Role of chemokine CCL20 and its receptor CCR6 against bacterial infection.

趋化因子 CCL20 及其受体 CCR6 对抗细菌感染的作用。

• 批准号: 15591070
• 负责人: MATSUMURA Haruo
• 金额: $ 2.24万
• 依托单位: Kinki University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591070/
• 关键词: infection P; O; oral immunization; hamster; Salmonella; monoclonal antibody; mucosal immunity; eye; cornea; ハイブリドーマ

This research is to investigate the role of the chemokine CCL20-CCR6 system at intestine and lymphoid organ in mice infected with Salmonella and other microorganisms We used non-immune mice and mice immunized perorally with avirulent strain of Salmonella typhimurium for the analysis of primary immune response and secondary immune response, respectively, after challenge with the virulent strain of Salmonella typhimurium.Using immunized Armenian hamster spleen cells, we established anti-mouse CCR6 monoclonal antibody termed 29.2L17. The monoclonal antibody 29.2L17 was specific for CCR6 among the 18 mouse chemokine receptors and capable of staining normal mouse lymphoid cells known to expree CCR6. Furthermore, 29.2L17 could be used for staining cells expressing CCR6 in mouse tissues by immunohistochemistry. However, 29.2L17 does not have a significant neutralizing activity against mouse CCR6.We established experimental conditions for peroral immunization and peroral challenge of Salmonella typhimurium. Using these conditions, intestinal tract, liver and spleen were collected from control or immunized mice before or after the challenge with Salmonella typhimurium at several time points. These tissues are useful for the analysis of the role of the CCL20-CCR6 system in primary and secondary immune responses in the mucosal immunity.We have also demonstrated the ability of corneal epithelial cells and stromal keratocytes to produce CCL20 in vitro and in vivo. By using a mouse model of herpetic stromal keratatis, it has been revealed that not only epithelial cells but also stromal keratocytes efficiently produce CCL20 in the cornea and recruit cells expressing CCR6 such as dendritic sells into inflamed cornea.

本研究旨在探讨趋化因子CCL 20-CCR 6系统在沙门氏菌等微生物感染小鼠肠道和淋巴器官中的作用。采用非免疫小鼠和经口免疫鼠伤寒沙门氏菌弱毒株的小鼠，分别进行初次免疫应答和二次免疫应答分析，用免疫的亚美尼亚仓鼠脾细胞制备了抗小鼠CCR 6的单克隆抗体29.2L17。单克隆抗体29.2L17对18种小鼠趋化因子受体中的CCR 6具有特异性，并且能够染色已知表达CCR 6的正常小鼠淋巴细胞。此外，29.2L17可用于免疫组织化学染色小鼠组织中表达CCR 6的细胞。但29.2L17对小鼠CCR 6无明显的中和活性。使用这些条件，在几个时间点用鼠伤寒沙门氏菌攻击之前或之后，从对照或免疫小鼠收集肠道、肝脏和脾脏。这些组织可用于分析CCL 20-CCR 6系统在粘膜免疫中的原发性和继发性免疫应答中的作用，我们还在体外和体内证实了角膜上皮细胞和基质角膜细胞产生CCL 20的能力。通过使用疱疹性基质角膜炎的小鼠模型，已经揭示了不仅上皮细胞而且基质角膜细胞在角膜中有效地产生CCL 20，并将表达CCR 6的细胞如树突状细胞募集到发炎的角膜中。

项目成果

Corneal epithelial cells and stromal keratocytes efficientl produce CC chemokine-ligand 20(CCL20) and attract cell expressing its receptor CCR6 in mouse herpetic stroma keratitis.

在小鼠疱疹性基质角膜炎中，角膜上皮细胞和基质角膜细胞有效产生CC趋化因子配体20（CCL20）并吸引表达其受体CCR6的细胞。

• 发表时间: 2004
• 期刊: Current Eye Research 28
• 作者: J.Shirane et al.

Cutting Edge : Profile of chemokine receptor expression o human plasma cells accounts for their efficient recruitmen to target tissues.

前沿：人类浆细胞趋化因子受体表达概况解释了它们有效招募目标组织的原因。

• 发表时间: 2003
• 期刊: The Journal of Immunology 170
• 作者: Jumi Shirane;Takashi Nakayama;Daisuke Nagakubo;Dai Izawa;Kunio Hieshima;Yosikazu Shimomura;Osamu Yoshie;J.Shirane et al.;T.Nakayama et al.
• 通讯作者: T.Nakayama et al.

Cutting edge: Profile of chemokine receptor expression on human plasma cells accounts for their efficient recruitment to target tissues

• DOI: 10.4049/jimmunol.170.3.1136
• 发表时间: 2003-02-01
• 期刊: JOURNAL OF IMMUNOLOGY
• 影响因子: 4.4
• 作者: Nakayama, T;Hieshima, K;Yoshie, O
• 通讯作者: Yoshie, O

Corneal epithelial cells and stromal keratocytes efficiently produce CC chemokine-ligand 20 (CCL20) and attract cells expressing its receptor CCR6 in mouse herpetic stromal keratitis.

在小鼠疱疹性基质角膜炎中，角膜上皮细胞和基质角膜细胞有效产生 CC 趋化因子配体 20 (CCL20) 并吸引表达其受体 CCR6 的细胞。

• 发表时间: 2004
• 期刊: Current Eye Research vol.28
• 作者: Jumi Shirane;Takashi Nakayama;Daisuke Nagakubo;Dai Izawa;Kunio Hieshima;Yosikazu Shimomura;Osamu Yoshie
• 通讯作者: Osamu Yoshie

Corneal epithelial cells and stromal keratocytes efficiently produce CC chemokine-ligand 20 (CCL20) and attract cell expressing its receptor CCR6 in mouse herpetic stromal keratitis

小鼠疱疹性基质角膜炎中角膜上皮细胞和基质角膜细胞有效产生 CC 趋化因子配体 20 (CCL20) 并吸引表达其受体 CCR6 的细胞

• 发表时间: 2004
• 期刊: Current Eye Research 28
• 作者: Jumi Shirane;Takashi Nakayama;Daisuke Nagakubo;Dai Izawa;Kunio Hieshima;Yosikazu Shimomura;Osamu Yoshie;J.Shirane et al.
• 通讯作者: J.Shirane et al.