BIOLOGICAL CHARACTERISTICS OF PRIMARY MAMMARY MALIGNANT LYMPHOMA

我们分析了I期和II期乳腺弥漫性大B细胞淋巴瘤(DLBCL)，以揭示DLBCL预后较差的原因。石蜡切片免疫组织化学：淋巴瘤细胞免疫表型：CD3；0/15(阳性病例/阳性病例)，CD5；2/15，CD10；0/15，CD20；15/15，Bcl2；10/15，Bcl6；5/15，IRF4/MUM1；15/15。MIB-1增殖指数59.5%~95.2%，平均79.2%。免疫球蛋白重链可变区(IgH-V)突变分析：7例患者的突变频率分布在1.43%~10.29%之间。5例未发现持续突变，提示乳腺DLBCL淋巴瘤细胞属于CD10^-Bcl6；+/-&Gt；IRF4/MUM1+非生发中心B细胞DLBCL(ACTIVATED B-cell DLBCL)亚群，具有较高的MIB-1增殖指数。乳腺DLBCL患者预后较差可能与淋巴瘤细胞MIB指数高有关。应用人10K寡核苷酸芯片对4例乳腺DLBCL和4例结节性DLBCL进行基因芯片分析，探讨乳腺DLBCL淋巴瘤细胞具有高MIB-1增殖指数的原因。8例均为女性，年龄均在30岁以上。所有淋巴瘤组织学均为中心母细胞型DLBCL，免疫组织化学显示非生发中心B细胞型DLBCL。发现了两组间差异表达的基因，发现了原发乳腺DLBCL的生物学特性，并继续对这些基因的细节进行分析。有或无Bcl6表达的病例可能是重要的。

We analyzed primary mammary diffuse large B-cell lymphoma (DLBCL), with stage I and II, in order to disclose why primary mammary DLBCL showed a poorer prognosis.1. Immunohistochemistry of the paraffin-embedded section : Immunophenotype of the lymphoma cells is as follows: CD3;0/15 (positive cases/examined cases), CD5; 2/15, CD10; 0/15, CD20; 15/15, Bcl-2; 10/15, Bcl-6; 5/15 and IRF4/MUM1; 15/15. A MIB-1 proliferation index was from 59.5% to 95.2% with an average of 79.2%.2. Mutation analysis of the immunoglobulin heavy chain variable region (IgH-V): Mutation frequency of examined 7 cases distributed from 1.43 to 10.29%. No ongoing mutation was found in examined 5 cases.These results, therefore, indicated that the lymphoma cells of mammary DLBCL belonged to a subgroup of CD10^- Bcl-6^<+/-> IRF4/MUM1^+ non-germinal center B cell DLBCL (activated B-cell DLBCL) and exhibited a high proportion of MIB-1 proliferation index. A poorer outcome of patients with mammary DLBCL may be related to high MIB index of the lymphoma cells.3. cDNA microarray using a human 10K oligotip was analyzed between 4 cases of mammary DLBCL and 4 cases of nodal DLBCL in order to find the reason why the lymphoma cells of mammary DLBCL have a high MIB-1 proliferation index. The 8 patients were all female and more than 30 years old. The lymphoma of all cases showed a centroblastic variant of DLBCL in histology and non-germinal center B cell DLBCL by immunohistochemistry. Some genes having a different expression pattern between two groups were found.We found biological characteristics of primary mammary DLBCL and continue to analyze details of the genes. Cases with or without Bcl-6 expression may be important.