Autologous Bone-marrow Mononuclear Cell Implantation in Patients with Peripheral Artery Diseases : Mesenchymal Stem Cell Acute Culture Method and Assessment of Endothelial Function in Angiogenesis

外周动脉疾病患者的自体骨髓单核细胞移植：间充质干细胞急性培养方法及血管生成中内皮功能的评估

• 批准号: 15590751
• 负责人: HIGASHI Yukihito
• 金额: $ 2.05万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590751/
• 关键词: Autologous bone-marrow mononuclear cell implantation; Endothelial function; Angiogenesis; Mesenchymal stem cell; Arteriosclerotic obliterance; Burger disease; Endothelial progenitor cell; Auto cell culture machine; 細胞自動培養増殖装置

1.Limb ischemia is associated with endothelial dysfunction. To determine whether autologous bone-marrow mononuclear cell (BM-MNC) implantation improves endothelial function in patients with limb ischemia, we evaluated leg blood flow (LBF) responses to acetylcholine (ACh), an endothelium-dependent vasodilator, and sodium nitroprusside (SNP), an endothelium-independent vasodilator, using a mercury-filled silastic strain-gauge plethysmography before and after BM-MNC implantation in 7 patients with limb ischemia. After BM-MNC implantation, LBF response to ACh was enhanced. The vasodilatory effects of SNP before and after BM-MNC implantation were similar. These findings suggest that BM-MNC implantation augments endothelium-dependent vasodilation in patients with limb ischemia. (Circulation. 2004)2.Although it is clear that BM-MNC implantation is useful for patients with severe limb ischemia, patients were subjected by general anesthesia during BM-MNC aspiration. In the present study, we established the autologous mesenchymal stem cell culture method. We are making a plan to perform the autologous mesenchymal stem cell implantation in patients with limb ischemia.

1.肢体缺血与内皮功能障碍有关。为了确定自体骨髓单个核细胞（BM-MNC）植入是否改善肢体缺血患者的内皮功能，我们在7例肢体缺血患者BM-MNC植入前后使用汞填充硅橡胶应变计体积描记法评估了腿部血流（LBF）对乙酰胆碱（ACh）（一种内皮依赖性血管扩张剂）和硝普钠（SNP）（一种内皮非依赖性血管扩张剂）的反应。BM-MNC植入后，LBF对ACh的反应增强。SNP在BM-MNC植入前后的血管舒张作用相似。这些结果表明，骨髓单个核细胞植入增强血管内皮依赖性舒张肢体缺血患者。（循环。2004)2.尽管BM-MNC植入对于严重肢体缺血患者是有用的，但在BM-MNC抽吸期间患者接受全身麻醉。本研究建立了自体骨髓间充质干细胞的培养方法。我们正在制定一个计划，在肢体缺血患者中进行自体间充质干细胞植入。

项目成果

Molecular markers distinguish bone marrow mesenchymal stem cells from fibroblasts

• DOI: 10.1016/j.bbrc.2005.04.118
• 发表时间: 2005-06-24
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Ishii, M;Koike, C;Kato, Y
• 通讯作者: Kato, Y

Azelnidipine : a viewpoint by Yukihito Higashi (guest commentaries).

阿泽尼地平：东幸仁的观点（嘉宾评论）。

• 发表时间: 2003
• 期刊: Drugs 63
• 作者: Tsuda M;Higashi Y(他7名;3番目);Higashi Y;Sanada M;Sanada M;Noma K;Kimura M;Goto C;Teragawa H;Matsuda K;Oshima T;Higashi Y
• 通讯作者: Higashi Y

Response of the left anterior descending coronary artery to acetylcholine in patients with chest pain and angiographically normal coronary arteries.

胸痛和冠状动脉造影正常的患者左冠状动脉前降支对乙酰胆碱的反应。

• 发表时间: 2003
• 期刊: Am J Cardiol 92
• 作者: Tsuda M;Higashi Y(他7名;3番目);Higashi Y;Sanada M;Sanada M;Noma K;Kimura M;Goto C;Teragawa H;Matsuda K;Oshima T;Higashi Y;Matsuda K
• 通讯作者: Matsuda K

Method for assessing endothelial function in humans using a strain-gauge plethysmography : nitric oxide-dependent and -independent vasodilation.

使用应变计体积描记法评估人体内皮功能的方法：一氧化氮依赖性和非依赖性血管舒张。

• 发表时间: 2003
• 期刊: J Pharma Sci 93
• 作者: Tsuda M;Higashi Y(他7名;3番目);Higashi Y;Sanada M;Sanada M;Noma K;Kimura M;Goto C;Teragawa H;Matsuda K;Oshima T;Higashi Y;Matsuda K;Higashi Y;Goto C;Oshima T;Higashi Y
• 通讯作者: Higashi Y

Exercise and endothelial function : role of endothelium-derived nitric oxide and oxidative stress in healthy subjects and hypertensive patients (review).

运动和内皮功能：内皮源性一氧化氮和氧化应激在健康受试者和高血压患者中的作用（综述）。

• 发表时间: 2004
• 期刊: Pharmacology and Therapeutics 102
• 作者: Oshima T;Higashi Y(他8名;6番目);Higashi Y(他10名);Higashi Y;Higashi Y
• 通讯作者: Higashi Y