Detailed mechanisms of signal transduction pathways of AngII in chromaffin cells

血管紧张素II受体AT_1和AT_2是两种截然不同的受体。我们先前已经证明，刺激AT_2会降低培养的猪嗜铬细胞内cGMP水平，其中AT_2是主要表达的受体。然而，AT_1或AT_2是否影响嗜铬细胞的信号转导通路尚不清楚。利用基因芯片技术，比较了AngiI+CV-11974(模拟AT_2刺激的AT1拮抗剂)和AngiI+PD 123319(模拟AT_1刺激的AT_2拮抗剂)的作用。在这种情况下，有几个基因表达下调和过度表达。我们正在描述这些基因的特征。

Two distinct types of angiotensin II (AngII) receptors, AT_1 and AT_2, have been cloned. We have previously shown that stimulation of AT_2 reduces intracellular cGMP levels in cultured porcine chromaffin cells in which AT_2 is the predominantly expressed receptor. However, it has not been determined whether AT_1 or AT_2 affects signal transduction pathways in chromaffin cells. Using Genetic Micro System(GMS-418-array scanner), we compare the effects of AngII plus CV-11974 (an AT_1 antagonist, which simulates specific AT_2 stimulation) with AngII plus PD 123319 (an AT_2 antagonist, which simulates specific AT_1 stimulation). There are several genes down and over expressed under such condition. We are characterize these genes.