ANALYSIS OF ADHESIVE AND INVASION MECHANISM OF PIROPLASMA IN VECTOR TICK

载体蜱中焦原体的粘附和侵袭机制分析

基本信息

批准号：
15580265
负责人：
OYAMADA Takashi
金额：
$ 2.43万
依托单位：
KITASATO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

1.We investigated the usefulness of the needle injection method as an experimental means of transferring infection of equine Babesia parasites, namely Babesia caballi and Babesia equi, to the tick Haemapysalis longicornis. Further, we also determine investigated the transovarial and transstadial transmission of these parasites in this tick. Engorged adult or nymph ticks were injected with B.caballi- or B.equi-infected erythrocytes into the midgut from the integument by hypodermic needle. Detection of equine Babesia in ticks was by nested PCR for parasite DNA. For transovarial transmission, B.caballi and B.equi DNA were detected in adult ticks, laid eggs and larvae, respectively. For transstadial transmission, B. equi DNA was detected in adult ticks after molt of the injected nymph, and in salivary glands and carcass tissues of adult ticks after molt of the injected nymph. These findings support the potential role of H.longicornis in the transmission of equine Babesia.2.We established mAbs against the Babesia surface protein and extracellular merozoite termed mAb, and used it to screen cDNA expression library prepared from Babesia merozoite mRNA for highly expressed proteins.3.Real time-PCR was found to be reproducible and accurate for the quantification of parasite DNA in experimentally infection and far more sensitive than traditional microscopic examination. LAMP method provides a useful tool for the detecton of B.gibsoni infection in dog.4.Apo-LF had the strongest inhibitory effect on B.caballi ; this may have been caused by inactivation or inhibition of a growth factor in the culture medium.

1.我们研究了针喷射法的有用性，作为转移马辣椒寄生虫感染的一种实验手段，即巴贝西亚·卡巴利（Babesia caballi）和贝贝西亚·埃科（Babesia Equi），向tick虫haemapysalis longicornis转移了感染。此外，我们还确定了这些寄生虫在此壁虱中研究了这些寄生虫的跨型和跨性传播。将成人或若虫的壁虱注入了b.caballi-或B. Equi感染的红细胞通过皮下注射针头中的中肠中的中肠。 tick中的马贝西亚检测是通过嵌套的PCR用于寄生虫DNA。对于分流传播，分别在成人壁虱，卵子和幼虫中检测到b.caballi和b.equi DNA。对于跨性传播，在注射若虫的蜕皮后，在成年tick中检测到B. equi DNA，并在注射的若虫蜕皮后，在成年tick虫的唾液腺和car体组织中检测到。 These findings support the potential role of H.longicornis in the transmission of equine Babesia.2.We established mAbs against the Babesia surface protein and extracellular merozoite termed mAb, and used it to screen cDNA expression library prepared from Babesia merozoite mRNA for highly expressed proteins.3.Real time-PCR was found to be reproducible and accurate for the quantification of parasite DNA in experimentally infection and far more比传统的微观检查敏感。 LAMP方法为狗中b.gibsoni感染的检测提供了有用的工具。4。APO-LF对b.caballi具有最强的抑制作用。这可能是由于培养基中的生长因子的灭活或抑制引起的。

项目成果

期刊论文数量（44）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Molecular characterization of a putative protein disulfide isomerase from Babesa caballi.

来自 Babesa caballi 的推定蛋白质二硫键异构酶的分子表征。

DOI：
发表时间：
2005
期刊：
Parasitology 131・Pt6
影响因子：
0
作者：
Ikadai;H.;Tsukada;R.(その他6名)
通讯作者：
R.(その他6名)

Incidence of canine Babesia gibsoni infection and subclinical infection among Tosa dog in Aomori Prefecture, Japan.

日本青森县土佐犬吉氏巴贝虫感染及亚临床感染的发生率。

DOI：
发表时间：
2004
期刊：
J.Vet.Med.Sci. 66
影响因子：
0
作者：
Matsuu;A.;Kawabe;A.;Koshida;Y.;Ikadai;H.;Okano;S.;Higuchi;S.
通讯作者：
S.

Inhibitory effect of lactoferrin on in vitro grouth of Babesia caballi.

乳铁蛋白对马贝斯虫体外生长的抑制作用。

DOI：
发表时间：
2005
期刊：
Amercan Journao of tropical Medicine and Hygine 73・4
影响因子：
0
作者：
Ikadai;H.;Tanaka;T.(その他7名)
通讯作者：
T.(その他7名)

Inhibitory effect of lactoferrin on in vitro growth of Babesia cuballi.

乳铁蛋白对古巴巴贝虫体外生长的抑制作用。

DOI：
发表时间：
2005
期刊：
Am.J.Trop.Med.Hyg. 73
影响因子：
0
作者：
Ikadai;H.;Tanaka;T.;Shibahara;N.;Tanaka;H.;Matsuu;A.;Kudo;N.;Shimazaki;K.;Igarashi;I.;Oyamada;T.
通讯作者：
T.

Inhibitory effect of lactoferrin on the in vitro growth of Babesia caballi protozoan.

乳铁蛋白对巴贝斯虫原生动物体外生长的抑制作用。