New Liquid-Liquid Extraction Method using Hydrophilic Organic Solvent

通过对几种溶剂和盐类的考察，以及pH对建立亲水性有机溶剂液-液萃取新方法的影响，确定了几种亲水性有机溶剂和盐类对该方法的适用性。对于液质联用，醋酸铵对样品中药物的盐析效果最好。这些发现随后被应用于苯二氮类药物、巴比妥酸盐和降压药物的鉴定和定量，如巴比妥、苯巴比妥、戊巴比妥、赛可巴比妥、硝西潘、地西潘、溴代戊脲、阿普诺利和哌唑嗪。此外，这种新的液液提取方法被应用于胃内容物和血清标本的分选试验。用该方法提取的样品的检测结果与高效液相色谱定量结果的相关性要好于未提取的样品。

Following investigation of several solvents and salts and the effect of pH to establish the new liquid-liquid extraction method using hydrophilic organic solvent, several pairs of the hydrophilic organic solvents and salts appeared suitable for the method. For liquid chromatography-mass spectrometry, ammonium acetate appeared most effective to salt out the drugs in the samples. These findings were then applied to identification and quantitation of benzodiazepines, barbiturates and antihypertensive medicines, such as barbital, phenobarbital, pentobarbital, secobarbital, nitrazepam, diazepam, bromovalerylurea, apronalide and prazosin. Furthermore, this new liquid-liquid extraction method was applied to the Triage test using gastric contents and serum specimens. The test results using samples extracted by the method was correlated better with the quantitative results by high performance liquid chromatography than those using unextracted samples.