Analysis of the receptor tyrosine kinase genes in the human solid cancers and its clinical application

免疫组化（IHC）和荧光原位杂交（FISH）分别检测ErbB 2、EGFR和Met等酪氨酸激酶生长因子受体的蛋白过表达和基因扩增。使用购自Vysis，Inc.的荧光标记DNA探针组，确定是否存在c-erbB-2（染色体位点17q11.2-q12）、EGFR（7 p12）和c-met（7 q31）的基因扩增。结果如下：大肠癌ErbB-2基因在244例大肠癌中有8例（3%）过度表达，且均存在基因扩增。在244例肿瘤中，19例（8%）观察到EGFR过表达，其中58%的肿瘤存在基因扩增。181例非小细胞肺癌中EGFR蛋白的过表达率为34%。其中，EGFR扩增被证明在74%。高水平的基因扩增仅在高蛋白表达的癌细胞中发现， ...更多信息 胆道癌221例，其中肝内胆管癌28例，肝外胆管癌78例，胆囊癌89例，壶腹癌26例。ErbB-2在胆囊癌、壶腹癌和肝外胆管癌中的表达率分别为15.7%、11.5%和5.1%，其中79%与基因扩增有关。EGFR的过度表达在8.1%的肿瘤中被发现，没有主要的位置，并且也与高频率的基因扩增（77%）相关。106例食管鳞癌中53例（50%）EGFR蛋白过表达，15例有EGFR基因扩增，32例EGFR拷贝稍高，6例无扩增。基因扩增与高水平过表达显著相关。少

Protein overexpression and gene amplification of the tyrosine kinase growth factor receptors such as ErbB2, epidermal growth factor receptor (EGFR) and Met by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) respectively. The presence or absence of gene amplification of c-erbB-2 (chromosomal locus 17q11.2-q12), EGFR (7p12) and c-met (7q31) was determined using fluorescently labeled DNA probe sets purchased from Vysis, Inc. The results are as follows :Colorectal carcinomas Overexpression of ErbB-2 was found in 8 (3%) of 244 colorectal carcinomas and all of them had gene amplification. Overexpression of EGFR was observed in 19 (8%) of the 244 tumors, and gene amplification was found in 58% of them. Non-small cell lung cancers Overexpression of EGFR protein was found in 34% of 181 cases of non small cell lung cancers. Among these, EGFR amplification was demonstrated in 74%. High level gene amplification was found exclusively in cancer cells with high protein expres … More sion.Bile duct carcinomas A total 221 biliary tract carcinomas consisting of 28 intrahepatic bile duct, 78 extrahepatic bile duct, 89 gallbladder and 26 ampulla of Vater were examined. Overexpression of ErbB-2 was found in 15.7%, 11.5%, and 5.1% of carcinomas of the gallbladder, ampulla of Vater, and extrahepatic bile duct, respectively, and 79% of them were associated with gene amplification. Overexpression of EGFR was found in 8.1% of tumors with no predominant location and also associated with gene amplification in high frequency (77%). Met overexpression, most frequent in intrahepatic bile duct carcinomas (21.4%), was not associated with gene amplification.Esophageal squamous cell carcinomas EGFR protein is overexpressed in 53 (50%) of the 106 esophageal squamous cell carcinomas examined EGFR gene amplification was observed in 15 of the 53 tumors, somewhat higher EGFR copy in 32 cases, and no increase in 6 cases. Gene amplification was significantly associated with high level overexpression. Less