Autologous Bone-marrow Mononuclear Cell Implantation in Patients with Peripheral Artery Diseases : Mesenchymal Stem Cell Acute Culture Method and Assessment of Endothelial Function in Angiogenesis

1.肢体缺血与内皮功能障碍相关。为了确定自体骨髓单个核细胞(BM-MNC)植入是否能改善肢体缺血患者的内皮功能，我们用充满汞的硅胶应变片体积描记仪，评估了7例肢体缺血患者植入BM-MNC前后小腿血流(LBF)对血管内皮依赖性扩张剂乙酰胆碱(ACh)和硝普钠(SNP)的反应。植入BM-MNC后，LBF对ACh的反应增强。SNP在BM-MNC植入前后的血管扩张作用相似。这些发现表明，植入BM-MNC可增强肢体缺血患者的内皮依赖性血管扩张。(发行量。尽管BM-MNC植入术对严重肢体缺血的患者是有效的，但患者在BM-MNC抽吸术中接受全身麻醉。本研究建立了自体骨髓间充质干细胞的培养方法。我们正在制定计划，为肢体缺血患者进行自体骨髓间充质干细胞移植。

1.Limb ischemia is associated with endothelial dysfunction. To determine whether autologous bone-marrow mononuclear cell (BM-MNC) implantation improves endothelial function in patients with limb ischemia, we evaluated leg blood flow (LBF) responses to acetylcholine (ACh), an endothelium-dependent vasodilator, and sodium nitroprusside (SNP), an endothelium-independent vasodilator, using a mercury-filled silastic strain-gauge plethysmography before and after BM-MNC implantation in 7 patients with limb ischemia. After BM-MNC implantation, LBF response to ACh was enhanced. The vasodilatory effects of SNP before and after BM-MNC implantation were similar. These findings suggest that BM-MNC implantation augments endothelium-dependent vasodilation in patients with limb ischemia. (Circulation. 2004)2.Although it is clear that BM-MNC implantation is useful for patients with severe limb ischemia, patients were subjected by general anesthesia during BM-MNC aspiration. In the present study, we established the autologous mesenchymal stem cell culture method. We are making a plan to perform the autologous mesenchymal stem cell implantation in patients with limb ischemia.