Effect of thyroid hormone on the production of cytokines from lymphocytes

甲状腺激素对淋巴细胞产生细胞因子的影响

• 批准号: 15590487
• 负责人: HIDAKA Yoh
• 金额: $ 2.3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590487/
• 关键词: Graves' disease; thyroid hormone; Th1; Th2; IFN-γ; IL-4

The value of TSH receptor antibody will fall if thyroid function is normalized in Graves' disease. The duration of destruction is less than three months in destructive thyrotoxicosis. In order to explain these phenomenon, the hypothesis "thyroid hormone suppresses Th1 and stimulates Th2" was examined. Results ; (1) Whole blood was cultured with thyroid hormone (T3), and the amounts of IFN-gamma and IL-4 were measured with Invader RNA Assay. T3 slightly increased IFN-gamma and mildly increased IL-4. (2) Peripheral blood mononuclear cells from four people were cultured with T3, and the amounts of several cytokines were measured with TaqMan Cytokine Gene Expression Plate I. The results were different in every specimen. (3) Whole blood was cultured with PHA and T3, and the amount of IL-4 in a supernatant was measured by ELISA. IL-4 was not changed significantly by T3. (4) Peripheral blood mononuclear cells were cultured with PHA and T3, and the IFN-gamma or IL-4 producing cells were measured by ELISPOT. T3 slightly increased IFN-gamma producing cells, and slightly decreased IL-4 producing cells. Conclusions ; Thyroid hormone did not show a constant change in the Cytokine production from lymphocytes.

Graves病患者甲状腺功能恢复正常后，TSH受体抗体值下降。破坏性甲状腺毒症的破坏持续时间不到三个月。为了解释这些现象，检验了“甲状腺激素抑制Th 1并刺激Th 2”的假设。结果：（1）用甲状腺激素（T3）培养全血，用Invader RNA Assay检测IFN-γ和IL-4的含量。T3轻微增加IFN-γ和轻度增加IL-4。(2)采用TaqMan Cytokine Gene Expression Plate I检测4例受试者外周血单个核细胞中几种细胞因子的表达。不同标本的结果不同。(3)用PHA和T3培养全血，通过ELISA测定上清液中IL-4的量。T3对IL-4无明显影响。(4)用PHA和T3培养外周血单个核细胞，用ELISPOT检测IFN-γ和IL-4产生细胞。T3略微增加产生IFN-γ的细胞，并略微减少产生IL-4的细胞。结论：甲状腺激素在淋巴细胞产生细胞因子方面没有表现出恒定的变化。

项目成果

Increase in serum levels of autoantibodies after attack of seasonal allergic rhinitis in patients with Graves' disease.

格雷夫斯病患者季节性过敏性鼻炎发作后血清自身抗体水平升高。

• 发表时间: 2003
• 期刊: International Archives of Allergy and Immunology 132・3
• 作者: Ishida Tetsuo;Tetsuo Ishida;Atsushi Hirano;Hirohisa Ichikawa;Mikio Kataoka;片岡幹男;Noriko Tagawa;Nobuyuki Amino;Keiko Takeoka
• 通讯作者: Keiko Takeoka

Yukiko Izumi: "Relation between postpartum liver dysfunction and anti-cytochrome 2D6 antibodies"American Journal of Reproductive Immunology. 50・4. 355-362 (2003)

泉由纪子：“产后肝功能障碍与抗细胞色素 2D6 抗体的关系”美国生殖免疫学杂志 50・4（2003）。

Nobuyuki Amino: "No increase of blocking type anti-thyrotropin receptor antibodies during pregnancy in patients with Graves' disease"Journal of Clinical Endocrinology and Metabolism. 88・12. 5871-5874 (2003)

Nobuyuki Amino：“格雷夫斯病患者妊娠期间阻断型抗促甲状腺激素受体抗体没有增加”临床内分泌与代谢杂志88・12（2003）。

Nobuyuki Amino: "Association of seasonal allergic rhinitis is high in Graves' disease and low in painless thyroiditis"Thyroid. 13・8. 811-814 (2003)

Nobuyuki Amino：“季节性过敏性鼻炎与格雷夫斯病的关联性较高，而与无痛性甲状腺炎的关联性较低”，《甲状腺》13・8（2003）。

Serum concentrations of dehydroepiandrosterone and dehydroepiandrosterone sulfate and their relation to cytokine production during and after normal pregnancy.

脱氢表雄酮和硫酸脱氢表雄酮的血清浓度及其与正常妊娠期间和之后细胞因子产生的关系。

• 发表时间: 2004
• 期刊: Clinica Chimica Acta 340
• 作者: Miyoshi;M.;Nadai;M.;Nitta;A.;Ueyama;J.;Shimizu;A.;Takagi;K.;Nabeshima;T.;Takagi;K.;Saito;K.;Hasegawa;T.;Ishida Tetsuo;Noriko Tagawa
• 通讯作者: Noriko Tagawa