ANALYSIS OF ADHESIVE AND INVASION MECHANISM OF PIROPLASMA IN VECTOR TICK

1.研究了针刺法将马巴贝斯虫(巴贝斯虫)和马巴贝斯虫(巴贝斯虫)感染转移到长角血吸虫体内的有效性。此外，我们还确定了被调查的这些寄生虫在这只扁虱中的经卵管和经传播。用皮下注射针将充血的成虫或若虫从被膜向中肠注射卡巴氏杆菌或马巴虫感染的红细胞。用套式聚合酶链式反应检测硬蜱体内马巴贝斯虫的寄生虫DNA。在经卵传播方面，在成虫、产卵和幼虫中分别检测到卡氏巴氏杆菌和马巴氏杆菌DNA。对于经性传播，在注射若虫蜕皮后的成虫体内和注射若虫蜕皮后的成虫唾液腺和身体组织中都检测到了马巴斯虫DNA。2.我们建立了针对马巴贝斯虫表面蛋白和胞外裂殖子mAb的单抗，并将其用于筛选从巴贝斯虫裂殖子mRNA中制备的高表达蛋白的cDNA表达文库。3.实时荧光定量聚合酶链式反应检测马巴贝斯虫感染中寄生虫DNA的重复性和准确性，且远比传统的显微镜检查敏感。LAMP法为检测犬吉氏芽孢杆菌感染提供了一种有用的工具。4.Apo-LF对卡氏芽孢杆菌的抑制作用最强，这可能是由于培养基中的一种生长因子失活或抑制所致。

1.We investigated the usefulness of the needle injection method as an experimental means of transferring infection of equine Babesia parasites, namely Babesia caballi and Babesia equi, to the tick Haemapysalis longicornis. Further, we also determine investigated the transovarial and transstadial transmission of these parasites in this tick. Engorged adult or nymph ticks were injected with B.caballi- or B.equi-infected erythrocytes into the midgut from the integument by hypodermic needle. Detection of equine Babesia in ticks was by nested PCR for parasite DNA. For transovarial transmission, B.caballi and B.equi DNA were detected in adult ticks, laid eggs and larvae, respectively. For transstadial transmission, B. equi DNA was detected in adult ticks after molt of the injected nymph, and in salivary glands and carcass tissues of adult ticks after molt of the injected nymph. These findings support the potential role of H.longicornis in the transmission of equine Babesia.2.We established mAbs against the Babesia surface protein and extracellular merozoite termed mAb, and used it to screen cDNA expression library prepared from Babesia merozoite mRNA for highly expressed proteins.3.Real time-PCR was found to be reproducible and accurate for the quantification of parasite DNA in experimentally infection and far more sensitive than traditional microscopic examination. LAMP method provides a useful tool for the detecton of B.gibsoni infection in dog.4.Apo-LF had the strongest inhibitory effect on B.caballi ; this may have been caused by inactivation or inhibition of a growth factor in the culture medium.