Development of monitoring system of colorectal cancer

结直肠癌监测系统的研制

基本信息

批准号：
15590499
负责人：
TANAKA Tomoo
金额：
$ 1.92万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

A purpose of this study is to develop diagnostic technology of colorectal cancer with a carcinoma cell-specific expressed material, Bradeion discovered by our research group newly, and to finally develop new treatment technology including a cancer cell-targeting medicine.In 2003, we demonstrated utility of a Bradeion molecule (a gene and a protein) for a diagnostic technology of a colorectal carcinoma by two independent system; real time RT-PCR to measure the expressed Bradeion gene (during a patent application), and an immunochromatography (test strip method) to detect Bradeion protein of specimens with a monoclonal antibodies (ref. 4).In 2004, a new simple method measuring the GTPase activity is developed (ref. 1), and proved that Bradeion is GTPase, a center of the energy supply system to enable accelerated cell division of a cancer cell. We also succeeded the crystallization of Bradeion for structure analysis of the protein (ref. 2) by the technology described (ref 3). Now we are performing structure analysis of the crystal, and searching for an active site-specific inhibitor. Furthermore, we made monoclonal antibodies against the GTPase of Bradeion identified by our group. We performed immunohistochemistry using the monoclonal antibodies against the whole region or GTPase of Bradeion protein in cultured colorectal carcinoma cells and patients' tissue and studied cancer-specificity and location. We designed the RNAi specific to the nucleotide sequence of Bradeion, using RNA interference technology for as one of the therapy technology, and introduced it into cultured colorectal carcinoma cells and examined influence to them. Now we are investigating influence to them with an adenovirus vector.

这项研究的目的是通过我们的研究小组新发现的癌细胞特异性表达材料开发结直肠癌的诊断技术，并最终开发了新的治疗技术，包括癌细胞靶向医学，我们证明了Bradeion Molecule（A基因和蛋白质）的实用性，用于通过两种诊断技术，可通过两种诊断技术来诊断出corlectal carcinama carcins carcins carcins carcinsy carcins carcins carcinsy;实时RT-PCR测量表达的胸腺基因（在专利期间）和免疫色谱学（测试条方法）检测具有单克隆抗体的标本bradeion蛋白（参考文献4）。癌细胞的分裂。我们还成功地通过描述的技术（参考文献3）成功地结晶了蛋白质的结构分析（参考文献2）。现在，我们正在进行晶体的结构分析，并寻找活性位点特异性抑制剂。此外，我们对我们小组确定的Bradeion GTPase进行了单克隆抗体。我们使用针对整个区域的单克隆抗体或在培养的结直肠癌细胞和患者组织中对整个区域或GTPase进行了免疫组织化学，并研究了癌症特异性和位置。我们将Bradeion核苷酸序列的RNAi设计为一种治疗技术之一，并将其引入了培养的结直肠癌细胞中，并检查了对它们的影响。现在，我们正在用腺病毒载体对他们进行影响。

项目成果

期刊论文数量（32）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Targeting and monitoring of cancer by Bradeion project - Is it possible to control a disease by single molecule monitoring?

Bradeion 项目对癌症进行靶向和监测 - 是否可以通过单分子监测来控制疾病？

DOI：
发表时间：
2003
期刊：
Nature 426(6966)
影响因子：
0
作者：
Tanaka M;Tanaka T;Tamiya E;Yui T;Matsuyama Y.
通讯作者：
Matsuyama Y.

Capillary electrophoresis-based method for in vitro monitoring of GTPase/ATPase activity and enzyme kinetics studies

基于毛细管电泳的 GTPase/ATPase 活性体外监测和酶动力学研究方法

DOI：
发表时间：
2005
期刊：
Analytical and Bioanalytical Chemistry (in press)
影响因子：
0
作者：
Hillebrand S;Carcia W;Cantu MD;Ulian de Araujo;Tanaka M;Tanaka;T. Garrat RC;Carrilho E.
通讯作者：
Carrilho E.

Characterization of the GTPase activity in the recombinant Human Septin SEP4 (Bradeion β)

重组人 Septin SEP4 (Bladeion β) 中 GTP 酶活性的表征