Kinetics of neutrophils and the involvement of cytokines in acute cadmium intoxication.

大鼠皮下注射镉3.0mg/kg，小鼠皮下注射镉2.5mg/kg，外周血中中性粒细胞数量增加，并在注射后18小时达到峰值水平，小鼠嗜中性粒细胞数量比大鼠强。沿着嗜中性粒细胞增多，G-SCF水平也随之增高，18 h达高峰，与中性粒细胞数量的时间进程相一致。此外，两个阶段的增加GM SCF在外周血中，虽然水平是非常小的，观察到在小鼠镉。相反，嗜中性粒细胞早于镉，注射后12小时的峰值，诱导在小鼠腹腔注射LPS在0.5，或5 mg/kg。与此相一致，LPS注射后外周血G-CSF水平升高的时间早于Cd。换句话说，G-CSF升高的时间过程存在显著差异。注射LPS后，小鼠外周血中GM-CSF也出现与Cd不同的升高模式，即注射LPS后2 h GM-CSF急剧升高，用中和LPS的抗生素多粘菌素B预处理可有效抑制LPS诱导的G-CSF升高，但对Cd诱导的G-CSF升高无明显影响。如嗜中性粒细胞和G-CSF或GM-CSF的诱导，通过与LPS诱导的一般炎症不同的机制来管理。

When cadmium (Cd) was injected into rats at 3.0 mg/kg, or into mice at 2.5 m,g.kg s.c., the numbers of neutrophils in peripheral blood were increased and reached the peak levels 18 hours after injection, and the magnitude of the neutrophilia was stronger in mice than rats. Along with the neutrophilia, the level of G-SCF was increased so much and reached the peak at 18 hours, corresponding with the time course of the number of neutrophils. In addition, the two-phase increase of GM SCF in peripheral blood, although the level was very small, was observed in mice administered Cd.On the contrary, neutrophilia with earlier peaks than Cd, 12 hours after injection, was induced in mice that were administered LPS at 0.5, or 5 mg/kg intraperitoneously. In accordance with that, G-CSF in peripheral blood was increased at much earlier time after LPS injection thatn Cd. In other words, there was significant difference in the time course of G-CSF increase. GM-CSF in peripheral blood also showed a different pattern of increase from Cd after injection of LPS, which was a sharp increase of GM-CSF 2 hours after injection.The pretreatment of polymyxin B, an antibiotics to neutralize LPS, inhibited the LPS-induced G-CSF increase effectively, but did not the Cd-induced one in mice.These results suggest that Cd-induced acute inflammation, such as neutrophilia and the induction of G-CSF or GM-CSF, was managed through mechanism different from the LPS-induced general inflammation.