权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Nasal immunization with mature sperm specific antigen CD52 for developing a contraceptive vaccine

使用成熟精子特异性抗原 CD52 进行鼻免疫以开发避孕疫苗

基本信息

批准号：
15590147
负责人：
AKATANI AKIKO
金额：
$ 2.05万
依托单位：
Hyogo College of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590147/
关键词：
sperm antigen carbohydrate epitope of CD52 GPI anchor protein immuno-contraceptive vaccine nasal immunity fertilization blockin infertility 卵透明帯受精 CD52 精巣上体粘膜免疫

项目摘要

Human CD52 has been reported to be a glycosylphosphatidylinositol anchor protein that is present in lymphocytes, monocytes, male reproductive tracts, ejaculated sperm and seminal plasma. This antigen induces antibodies causing infertility in women and impairs sperm motility. The present study is conducted as a model experiment to examine whether mouse mrt-CD52 produces antibodies in mice to clarify mer-CD52 antigen cause infertility and also this antigen is available for contraceptive vaccine development. [Methods] mrt-C52 was prepared from ICR mouse vas deferens and injected in male and female C57BL mice with complete Freund's adjuvant. Alternatively, mice were immunized by intranasal route with cholera toxin B. Antibody production was assessed by ELISA, western blotting and immunofluorescent staining. Biological effects of the antibodies were examined by sperm immobilizing test, in-vitro fertilization and mating experiments. [Results] Male and female mice produced auto- and iso-antibodies by immunization with purified mouse mrt-CD52 by a subcutaneous route. All the produced antibodies did not react to the peptide portion of mrt-CD52, suggesting that the carbohydrate moiety is immunogenic. The antibodies immobilized mouse sperm in the presence of complement but not inhibited in-vitro fertilization or in-vivo fertility. [Discussion] The results suggested that the antibody to mrt-C52 possibly cause infertility in some infertile women. Further experiment is necessary to produce IgA class antibody in female genital tracts for developing a contraceptive vaccine by mucosal immunization with mrt-CD52.

人CD52是一种糖基化磷脂酰肌醇锚蛋白，存在于淋巴细胞、单核细胞、男性生殖道、射精和精浆中。这种抗原诱导产生抗体，导致女性不育，并损害精子的活力。本研究旨在探讨小鼠MRT-CD52在小鼠体内是否产生抗体，以阐明mer-CD52抗原是否可导致不孕症，以及该抗原是否可用于避孕疫苗的研制。[方法]从ICR小鼠输精管制备MRT-C52，以完全弗氏佐剂注射于雄性和雌性C57BL小鼠体内。用霍乱毒素B滴鼻免疫小鼠，用ELISA法、免疫印迹法和免疫荧光法检测抗体产生情况。通过精子固定试验、体外受精和交配实验检测抗体的生物学效应。[结果]用纯化的小鼠MRT-CD52皮下免疫雌雄小鼠，产生自身抗体和同种抗体。所有产生的抗体都不与MRT-CD52的多肽部分发生反应，表明该糖类部分具有免疫原性。该抗体在补体存在的情况下固定小鼠精子，但不抑制体外受精或体内受精。[讨论]提示MRT-C52抗体可能导致部分不孕症妇女不孕。进一步的实验需要在女性生殖道产生IgA类抗体，以开发MRT-CD52粘膜免疫避孕疫苗。