The cultivation method of healthy marine fish seedlings using the heat shock factor as an indicator

以热激因子为指标的健康海水鱼苗培育方法

• 批准号: 15580174
• 负责人: ISHIBASHI Yasunori
• 金额: $ 1.98万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580174/
• 关键词: environmental stress tolerance; heat shock factor; larvae and juvenile; healthy fish seedlings; Marine fish; cortisol; glucocorticoid receptor; 建苗性; ストレスタンパク質; グルココルチゾルレセプター; マダイ

Some indicators of environmental stressors were examined in the development of healthy fish seedling production. In addition, ontogenic changes in the stress response to environmental stressors were studied in some kinds of fish in their lifespan from larvae through the juvenile stage. At first, Pagrus major juveniles were subjected to low oxygen, high and low temperatures, hypo-and hyper-salinity. The specific activities of heat shock factor (HSF), glucocorticoid receptor (GR) and cortisol level as stress indicators in the fish were compared. The cortisol concentration was increased just after stress exposure, but the specific activities of HSF and GR gradually tended to become elevate. Fish showing both high and low tolerance to stress exposure were also selected and determined to be stress indicators. HSF and GR activities in high tolerance fish tended to be higher than those in the low tolerance fish. The HSF, GR and cortisol level were suggested to be good indicators to check in fish. Next, the ontogeny of hypoxia tolerance and stress response were studied in Pagrus major, Paralichthys olivaceus, Takifugu rubripes and Thunnus orientalis larvae and juveniles. The results indicated that around the stages of flexion and post-flexion larvae in fish, metabolic rates and/or energy metabolism were the highest, and consequently hypoxia tolerance was the lowest. Finally, ontogenic changes in some stress tolerances and the response to this were examined in larvae and juveniles. It was found that at the early juvenile stage all of the tolerances and the cortisol level were increased. The effects of cortisol immersion on various stress tolerances in larvae were also tested. The response of the survival rate, cortisol concentration and GR activity to stressors showed a tendency to increase as a result of cortisol immersion. It was therefore suggested that cortisol has an important role in adaptation to environmental stressors.

研究了环境应激源的一些指标在健康鱼苗生产发展中的作用。此外，个体发育的变化，在应激反应的环境应激源进行了研究，在某些种类的鱼在其寿命从幼虫到少年阶段。真鲷幼鱼首先经历低氧、高温和低温、低盐和高盐环境。比较了热休克因子（HSF）、糖皮质激素受体（GR）和皮质醇水平作为应激指标在鱼体内的比活性。应激后皮质醇浓度升高，HSF和GR比活性逐渐升高。还选择了对应激暴露表现出高和低耐受性的鱼，并将其确定为应激指标。高耐受性鱼的HSF和GR活性高于低耐受性鱼。HSF、GR和皮质醇水平可作为检测鱼类的良好指标。其次，对真鲷、褐牙鲆、红鳍东方鲀和东方金枪鱼仔稚鱼的耐缺氧能力和应激反应的个体发生进行了研究。结果表明，在前屈期和后屈期前后，鱼的代谢率和能量代谢最高，耐缺氧能力最低。最后，个体发育的变化，在一些压力的耐受性和对此的反应进行了研究，在幼虫和少年。结果发现，在早期的少年阶段，所有的耐受性和皮质醇水平增加。皮质醇浸泡对幼虫各种应激耐受力的影响也进行了测试。存活率、皮质醇浓度和GR活性对应激的反应表现出增加的趋势。因此，这表明皮质醇在适应环境应激源中具有重要作用。

项目成果

Stress and the recovery from stress status in cultivated fish.

养殖鱼类的应激和应激状态的恢复。

• 发表时间: 2003
• 期刊: Aquanet 6(10)
• 作者: Ishibashi Y;Ozawa M;Hirata H;Kumai H. Ontogenic;Ishibashi Y.
• 通讯作者: Ishibashi Y.

養殖魚の環境変化に対するストレス反応と「慣れ」

养殖鱼类对环境变化的应激反应和“习惯”

• 发表时间: 2006
• 期刊: アクアネット 9・5
• 作者: Ken Serada;Tadaaki Moritomo;Kyosuke Teshirogi;Takuya Itou;Takashi Shibashi;Yuuki Inoue;Teruyuki Nakanishi;石橋泰典
• 通讯作者: 石橋泰典

養殖魚のストレスと回復のための間

养殖鱼类的应激和恢复期间

• 发表时间: 2003
• 期刊: アクアネット 6・10
• 作者: 石橋泰典

石橋泰典, 小澤 勝, 平田八郎, 熊井英水: "マダイPagrus maior仔稚魚の発育に伴う各種環境ストレス耐性の変化"日本水産学会誌. 69・1. 36-43 (2003)

石桥康典、小泽胜、平田八郎、熊井秀美：“与幼鱼真鲷发育相关的各种环境胁迫耐受性的变化”日本水产学会杂志 69・1（2003 年）。

changes in various stress tolerances of larval and juvenile red sea bream Pagrus major.

真鲷幼鱼和幼鱼的各种应激耐受力的变化。

• 发表时间: 2003
• 期刊: Nippon Suisan Gakkaishi 69(1)
• 作者: Ishibashi Y;Ozawa M;Hirata H;Kumai H. Ontogenic
• 通讯作者: Kumai H. Ontogenic