Molecular Biological Study of Species Specific Carcinogenicity of Food Red No.2 (amaranth)

食品红2号（苋菜）种属特异性致癌性的分子生物学研究

• 批准号: 15580271
• 负责人: TSUDA Shuji
• 金额: $ 2.37万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580271/
• 关键词: DNA damage; comet method; food additives; food red 2; carcinogenicity; DNAの損傷

The data concerning to the species specific carcinogenicity of food red 2 were as follows :1.DNA damage in colon mucous membrane of mice induced by food red 2 by ip injection was less prominent than po administration.2.That DNA damage in colon disappeared when the bile legation was applied before po administration.3.That DNA damage in colon was observed where the food red 2 reached.4.Colon strips Isolated from either rats or mice showed DNA damage, when incubated with colon content of mice after oral administration of food red 2.From these results, the difference between rats and mice in DNA damage of colon is suggested to be due to the difference in food red 2 metabolism between the two species.Using HPLC we identified a specific peak in the colon content of mice after oral administration of food red 2.After the 5 days multiple administration of food red 2, colon DNA damage in mice disappeared, which may be the decrease of sensitivity of cells in colon mucous membrane.In order to more precisely extrapolate rodent data to humans concerning the carcinogenicity of food red 2, identification of food red 2 metabolite in rats would be necessarily.

有关食用红2种属特异性致癌性的数据如下：1.腹腔注射食物红2对小鼠结肠粘膜的DNA损伤较口服给药明显减轻; 2.口服食物红2后，在给药前进行胆汁提取，结肠粘膜DNA损伤消失; 3.食物红2到达结肠粘膜后，结肠粘膜DNA损伤明显; 4.小鼠口服食物红2后，结肠粘膜DNA损伤明显。大鼠和小鼠结肠DNA损伤的差异可能是由于两种动物在食物红2代谢方面的差异所致。小鼠经口给予食用红2后，结肠DNA损伤消失，可能是结肠粘膜细胞敏感性降低，为了更准确地将啮齿类动物有关食用红2致癌性的数据外推到人类，有必要鉴定食用红2在大鼠体内的代谢产物。