Characterization of teleost hematopoietic stem cells

在硬骨鱼类中，肾脏是主要的造血器官，其中观察到所有造血分化系，包括红细胞生成、粒细胞生成和淋巴细胞生成。然而，关于肾脏中的造血干细胞或祖细胞的信息很少。本研究采用从三倍体鱼（Carassius auratus langsdorfii）中获得的造血干细胞移植到四倍体鱼（Ginbuna-Gold fish hybrids）中，研究了Ginbuna（Carassius auratus langsdorfii）和Ginbuna-Gold fish hybrids（Ginbuna-Gold fish hybrids）中造血干细胞的存在。我们发现供者来源的三倍体血细胞包括红细胞、血小板、粒细胞和淋巴细胞在受者体内超过18个月。这些结果表明，长期自我更新和多潜能的造血干细胞存在于供体肾脏。我们还分离出的HSCs从金枪鱼和斑马鱼（Danio rerio）肾脏侧群（SP）分析的基础上Hoechst染料排除。通过Ginbuna SP细胞在体内的长期再增殖和多向分化潜能来评价其HSC活性。

In teleosts, kidney is the major hematopoietic organ in which all lines of hematopoietic differentiation are observed including erythropoiesis, granulopoiesis, and lymphopoiesis. However, little information was available about the hematopoietic stem or progenitor cells in the kidney. In the present studies, we demonstrated the presence of hematopoietic stem cells (HSCs) in kidney hematopoietic tissues using the transplantation system of ginbuna (Carassius auratus langsdorfii) and ginbuna-gold fish hybrids.Kidney hematopoietic cells were obtained from ginbuna (triploid fish) and transplanted into ginbuna-gold fish hybrids (tetraploid fish). We found donor derived triploid blood cells including erythrocytes, thrombocytes, granulocytes and lymphocytes in the recipient for more than 18 months. These results indicate that long-term self-renewal and multipotent HSCs are present in the donor kidney.We also isolated HSCs from ginbuna and zebrafish (Danio rerio) kidney using side population (SP) analysis on the basis of Hoechst dye exclusion. The HSC activity of ginbuna SP cells were evaluated by their potential for long-term repopulation and multilineage differentiation in vivo.