Autologous Bone-marrow Mononuclear Cell Implantation in Patients with Peripheral Artery Diseases : Mesenchymal Stem Cell Acute Culture Method and Assessment of Endothelial Function in Angiogenesis

1.肢体缺血与内皮功能障碍有关。为了确定自体骨髓单个核细胞（BM-MNC）植入是否改善肢体缺血患者的内皮功能，我们在7例肢体缺血患者BM-MNC植入前后使用汞填充硅橡胶应变计体积描记法评估了腿部血流（LBF）对乙酰胆碱（ACh）（一种内皮依赖性血管扩张剂）和硝普钠（SNP）（一种内皮非依赖性血管扩张剂）的反应。BM-MNC植入后，LBF对ACh的反应增强。SNP在BM-MNC植入前后的血管舒张作用相似。这些结果表明，骨髓单个核细胞植入增强血管内皮依赖性舒张肢体缺血患者。（循环。2004)2.尽管BM-MNC植入对于严重肢体缺血患者是有用的，但在BM-MNC抽吸期间患者接受全身麻醉。本研究建立了自体骨髓间充质干细胞的培养方法。我们正在制定一个计划，在肢体缺血患者中进行自体间充质干细胞植入。

1.Limb ischemia is associated with endothelial dysfunction. To determine whether autologous bone-marrow mononuclear cell (BM-MNC) implantation improves endothelial function in patients with limb ischemia, we evaluated leg blood flow (LBF) responses to acetylcholine (ACh), an endothelium-dependent vasodilator, and sodium nitroprusside (SNP), an endothelium-independent vasodilator, using a mercury-filled silastic strain-gauge plethysmography before and after BM-MNC implantation in 7 patients with limb ischemia. After BM-MNC implantation, LBF response to ACh was enhanced. The vasodilatory effects of SNP before and after BM-MNC implantation were similar. These findings suggest that BM-MNC implantation augments endothelium-dependent vasodilation in patients with limb ischemia. (Circulation. 2004)2.Although it is clear that BM-MNC implantation is useful for patients with severe limb ischemia, patients were subjected by general anesthesia during BM-MNC aspiration. In the present study, we established the autologous mesenchymal stem cell culture method. We are making a plan to perform the autologous mesenchymal stem cell implantation in patients with limb ischemia.