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Functional alteration of glial cells and brain edema formation

神经胶质细胞的功能改变和脑水肿形成

基本信息

批准号：
15590466
负责人：
KOYAMA Yutaka
金额：
$ 2.3万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590466/
关键词：
Glial cells Astrocytes Brain edema Blood-Brain Barrier Endothelial cells

项目摘要

Increases in the permeability of In vitro Blood-Brain Barrier Model by Endothelns : An in vitro models of blood-brain barrier (BBB) using cultured astrocytes and brain capillary endothelial cells (bEnd.3) was made and the effects of endothelins on the permeability of this BBB model was examined. Endothelin-1 increased the permeability of bEnd.3 monolayer culture. RT-PCR analysis showed ETB receptors were expressed in bEnd.3 cells. These results suggest that endothelias are one of soluble factors inducing brain edema on brain pathologies and ETB receptor is a possible target of anti-brain edema drugs.Production of Astrocyte-Derived Edema-Inducing Factors by Endothelins : Astrocyte is a component of BBB together with brain capillary endothelial cells and produces several soluble factors modulating BBB permeability. In cultured astrocytres, ET-1 increases mRNA levels of vascular endothelial cells growth factor (VEGF), monocyte chemoatractant protein -1 (MCP-1) and interleukin-8 (IL-8), which are shown to increases after brain pathologies and induces brain edema Matrixmetalloproteinase-2 and 9 (MMPs) degenerate extracellular matrix proteins, such as laminine and fibronectin, and are involved in angiogenesis after brain injuries. Endothelia-1 increased MMP-2 and 9 in cultured astrocytses. Administration of Ala-ET-1, a selective agonist for ETB receptors, increased expression of VEGF, IL-8, MMP-2 and MMP-9 in rat brain. These results suggest a possibility that endothelins cause brain edema through the production of these edema-inducing factors in astrocytes.

内皮素对体外血脑屏障通透性的影响：采用培养的星形胶质细胞和脑毛细血管内皮细胞（bEnd.3）制备体外血脑屏障（BBB）模型，观察内皮素对血脑屏障通透性的影响。内皮素-1增加了bEnd的通透性。3 .单层培养。RT-PCR分析显示，ETB受体在bEnd中表达。3细胞。这些结果提示内皮细胞是脑病理诱导脑水肿的可溶性因子之一，ETB受体可能是抗脑水肿药物的靶点。星形胶质细胞来源的致水肿因子由内皮素产生：星形胶质细胞与脑毛细血管内皮细胞一起是血脑屏障的组成部分，并产生几种调节血脑屏障通透性的可溶性因子。在培养的星形胶质细胞中，ET-1增加血管内皮细胞生长因子（VEGF）、单核细胞趋化蛋白-1 （MCP-1）和白细胞介素-8 （IL-8）的mRNA水平，这些mRNA水平在脑病变后升高并诱导脑水肿基质金属蛋白酶-2和9 （MMPs）变性细胞外基质蛋白，如层胺和纤维连接蛋白，并参与脑损伤后的血管生成。内皮-1在培养的星形胶质细胞中增加MMP-2和9。给药后，大鼠脑组织中VEGF、IL-8、MMP-2和MMP-9的表达增加。这些结果表明，内皮素可能通过在星形胶质细胞中产生这些致水肿因子而引起脑水肿。

项目成果

期刊论文数量（60）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

SEA0400, a specific inhibitor of Na-Ca exchanger, attenuates sodium nitroprusside-induced apoptosis in cultured astrocytes

SEA0400 是一种 Na-Ca 交换器特异性抑制剂，可减弱硝普钠诱导的培养星形胶质细胞细胞凋亡

DOI：
发表时间：
2005
期刊：
Br.J.Pharmacol. 144
影响因子：
0
作者：
Koyama Y.;Nagano T.
通讯作者：
Nagano T.

The selective Na-Ca exchange inhibitor attenuates brain edema after radiofrequency lesion in rats.

选择性 Na-Ca 交换抑制剂可减轻大鼠射频损伤后的脑水肿。

DOI：
发表时间：
2004
期刊：
Eur J Pharmacol 489
影响因子：
0
作者：
Koyama Y.;Nagano T.;Matsuda T;Matsuda T.;Koyama Y.;Nagano T.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.
通讯作者：
Koyama Y.

新規脳改善薬FK960のGDNF産生の促進

大脑改善新药FK960促进GDNF产生

DOI：
发表时间：
2003
期刊：
Clinical Neuroscience 22
影响因子：
0
作者：
Koyama Y.;Nagano T.;Matsuda T;Matsuda T.;Koyama Y.;Nagano T.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Funakoshi-Tago M.;松田敏夫
通讯作者：
松田敏夫

Endothelia-1 stimulates glial cell line-derived neurotrphic factor (GDNF) expression in cultured astrocytes

Endothelia-1 刺激培养的星形胶质细胞中胶质细胞系源性神经营养因子 (GDNF) 的表达

DOI：
发表时间：
2003
期刊：
Biochem.Biophis Res.Commun. 303
影响因子：
0
作者：
Koyama Y.;Nagano T.;Matsuda T;Matsuda T.;Koyama Y.;Nagano T.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Koyama Y.;Funakoshi-Tago M.;松田敏夫;Funakoshi-Tago M.;Sakaue M;Koyama Y.;Funakoshi-Tago M;Utsubo R;Matsuda T.;Tamagiku Y;Sonoda Y.;Koyama Y.
通讯作者：
Koyama Y.

Koyama Y.: "Focal adhesion kinase is required for endothelin-induced cell cycle progression of cultured astrocyets"Glia. 43. 185-189 (2003)

Koyama Y.：“内皮素诱导的培养星形细胞的细胞周期进程需要粘着斑激酶”Glia。