REGULATION OF HYPERTENSION-INDUCED RENAL INJURY BY INDUCTION OF SMOOTH MUSCLE ACTIN BINDING PROTEIN EXPRESSION

自发性高血压大鼠（SHR）的平滑肌肌动蛋白结合蛋白碱性钙蛋白（bCN-Tg）的过表达已被证实。与野生型SHR相比，bCN-Tg组高血压发病延迟。分别培养bCN-Tg和野生型SHR系膜细胞。与野生型SHR相比，bCN-Tg系膜细胞中PDGF-BB诱导的增殖活性明显受到抑制。由此可见，bCN诱导抑制了高血压的发生和肾系膜细胞的增殖，这可能对高血压和高血压相关肾损伤的治疗有益。为了研究bCN在高血压肾损伤中的作用，我们在bCN和野生型SHR中诱导抗Thy-1肾炎。与野生黑麦SHR相比，bCN抗hy-1肾炎小鼠肾小球细胞增殖和系膜细胞活化均受到显著抑制。bCN也显著抑制了肾小球中IV型胶原的积累。总之，bCN诱导将是治疗高血压和高血压相关肾损伤的有效策略。

Trasnsgenic for overexpression of basic calponin (bCN-Tg), smooth muscle actin binding protein, has been established in spontaneous hypertension rat (SHR). Onset of hypertension delayed in bCN-Tg compared to wild type SHR. Mesangial cells from both bCN-Tg and wild type SHR were cultured. Proliferative activity induced by PDGF-BB was significantly suppressed in mesangial cells from bCN-Tg than those from wild type SHR. From these, bCN induction resulted in suppression of the onset of hypertension and mesangial cell proliferation, that may be beneficial for the treatment of both hypertension and hypertension-related renal injuries. To study the role of bCN in renal injury associated with hypertension, anti Thy-1 nephritis was induced in both bCN and wild type SHR. Both glomerular cell proliferation and activation of mesangial cells evaluated by the expression of α-smooth muscle actin were significantly suppressed in anti Thy-1 nephritis in bCN compared to wild rye SHR. Subsequent type IV collagen accumulation in glomeruli was also significantly suppressed in bCN. In conclusion, bCN induction will be a powerful strategy for hypertension and hypertension associated renal injuries.