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S100 protein as a marker of central nerves injury

S100蛋白作为中枢神经损伤的标志物

基本信息

批准号：
15590585
负责人：
ZHU Bao-Li
金额：
$ 2.05万
依托单位：
Osaka City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

To evaluate the severity of cerebral damage, this study investigated the immunohistochemical distribution of S100 protein in cerebral cortex in forensic autopsy cases (n=300) with regard to the cause of death and relationship to serum S100B protein levels.The serum S100B level was usually higher in the subclavian vein than in the right heart and external iliac vein, and lowest in the left, heart blood, showing no significant postmortem influence within 48 h postmortem. The serum level was markedly higher for acute deaths from head injury and asphyxiation due to neck compression (strangulation and hanging). For head injury cases, the serum level was dependent on the severity of the brain injury and survival ; it was significantly higher for the cases of peracute and acute deaths due to head injury than for longer survival cases.S100 immunopositivity was mainly observed in the gliacytes, in part, in the neurons and myelin. S100 positivity in the astrocytes was dependent on the cause of death, and was significantly lower in acute deaths due to strangulation/hanging and drowning, and mildly low in those due to injuries. S100 positivity in neurons and myelins was frequently observed in delayed head injury deaths and fire fatalities, showing an inverse relationship with the positivity in astrocytes in head injury cases. For cases of acute death, there was an inverse relationship between S100 positivity in the astrocytes and the serum S100B level.The above observations suggest that combined analyses of S100 positivity in the brain and serum S100B levels may be useful for investigation death due to head injury, hypoxic and/or ischemic cerebral damage.

为了评估脑损伤的严重程度，本研究调查了法医尸检病例（n=300）大脑皮层中S100蛋白的免疫组织化学分布与死亡原因及其与血清S100B蛋白水平的关系。锁骨下静脉血清S100B水平通常高于右心和髂外静脉，而左心血液最低，在死后48 h内无明显的死后影响。由于头部损伤和颈部压迫（勒死和上吊）导致的窒息造成的急性死亡，血清水平明显较高。对于颅脑损伤病例，血清水平与颅脑损伤严重程度和生存率有关；头部损伤引起的过急性和急性死亡病例的死亡率明显高于存活时间较长的病例。S100免疫阳性主要见于胶质细胞，部分见于神经元和髓磷脂。星形胶质细胞中的S100阳性取决于死亡原因，在因勒死/上吊和溺水而导致的急性死亡中，S100阳性明显较低，在因受伤而导致的急性死亡中，S100阳性略低。在迟发性头部损伤死亡和火灾死亡中，经常观察到神经元和髓鞘中S100阳性，这与头部损伤病例中星形胶质细胞的阳性呈反比关系。在急性死亡病例中，星形胶质细胞中S100阳性与血清S100B水平呈负相关。上述观察结果表明，脑S100阳性和血清S100B水平的联合分析可能有助于调查脑损伤、缺氧和/或缺血性脑损伤导致的死亡。