎症; NDDPHオキシダーゼ; フグ; ニジマス; 炎症性サイトカイン; NADPH酸化酵素

Neutrophils of vertebrates undergo a respiratory burst activity (RBA) as a defense mechanism against bacterial infections. We report here that ayu (Plecoglossus altivelis) have unusually high RBA even when they are in a healthy condition. Kidney (major hematopoietic organ in teleost fish) and blood leukocytes were obtained from ayu, rainbow trout (Oncorhynchus mykiss), carp (Cyprinus carpio), eel (Anguilla japonica), and pond smelt (Hypomesus nipponensis). Neutrophil RBA was measured by flow cytometry using dihydrorhodamine after stimulation with phorbol myristate acetate (PMA). The amount of RBA of neutrophils from both blood and kidney was significantly higher in ayu than in the other species (e.g., the fluorescence intensity of ayu blood neutrophils was about 3 to 7 times higher than that from trout and carp, and that of ayu kidney neutrophils was 2 to 19 times higher than that of rainbow-trout, carp, eel, and pond-smelt). This unique character of ayu neutrophils was invariable even … More at different ages, locations, and sex-maturation stages.To understand this unique character of ayu neutrophils, the RBAs of resting and inflammatory neutrophils of ayu and carp were compared. Inflammation was induced in the peritoneal cavity by injecting killed-bacteria. The inflammatory neutrophils were obtained from peritoneal cavity. Resting neutrophils were obtained from blood and kidney of non-injected fish.In carp, the RBA of inflammatory neutrophils was much higher than that of resting neutrophils. On the other hand, in ayu no significant difference was observed. The RBA of neutrophils was already high in the kidney stock. The process of inflammation did not further enhance RBA. In addition to PMA, other stimulants (zymosan, opsonized-zymosan, and zymosan treated serum) were used to measure RBA. Even with these stimulants, the RBA of inflammatory neutrophils was always higher than that of kidney neutrophils in carp. On the other hand, no significant difference was observed in ayu. These results indicate ayu neutrophils have spontaneously activated characteristics with the respect to the ROS generation even in the resting state.At this point, it is not clear why ayu have such unique neutrophils with respect to the ROS generation. One possibility is that the high respiratory burst activity is related to the short life span of ayu (essentially one year). With such a short life span, it is likely that the innate immunity by spontaneously activated neutrophils is more important than acquired immunity. Less

脊椎动物的中性粒细胞经历呼吸爆发活动（RBA）作为抵抗细菌感染的防御机制。我们在这里报告，香鱼（Plecoglossus altivelis）有异常高的RBA，即使在他们在健康的条件下。肾脏（硬骨鱼的主要造血器官）和血液白细胞从香鱼、虹鳟鱼（Oncorhynchus mykiss）、鲤鱼（Cyprinus carpio）、鳗鱼（Anguilla japonica）和池塘胡瓜鱼（Hypomesus nipponensis）中获得。在用佛波醇肉豆蔻酸酯乙酸酯（PMA）刺激后，使用二氢罗丹明通过流式细胞术测量神经元RBA。来自血液和肾脏的中性粒细胞的RBA量在香鱼中显著高于其他物种（例如，香鱼血液中性粒细胞的荧光强度比鳟鱼和鲤鱼高约3 - 7倍，香鱼肾脏中性粒细胞的荧光强度比虹鳟、鲤鱼、鳗鱼和池塘胡瓜鱼高2 - 19倍。香鱼中性粒细胞的这一独特特征即使在 ...更多信息 为了了解香鱼中性粒细胞的这种独特性，我们比较了香鱼和鲤鱼的静息和炎症中性粒细胞的RBA。通过注射灭活细菌在腹腔中诱导炎症。从腹腔获得炎性中性粒细胞。静息中性粒细胞取自非注射鱼的血液和肾脏，鲤鱼中炎性中性粒细胞的RBA远高于静息中性粒细胞。另一方面，在香鱼中没有观察到显著差异。在肾脏储备中，中性粒细胞的RBA已经很高。炎症过程没有进一步增强RBA。除PMA外，还使用其他刺激物（酵母聚糖、调理酵母聚糖和酵母聚糖处理的血清）来测量RBA。即使在这些刺激剂的作用下，鲤鱼的炎性中性粒细胞的RBA始终高于肾脏中性粒细胞。另一方面，香玉中没有观察到显着差异。这些结果表明，香鱼的中性粒细胞即使在静止状态下也具有活性氧生成的自发性，但尚不清楚为什么香鱼的中性粒细胞具有如此独特的活性氧生成特性。一种可能性是，高呼吸爆发活动与香鱼的短寿命（基本上是一年）有关。由于寿命如此之短，很可能自发激活的中性粒细胞的先天免疫比获得性免疫更重要。少

项目成果

Rahman, M.H., M.Ototake, T.Nakanishi: "Water soluble adjuvants enhance the protective effect of anti Flavobacterium psychrophilum vaccines in ayu, Plecoglossus altivelis."Fish Pathology. 38(4). 171-176 (2003)

Rahman, M.H.、M.Ototake、T.Nakanishi：“水溶性佐剂增强抗嗜冷黄杆菌疫苗对香鱼、Plecoglossus altivelis 的保护作用。”鱼类病理学。

Comparison of respiratory burst activity of inflammatory neutrophils in ayu (Plecoglossus altivelis) and carp (Cyprinus carpio)

香鱼 (Plecoglossus altivelis) 和鲤鱼 (Cyprinus carpio) 炎症性中性粒细胞呼吸爆发活性的比较

• 发表时间: 2005
• 期刊: Fish & Shellfish Immunology 19
• 作者: Ken Serada;Tadaaki Moritomo;Kyosuke Teshirogi;Takuya Itou;Takashi Shibashi;Yuuki Inoue;Teruyuki Nakanishi
• 通讯作者: Teruyuki Nakanishi

Yuuki INOUE, Yuuki SUENAGA, Yasutoshi YOSHIURA, Tadaaki MORITOMO, Mitsuru OTOTAKE, Teruyuki NAKANSHI: "Molecular Cloning and Sequencing of Japanese pufferfish (Takifugurubripes) NADPH oxidase cDNAs"Developmental & Comparative Immunology. In press.

Yuuki INOUE、Yuuki SUENAGA、Yasutoshi YOSHIURA、Tadaaki MORITOMO、Mitsuru OTOTAKE、Teruyuki NAKANSHI：“日本河豚 (Takifugurubripes) NADPH 氧化酶 cDNA 的分子克隆和测序”发育

Comparison of respiratory burst activity of inflammatory neutrophils in ayu (Plecoglossus altivelis) and carp (Ciprinus carpio)

香鱼 (Plecoglossus altivelis) 和鲤鱼 (Ciprinus carpio) 炎症性中性粒细胞呼吸爆发活性的比较

• 发表时间: 2005
• 期刊: Fish & Shellfish Immunology 19
• 作者: Ken Serada;Tadaaki Moritomo;Kyosuke Teshirogi;Takuya Itou;Takashi Shibashi;Yuuki Inoue;Teruyuki Nakanishi
• 通讯作者: Teruyuki Nakanishi

Tadaaki Moritomo, Naoko Asakura, Mayuko Sekiya, Mituru Ototake, Yuuki Inoue, Teruyuki Nakanishi: "Cell culture of clonal ginbuna crucian carp hematopoietic cells : differentiation of cultured cells into erythrocytes in vivo"Developmental & Comparative imm

Tadaaki Moritomo、Naoko Asakura、Mayuko Sekiya、Mituru Ototake、Yuuki Inoue、Teruyuki Nakanishi：“克隆银鲫鱼造血细胞的细胞培养：培养细胞在体内分化为红细胞”